表达识别脂壳寡糖的异源受体的基因改变的植物【掌桥专利】 |
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相关申请的交叉引用 本申请要求于2018年8月13日提交的美国临时申请号62/718,186的权益,其通过引用以其整体并入本文。 作为ASCII文本文件提交序列表 以下ASCII文本文件的提交内容通过引用以其整体并入本文:序列表的计算机可读形式(CRF)(文件名:794542000340SEQLIST.txt,记录日期:2019年8月12日,大小:253KB)。 技术领域 本公开涉及基因改变的植物。具体地,本公开涉及含有编码异源受体多肽的核酸序列的基因改变的植物。植物能够通过异源受体多肽识别脂壳寡糖(LCO)。 背景技术 植物在其环境中暴露于良性和病原性的各种微生物。为了防御病原性微生物,植物具有通过大量受体识别微生物的特定分子信号的能力,并且取决于信号的模式,可启动适当的免疫应答。分子信号源自微生物的分泌材料、细胞壁组分和甚至胞质蛋白。壳寡糖(CO)是植物通过质膜上发现的几丁质受体CEBiP和CERK1识别的重要的真菌分子信号。这些受体在LysM类别的受体中,并且识别来自真菌的CO的尺寸和乙酰化。脂壳寡糖(LCO)是由细菌和真菌产生的另一重要的分子信号,其被其他LysM受体识别。 除了良性和病原性微生物外,一些微生物可通过缔合或共生对植物有益。与某些固氮细菌和真菌进入共生关系的植物需要能够识别特定的细菌或真菌物种以启动共生,同时仍能够激活其免疫系统以对其他细菌和真菌做出答应。允许植物来识别这些特定的细菌或真菌的一种重要机制是通过特化的LysM受体,其对特定的细菌或真菌产生的LCO形式具有高亲和性和高选择性,而来自其他细菌和真菌的LCO不被这些特化的LysM受体识别。 使用突变体植物和表型输出的功能研究已经用于鉴定这些特化的LysM受体。然而,目前,仅已经在实验上鉴定了来自有限数量的植物物种并且能够识别有限数量的潜在共生体的少数高亲和性和高选择性的LysM受体。由于这些受体是识别共生细菌和真菌物种以及启动共生所必需的,因此重要的是有更多可得到的受体可以用于工程化对另外的共生细菌和真菌物种的识别。需要更广泛的受体用于工程化目前不能形成共生关系的植物中的共生以及用于优化能够形成共生关系的植物中的共生。 发明内容 为了工程化植物-微生物共生关系,明显需要另外的特化的LysM受体。因此,本公开提供了允许植物识别由细菌或真菌物种产生的脂壳寡糖(LCO)的多种新的高亲和性和高选择性LysM受体。 本公开的某些方面涉及含有编码异源受体多肽的核酸序列的基因改变的植物或植物部分,其中异源受体多肽选自下述的组中:与SEQ ID NO:5[鹰嘴豆/鹰嘴豆(Cicerarietinum)NFR5]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第一多肽,与SEQ IDNO:7[菜豆/菜豆(Phaseolus vulgaris)NFR5]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第二多肽,与SEQ ID NO:9[花生/花生属(Arachis)NFR5]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第三多肽,与SEQ ID NO:11[莲属(Lotus)LYS11]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第四多肽,与SEQ ID NO:12[苜蓿属(Medicago)LYR1]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第五多肽,与SEQ ID NO:13[拟山黄麻属(Parasponia)NFP1]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第六多肽,与SEQID NO:16[大麦HvLysM-RLK1(AK370300)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第七多肽,与SEQ ID NO:17[大麦HvLysM-RLK2(AK357612)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第八多肽,与SEQ ID NO:18[大麦HvLysM-RLK3 AK372128]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第九多肽,与SEQ ID NO:19[大麦HvLysM-RLK10(HORVU4Hr1G066170)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十多肽,与SEQID NO:20[玉蜀黍ZM1(XP_020399958)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十一多肽,与SEQ ID NO:21[玉蜀黍ZM5(XP_008652982.1)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十二多肽,与SEQ ID NO:22[苹果NFP5 XP_008338966.1]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十三多肽或与SEQ ID NO:23[草莓NFR5 XP_004300586.2]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十四多肽。在一些实施方式中,异源受体多肽选自下述的组中:SEQ ID NO:5[鹰嘴豆/鹰嘴豆(Cicer arietinum)NFR5]、SEQ ID NO:7[菜豆/菜豆(Phaseolus vulgaris)NFR5]、SEQ ID NO:9[花生/花生属(Arachis)NFR5]、SEQ ID NO:11[莲属(Lotus)LYS11]、SEQ ID NO:12[苜蓿属(Medicago)LYR1]、SEQ ID NO:13[拟山黄麻属(Parasponia)NFP1]、SEQ ID NO:16[大麦HvLysM-RLK1(AK370300)]、SEQ ID NO:17[大麦HvLysM-RLK2(AK357612)]、SEQ ID NO:18[大麦HvLysM-RLK3AK372128]、SEQ ID NO:19[大麦HvLysM-RLK10(HORVU4Hr1G066170)]、SEQ ID NO:20[玉蜀黍ZM1(XP_020399958)]、SEQ ID NO:21[玉蜀黍ZM5(XP_008652982.1)]、SEQ ID NO:22[苹果NFP5 XP_008338966.1]和SEQ ID NO:23[草莓NFR5 XP_004300586.2]。在一些实施方式中,异源受体多肽的表达允许植物或其部分通过异源受体多肽识别脂壳寡糖(LCO)。在一些实施方式中,LCO由固氮菌或由菌根真菌产生。在一些实施方式中,LCO由选自下述的组中的固氮菌产生:百脉根中慢生根瘤菌(Mesorhizobium loti)、华癸中慢生根瘤菌(Mesorhizobium huakuii)、地中海中慢生根瘤菌(Mesorhizobium mediterraneum)、鹰嘴豆中慢生根瘤菌(Mesorhizobium ciceri)、中慢生根瘤菌属(Mesorhizobium)物种、蒙古根瘤菌(Rhizobium mongolense)、热带根瘤菌(Rhizobium tropici)、菜豆埃特里根瘤菌(Rhizobium etli phaseoli)、贾氏根瘤菌(Rhizobium giardinii)、豌豆根瘤菌(Rhizobium leguminosarum)任选地三叶草豌豆根瘤菌(R.leguminosarum trifolii)、蚕豆豌豆根瘤菌(R.leguminosarum viciae)和菜豆豌豆根瘤菌(R.leguminosarumphaseoli)、伯克氏菌目(Burkholderiales)任选地含羞草的共生体、苜蓿中华根瘤菌(Sinorhizobium meliloti)、药用中华根瘤菌(Sinorhizobium medicae)、费氏中华根瘤菌(Sinorhizobium fredii)、费氏中华根瘤菌(Sinorhizobium fredii)NGR234、茎瘤固氮根瘤菌(Azorhizobium caulinodans)、大豆慢生根瘤菌(Bradyrhizobium japonicum)、埃氏慢生根瘤菌(Bradyrhizobium elkanii)、辽参慢生根瘤菌(Bradyrhizobiumliaonginense)、根瘤菌属(Rhizobium)物种、中慢生根瘤菌属(Mesorhizobium)物种、中华根瘤菌属(Sinorhizobium)物种、固氮根瘤菌属(Azorhizobium)物种、弗兰克氏菌属(Frankia)物种或其任何组合,或由选自下述的组中的菌根真菌产生:无柄孢子科(Acaulosporaceae)物种、多孢囊霉科(Diversisporaceae)物种、巨孢囊霉科(Gigasporaceae)物种、平囊霉科(Pacisporaceae)物种、管柄囊霉属(Funneliformis)物种、球囊霉属(Glomus)物种、球囊霉属(Rhizophagus)物种、硬囊霉属(Sclerocystis)物种、Septoglomus属物种、Claroideoglomus属物种、Ambispora属物种、原囊霉属(Archaeospora)物种、Geosiphon pyriformis属物种、类球囊霉属(Paraglomus)属物种、球囊霉门(Glomeromycota)中的其他物种或其任何组合。在一些实施方式中,异源多肽位于植物细胞质膜。在一些实施方式中,植物细胞是根细胞。在一些实施方式中,根细胞是根表皮细胞或根皮质细胞。在一些实施方式中,异源多肽在发育中的植物根系中表达。在一些实施方式中,核酸序列可操作地连接至启动子。在一些实施方式中,启动子是根特异性启动子。在一些实施方式中,启动子选自下述的组中:NFR1或NFR5/NFP启动子、莲属(Lotus)NFR5启动子(SEQ ID NO:24)和莲属(Lotus)NFR1启动子(SEQ ID NO:25)、玉蜀黍甲硫氨酸启动子、几丁质酶启动子、玉蜀黍ZRP2启动子、番茄LeExtl启动子、谷氨酰胺合酶大豆根启动子、RCC3启动子、水稻antiquitine启动子、LRR受体激酶启动子或拟南芥pCO2启动子。在一些实施方式中,启动子是任选地选自下述的组中的组成型启动子:CaMV35S启动子、CaMV35S启动子的衍生物、玉蜀黍泛素启动子、三叶启动子、静脉花叶木薯病毒启动子或拟南芥UBQ10启动子。在一些实施方式中,植物选自下述的组中:玉米(例如,玉蜀黍、玉米(Zea mays)),水稻(例如,亚洲栽培稻(Oryza sativa)、非洲栽培稻(Oryza glaberrima)、菰属(Zizania)物种,大麦(例如,大麦(Hordeum vulgare),小麦(例如,普通小麦、斯卑尔脱小麦、硬粒小麦、普通小麦(Triticum aestivum)、斯卑尔脱小麦(Triticum spelta)、硬粒小麦(Triticumdurum)、小麦属(Triticum)物种),山黄麻属(Trema)物种(例如,光叶山黄麻(Tremacannabina)、Trema cubense、Trema discolor、Trema domingensis、Trema integerrima、Trema lamarckiana、小花山黄麻(Trema micrantha)、异色山黄麻(Trema orientalis)、Trema philippinensis、Trema strigilosa、山黄麻(Trema tomentosa)),苹果(例如,西洋苹果(Malus pumila),梨(例如,西洋梨(Pyrus communis)、白梨(Pyrus×bretschneideri)、沙梨(Pyrus pyrifolia)、新疆梨(Pyrus sinkiangensis)、川梨(Pyruspashia)、梨属(Pyrus)物种),李子(例如,西梅、西洋李子,布拉斯李、欧洲李(Prunusdomestica)、中国李(Prunus salicina)),杏子(例如,山杏(Prunus armeniaca)、布里扬松杏(Prunus brigantina)、东北杏(Prunus mandshurica)、梅(Prunus mume)、西伯利亚杏(Prunus sibirica)),桃子(例如,油桃、桃(Prunus persica),扁桃(例如,甜扁桃(Prunusdulcis)、扁桃(Prunus amygdalus)),胡桃(例如,波斯胡桃、英国胡桃、黑胡桃、胡桃(Juglans regia)、黑胡桃(Juglans nigra)、白胡桃(Juglans cinerea)、加州胡桃(Juglans californica)),草莓(例如,草莓(Fragaria×ananassa)、智利草莓(Fragariachiloensis)、弗州草莓(Fragaria virginiana)、野草莓(Fragaria vesca)),树莓(例如,欧洲红树莓、黑树莓、覆盆子(Rubus idaeus)、美国黑树莓(Rubus occidentalis)、硬毛树莓(Rubus strigosus)),黑莓(例如,常青黑莓,喜马拉雅黑莓、黑莓(Rubus fruticosus)、北美黑莓(Rubus ursinus)、裂叶黑莓(Rubus laciniatus)、尖齿黑莓(Rubus argutus)、亚美尼亚黑莓(Rubus armeniacus)、露莓(Rubus plicatus)、榆叶黑莓(Rubus ulmifolius)、美国黑莓(Rubus allegheniensis)),红醋栗(例如,红丛茶蔍子(Ribes rubrum)、红茶藨子(Ribes spicatum)、高山茶藨子(Ribesbes alpinum)、Ribes schlechtendalii、多花茶藨子(Ribes multiflorum)、岩生茶藨子(Ribes petraeum)、伏生茶藨子(Ribes triste)),黑醋栗(例如,黑茶藨子(Ribes nigrum),甜瓜(例如,西瓜、冬瓜、卡萨巴甜瓜、哈密瓜、白兰瓜、香瓜、西瓜(Citrullus lanatus)、冬瓜(Benincasa hispida)、罗马甜瓜(Cucumis melocantalupensis)、冬甜瓜(Cucumis melo inodorus)、网纹甜瓜(Cucumis meloreticulatus)),黄瓜(例如,切黄瓜、腌制黄瓜、英国黄瓜、黄瓜(Cucumis sativus)),南瓜(例如,美国南瓜(Cucurbita pepo)、笋瓜(Cucurbita maxima)),倭瓜(例如,葫芦、西葫芦(Cucurbita argyrosperma)、黑子南瓜(Cucurbita ficifolia)、笋瓜(Cucurbitamaxima)、中国南瓜(Cucurbita moschata)),葡萄(例如,葡萄(Vitis vinifera)、山葡萄(Vitis amurensis)、美洲葡萄(Vitis labrusca)、白亮葡萄(Vitis mustangensis)、河岸葡萄(Vitis riparia)、圆叶葡萄(Vitis rotundifolia))或大麻(例如,印度大麻、大麻(Cannabis sativa))。 在任何以上实施方式的一些实施方式中:植物部分是叶、茎、根、根原基、花、种子、果实、核、谷粒、细胞或其部分。在一些实施方式中,部分是果实、核或谷粒。 在一些方面中,本公开涉及任何以上实施方式的基因改变的植物的花粉粒或胚珠。 在一些方面中,本公开涉及从任何以上实施方式的植物产生的原生质体。 在一些方面中,本公开涉及从来自任何以上实施方式的植物的原生质体或细胞产生的组织培养物,其中细胞或原生质体从选自由下述组成的组中的植物部分产生:叶、花药、雌蕊、茎、叶柄、根、根原基、根尖、果实、种子、花、子叶、下胚轴、胚胎和分生组织细胞。 本公开的某些方面涉及产生任何以上实施方式的基因改变的植物的方法,其包括将基因改变引入包括核酸序列的植物。在一些实施方式中,核酸序列可操作地连接至启动子。在一些实施方式中,启动子是根特异性启动子。在一些实施方式中,启动子选自下述的组中:NFR1或NFR5/NFP启动子、莲属NFR5启动子(SEQ ID NO:24)和莲属NFR1启动子(SEQ IDNO:25)、玉蜀黍甲硫氨酸启动子、几丁质酶启动子、玉蜀黍ZRP2启动子、番茄LeExtl启动子、谷氨酰胺合酶大豆根启动子、RCC3启动子、水稻antiquitine启动子、LRR受体激酶启动子或拟南芥pCO2启动子。在一些实施方式中,启动子是任选地选自下述的组中的组成型启动子:CaMV35S启动子、CaMV35S启动子的衍生物、玉蜀黍泛素启动子、三叶启动子、静脉花叶木薯病毒启动子或拟南芥UBQ10启动子。在一些实施方式中,核酸序列被插入植物的基因组,以便核酸序列可操作地连接至内源启动子。在一些实施方式中,内源启动子是根特异性启动子。 在一些方面中,本公开涉及含有编码异源受体多肽的核酸序列的基因改变的植物种子,其中异源受体多肽选自下述的组中:与SEQ ID NO:5[鹰嘴豆/鹰嘴豆(Cicerarietinum)NFR5]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第一多肽,与SEQ IDNO:7[菜豆/菜豆(Phaseolus vulgaris)NFR5]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第二多肽,与SEQ ID NO:9[花生/花生属(Arachis)NFR5]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第三多肽,与SEQ ID NO:11[莲属(Lotus)LYS11]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第四多肽,与SEQ ID NO:12[苜蓿属(Medicago)LYR1]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第五多肽,与SEQ ID NO:13[拟山黄麻属(Parasponia)NFP1]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第六多肽,与SEQID NO:16[大麦HvLysM-RLK1(AK370300)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第七多肽,与SEQ ID NO:17[大麦HvLysM-RLK2(AK357612)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第八多肽,与SEQ ID NO:18[大麦HvLysM-RLK3 AK372128]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第九多肽,与SEQ ID NO:19[大麦HvLysM-RLK10(HORVU4Hr1G066170)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十多肽,与SEQID NO:20[玉蜀黍ZM1(XP_020399958)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十一多肽,与SEQ ID NO:21[玉蜀黍ZM5(XP_008652982.1)]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十二多肽,与SEQ ID NO:22[苹果NFP5 XP_008338966.1]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十三多肽或与SEQ ID NO:23[草莓NFR5 XP_004300586.2]具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性、至少95%序列同一性、至少96%序列同一性、至少97%序列同一性、至少98%序列同一性或至少99%序列同一性的第十四多肽。在一些实施方式中,异源受体多肽选自下述的组中:SEQ ID NO:5[鹰嘴豆/鹰嘴豆(Cicer arietinum)NFR5]、SEQ ID NO:7[菜豆/菜豆(Phaseolus vulgaris)NFR5]、SEQ ID NO:9[花生/花生属(Arachis)NFR5]、SEQ ID NO:11[莲属(Lotus)LYS11]、SEQ ID NO:12[苜蓿属(Medicago)LYR1]、SEQ ID NO:13[拟山黄麻属(Parasponia)NFP1]、SEQ ID NO:16[大麦HvLysM-RLK1(AK370300)]、SEQ ID NO:17[大麦HvLysM-RLK2(AK357612)]、SEQ ID NO:18[大麦HvLysM-RLK3AK372128]、SEQ ID NO:19[大麦HvLysM-RLK10(HORVU4Hr1G066170)]、SEQ ID NO:20[玉蜀黍ZM1(XP_020399958)]、SEQ ID NO:21[玉蜀黍ZM5(XP_008652982.1)]、SEQ ID NO:22[苹果NFP5 XP_008338966.1]或SEQ ID NO:23[草莓NFR5 XP_004300586.2]。在一些实施方式中,异源受体多肽的表达允许植物或其部分通过异源受体多肽识别脂壳寡糖(LCO)。在一些实施方式中,LCO由固氮菌或由菌根真菌产生。在一些实施方式中,LCO由选自下述的组中的固氮菌产生:百脉根中慢生根瘤菌(Mesorhizobium loti)、华癸中慢生根瘤菌(Mesorhizobium huakuii)、地中海中慢生根瘤菌(Mesorhizobium mediterraneum)、鹰嘴豆中慢生根瘤菌(Mesorhizobium ciceri)、中慢生根瘤菌属(Mesorhizobium)物种、蒙古根瘤菌(Rhizobium mongolense)、热带根瘤菌(Rhizobium tropici)、菜豆埃特里根瘤菌(Rhizobium etli phaseoli)、贾氏根瘤菌(Rhizobium giardinii)、豌豆根瘤菌(Rhizobium leguminosarum)任选地三叶草豌豆根瘤菌(R.leguminosarum trifolii)、蚕豆豌豆根瘤菌(R.leguminosarum viciae)和菜豆豌豆根瘤菌(R.leguminosarum phaseoli)、伯克氏菌目(Burkholderiales)任选地含羞草的共生体、苜蓿中华根瘤菌(Sinorhizobium meliloti)、药用中华根瘤菌(Sinorhizobiummedicae)、费氏中华根瘤菌(Sinorhizobium fredii)、费氏中华根瘤菌(Sinorhizobiumfredii)NGR234、茎瘤固氮根瘤菌(Azorhizobium caulinodans)、大豆慢生根瘤菌(Bradyrhizobium japonicum)、埃氏慢生根瘤菌(Bradyrhizobium elkanii)、辽参慢生根瘤菌(Bradyrhizobium liaonginense)、根瘤菌属(Rhizobium)物种、中慢生根瘤菌属(Mesorhizobium)物种、中华根瘤菌属(Sinorhizobium)物种、固氮根瘤菌属(Azorhizobium)物种、弗兰克氏菌属(Frankia)物种或其任何组合,或由选自下述的组中的菌根真菌产生:无柄孢子科(Acaulosporaceae)物种、多孢囊霉科(Diversisporaceae)物种、巨孢囊霉科(Gigasporaceae)物种、平囊霉科(Pacisporaceae)物种、管柄囊霉属(Funneliformis)物种、球囊霉属(Glomus)物种、球囊霉属(Rhizophagus)物种、硬囊霉属(Sclerocystis)物种、Septoglomus属物种、Claroideoglomus属物种、Ambispora属物种、原囊霉属(Archaeospora)物种、Geosiphon pyriformis属物种、类球囊霉属(Paraglomus)属物种、球囊霉门(Glomeromycota)中的其他物种或其任何组合。在一些实施方式中,当种子生长为植物时,异源多肽位于植物细胞质膜。在一些实施方式中,植物细胞是根细胞。在一些实施方式中,根细胞是根表皮细胞或根皮质细胞。在一些实施方式中,当种子生长为植物时,异源多肽在发育中的植物根系中表达。在一些实施方式中,核酸序列可操作地连接至启动子。在一些实施方式中,启动子是根特异性启动子。在一些实施方式中,启动子选自下述的组中:NFR1或NFR5/NFP启动子、莲属NFR5启动子(SEQ ID NO:24)和莲属NFR1启动子(SEQID NO:25)、玉蜀黍甲硫氨酸启动子、几丁质酶启动子、玉蜀黍ZRP2启动子、番茄LeExtl启动子、谷氨酰胺合酶大豆根启动子、RCC3启动子、水稻antiquitine启动子、LRR受体激酶启动子或拟南芥pCO2启动子。在一些实施方式中,启动子是任选地选自下述的组中的组成型启动子:CaMV35S启动子、CaMV35S启动子的衍生物、玉蜀黍泛素启动子、三叶启动子、静脉花叶木薯病毒启动子或拟南芥UBQ10启动子。在一些实施方式中,植物选自下述的组中:玉米(例如,玉蜀黍、玉米(Zea mays)),水稻(例如,亚洲栽培稻(Oryza sativa)、非洲栽培稻(Oryzaglaberrima)、菰属(Zizania)物种,大麦(例如,大麦(Hordeum vulgare),小麦(例如,普通小麦、斯卑尔脱小麦、硬粒小麦、普通小麦(Triticum aestivum)、斯卑尔脱小麦(Triticumspelta)、硬粒小麦(Triticum durum)、小麦属(Triticum)物种),山黄麻属(Trema)物种(例如,光叶山黄麻(Trema cannabina)、Trema cubense、Trema discolor、Tremadomingensis、Trema integerrima、Trema lamarckiana、小花山黄麻(Trema micrantha)、异色山黄麻(Trema orientalis)、Trema philippinensis、Trema strigilosa、山黄麻(Trema tomentosa)),苹果(例如,西洋苹果(Malus pumila),梨(例如,西洋梨(Pyruscommunis)、白梨(Pyrus×bretschneideri)、沙梨(Pyrus pyrifolia)、新疆梨(Pyrussinkiangensis)、川梨(Pyrus pashia)、梨属(Pyrus)物种),李子(例如,西梅、西洋李子,布拉斯李、欧洲李(Prunus domestica)、中国李(Prunus salicina)),杏子(例如,山杏(Prunus armeniaca)、布里扬松杏(Prunus brigantina)、东北杏(Prunus mandshurica)、梅(Prunus mume)、西伯利亚杏(Prunus sibirica)),桃子(例如,油桃、桃(Prunuspersica),扁桃(例如,甜扁桃(Prunus dulcis)、扁桃(Prunus amygdalus)),胡桃(例如,波斯胡桃、英国胡桃、黑胡桃、胡桃(Juglans regia)、黑胡桃(Juglans nigra)、白胡桃(Juglans cinerea)、加州胡桃(Juglans californica)),草莓(例如,草莓(Fragaria×ananassa)、智利草莓(Fragaria chiloensis)、弗州草莓(Fragaria virginiana)、野草莓(Fragaria vesca)),树莓(例如,欧洲红树莓、黑树莓、覆盆子(Rubus idaeus)、美国黑树莓(Rubus occidentalis)、硬毛树莓(Rubus strigosus)),黑莓(例如,常青黑莓,喜马拉雅黑莓、黑莓(Rubus fruticosus)、北美黑莓(Rubus ursinus)、裂叶黑莓(Rubus laciniatus)、尖齿黑莓(Rubus argutus)、亚美尼亚黑莓(Rubus armeniacus)、露莓(Rubus plicatus)、榆叶黑莓(Rubus ulmifolius)、美国黑莓(Rubus allegheniensis)),红醋栗(例如,红丛茶蔍子(Ribes rubrum)、红茶藨子(Ribes spicatum)、高山茶藨子(Ribesbes alpinum)、Ribes schlechtendalii、多花茶藨子(Ribes multiflorum)、岩生茶藨子(Ribespetraeum)、伏生茶藨子(Ribes triste)),黑醋栗(例如,黑茶藨子(Ribes nigrum),甜瓜(例如,西瓜、冬瓜、卡萨巴甜瓜、哈密瓜、白兰瓜、香瓜、西瓜(Citrullus lanatus)、冬瓜(Benincasa hispida)、罗马甜瓜(Cucumis melo cantalupensis)、冬甜瓜(Cucumis meloinodorus)、网纹甜瓜(Cucumis melo reticulatus)),黄瓜(例如,切黄瓜、腌制黄瓜、英国黄瓜、黄瓜(Cucumis sativus)),南瓜(例如,美国南瓜(Cucurbita pepo)、笋瓜(Cucurbitamaxima)),倭瓜(例如,葫芦、西葫芦(Cucurbita argyrosperma)、黑子南瓜(Cucurbitaficifolia)、笋瓜(Cucurbita maxima)、中国南瓜(Cucurbita moschata)),葡萄(例如,葡萄(Vitis vinifera)、山葡萄(Vitis amurensis)、美洲葡萄(Vitis labrusca)、白亮葡萄(Vitis mustangensis)、河岸葡萄(Vitis riparia)、圆叶葡萄(Vitis rotundifolia))或大麻(例如,印度大麻、大麻(Cannabis sativa))。 在一些方面中,本公开涉及从以上实施方式的任何一项的基因改变的植物种子产生的植物,其中植物表达异源多肽,并且其中异源多肽的表达允许植物通过异源受体多肽识别脂壳寡糖(LCO)。 附图说明 图1A-1B显示NFP受体胞外域(NFP-ECD)的结构。图1A显示具有标记的三个LysM结构域(LysM1、LysM2和LysM3)的NFP-ECD。LysM结构域内的基序也被标记:LysM1基序=α1、α2、β1和β2;LysM2基序=α3、α4、β3和β4;和LysM3基序=α5、α6、β5和β6。显示了糖基化(二GlcNAc核心(上方处来自α1的突出;邻近β2和β1的中心处以及在α4的左下方处可见的另外核心),并且二硫桥用箭头指示且用残基数标记(C47-C166、C39-C104和C102-C164)。图1B显示示出受体的刚性柄区域的NFP-ECD的SAXS包膜。整体尺寸以埃 图2A-2B显示使用苜蓿中华根瘤菌LCO-IV和LCO-V以及百脉根中慢生根瘤菌LCO-V和CO6的生物层干涉法(BLI)结合曲线。图2A显示NFP以26±0.2μM的平均K 图3A-3B显示苜蓿中华根瘤菌LCO-IV突变体,和使用这些变体的结合测定的结果。图3A显示具有箭头的苜蓿中华根瘤菌LCO-IV突变体的示意图,箭头指示四个突变NodL、NodH、NodFE和NodFL的每一个在LCO-IV中影响的位置。图3B显示使用三LCO-IV突变体并且苜蓿中华根瘤菌LCO-IV作为对照进行的结合测定。LCO-IV显示的结果来自七次重复、NodH(苜蓿中华根瘤菌ΔH)显示的结果来自四次重复、NodFE(苜蓿中华根瘤菌ΔFE)显示的结果来自三次重复和NodFL(苜蓿中华根瘤菌ΔFL)显示的结果来自三次重复。 图4A-4B显示苜蓿属NFP LysM2结构域中的疏水补丁以及使用疏水补丁内重要残基的突变体的结合测定测量。图4A显示将CO4(命名为“配体”)分子对接至用静电表面电势遮蔽住的苜蓿属NFP。由黑色虚线圈出疏水补丁,且使用箭头显示了重要残基L147和L154的位置。用灰色虚线描绘了LCO脂肪酸的位置。图4B显示比较野生型(WT)NFP(“NFP WT”)与在残基147和154(“NFP L147D L154D”;粗体)处突变的NFP的结合测定测量。NFP WT显示的结果来自七次重复和NFP L147D L154D显示的结果来自四次重复。 图5显示用于突变体互补实验的构建体的总体示意图。命名如下:T-DNA左边界序列=LB,T-DNA右边界序列=RB,β-葡糖醛酸酶基因=GUS,缓冲区序列=缓冲剂,早期结瘤素-11前体启动子=pEnod11,NFP启动子=pNfp。箭头指示基因转录的方向。 图6A-6B显示苜蓿属nfp突变体的互补试验。图6A显示用苜蓿中华根瘤菌菌株2011接种所测试的互补;柱表示49dpi后的平均结节数。图6B显示用药用中华根瘤菌接种测试的互补;柱表示28dpi后的平均结节数。对于图6A-6B,圆圈表示个体计数;空的圆圈=苜蓿Jemalong野生型背景(WT);实心圆圈=苜蓿属nfp突变体背景(nfp);EVC=空的载体对照;WT=野生型NFP对照;误差线显示SEM;不同字母指示样品之间的显著差异(ANOVA,Tukey,P 图7A-7G显示其他LCO受体胞外域的同源性建模,具有根据其静电电势遮蔽住的不同LCO受体的表面描绘。当存在时,由黑色虚线圈出疏水补丁和由灰色虚线圈出阴性补丁。对接的配体(几丁质)作为指导显示。图7A显示其他表征的LCO受体:莲属NFR5、豌豆(豌豆(Pisum sativum))SYM10和大豆(大豆(Glycine max))NFR5α的同源性建模。图7B显示具有疏水补丁的鹰嘴豆(鹰嘴豆(Cicer arietinum))NFR5、菜豆(菜豆(Phaseolus vulgaris))NFR5和花生(花生(Arachis hypogaea))NFR5中的之前未表征的LCO受体同系物的同源性建模。图7C-7E显示更远缘相关受体的同源性建模,包括(图7C)LYS受体:LYS11、LYS12、LYS13、LYS14、LYS15、LYS16、LYS17和LYS18;(图7D)LYR受体:LYR1、LYR2、LYR3和LYR4以及(图7E)NFP受体:拟山黄麻(Parasponia)NFP1和拟山黄麻(Parasponia)NFP2。图7F显示不具有疏水补丁的苜蓿属LYR3和莲属LYS12受体的模型(从LysM3结构域和具有对接的配体(几丁质)观察的模型)。图7G显示莲属LYS11模型(LYS11–模型;左;还在图7C中)与莲属LYS11的晶体结构(LYS11–晶体结构;右)的比较。 图8A-8C显示从以下选择的LysM受体的比对:拟南芥(At;AT3G21630_CERK1(SEQID NO:37)、AT1G77630_LYP3(SEQ ID NO:42)、AT2G17120_LYP1(SEQ ID NO:44))、玉米(Zeamays)(Zm;ZM9_NP_001146346.1(SEQ ID NO:34))、大麦(Hordeum vulgare)(Hv;HvLysMRLK4_AK369594.1(SEQ ID NO:35))、蒺藜苜蓿(Medicago truncatula)(Mt或Medtr;Mt_LYK9_XP_003601376(SEQ ID NO:31)、Mt_LYK3_XP_003616958(SEQ ID NO:33)、Mt_LYK10_XP_003613165(SEQ ID NO:39)、Medtr5g042440.1(SEQ ID NO:41))、亚洲栽培稻(Oryza sativa)(Os;XP_015611967_OsCERK1(SEQ ID NO:36)、OsCeBiP(SEQ ID NO:43))和百脉根(Lotus japonicus)(Lj;BAI79273.1_CERK6(SEQ ID NO:30)、CAE02590.1_NFR1(SEQID NO:32)、BAI79284.1_EPR3(SEQ ID NO:38)、CAE02597.1_NFR5(SEQ ID NO:40))。NFR1和NFR5是Nod因子受体,EPR3是胞外多糖受体,AtLYP1和AtLYP3是肽聚糖受体,AtCERK1、OsCERK1、OsCeBIP、CERK6是壳寡糖受体。显示了三个LysM结构域的侧面的C(x)XXXC和CxC基序。显示了LysM1(黑线)、LysM2(灰线)和LysM3(灰线)。图8A显示包括全部LysM1结构域和部分LysM2结构域的比对的前两个部分。图8B显示包括剩余LysM2结构域和全部LysM3结构域的比对的第三和第四部分。图8C显示比对的第五部分。 图9A-9B显示从以下选择的LysM受体的比对:拟南芥(At;AT3G21630_CERK1(SEQID NO:37))、玉米(Zea mays)(Zm;XP_020399958_ZM1(SEQ ID NO:20)、XP_008652982.l_ZM5(SEQ ID NO:21)、AQK73561.1_ZM7(SEQ ID NO:46)、NP_001147981.1_ZM3(SEQ ID NO:47)、NP_001147941.2_ZM6(SEQ ID NO:48)、AQK58792.1_ZM4(SEQ ID NO:49)、ZM9_NP_001146346.1(SEQ ID NO:34))、大麦(Hordeum vulgare)(Hv;HORVU4Hr1G066170_HvLysMRLK10(SEQ ID NO:19)、AK357612_HvLysMRLK2(SEQ ID NO:17)、AK370300_HvLysmRLK1(SEQ ID NO:16)、AK372128_HvLysMRLK3(SEQ ID NO:18)、HvLysMRLK4_AK369594.1(SEQ ID NO:35))、亚洲栽培稻(Oryza sativa)(Os;XP_015611967_OsCERK1(SEQ ID NO:36))、蒺藜苜蓿(Medicago truncatula)(Mt;XP_003613904.2_MtNFP(SEQ IDNO:45)、Mt_LYK3_XP_003616958(SEQ ID NO:33)、Mt_LYK9_XP_003601376(SEQ ID NO:31))和百脉根(Lotus japonicus)(Lj;CAE02590.1_NFR1(SEQ ID NO:32)、CAE02597.1_NFR5(SEQ ID NO:40)、BAI79273.1_CERK6(SEQ ID NO:30))。LjNFR1、LjNFR5、MtLYK3和MtNFP是功能Nod因子受体,AtCERK1、OsCERK1、LjCERK6是功能几丁质受体。显示了三个LysM结构域的侧面的C(x)XXXC和CxC基序。显示了LysM1(黑线)、LysM2(灰线)和LysM3(灰线)。位于LysM1之前的C(x)XXXC基序中“X”残基的数量在受体之间变化,因此在该图中和在连续的图中的比对中,LysM1的位置(黑线)相应地变化。图9A显示包括全部LysM1结构域和部分LysM2结构域的比对的第一和第二部分。图9B显示包括剩余LysM2结构域和全部LysM3结构域的比对的第三和第四部分。 图10A-10B显示从以下选择的LysM受体的比对:玉米(Zea mays)(Zm;ONM41523.1_ZM8(SEQ ID NO:50)、XP_008657477.1_ZM2(SEQ ID NO:51)、Zm00001d043516ZM10(SEQ IDNO:53))、大麦(Hordeum vulgare)(Hv;MLOC_5489.2_HvLysM-RLK9(SEQ ID NO:52)、MLOC_18610.1_HvLysM-RLK8(SEQ ID NO:54)、MLOC_57536.1_HvLysM-RLK6(SEQ ID NO:55))、蒺藜苜蓿(Medicago truncatula)(Mt;Mt_LYK10_XP_003613165(SEQ ID NO:39)、Mt_LYK3_XP_003616958(SEQ ID NO:33)、XP_003613904.2_MtNFP(SEQ ID NO:45))和百脉根(Lotusjaponicus)(Lj;BAI79284.1_EPR3(SEQ ID NO:38),CAE02590.1_NFR1(SEQ ID NO:32),CAE02597.1_NFR5(SEQ ID NO:40))。LjNFR1、LjNFR5、MtLYK3和MtNFP是功能Nod因子受体,LjEPR3是功能EPS受体。显示了三个LysM结构域的侧面的C(x)XXXC和CxC基序。显示了LysM1(黑线)、LysM2(灰线)和LysM3(灰线)。图10A显示包括全部LysM1结构域和全部LysM2结构域的比对的第一、第二和第三部分。图10B显示包括全部LysM3结构域的比对的第四、第五和第六部分。 图11A-11B显示从以下选择的LysM受体的比对:拟南芥(At;AT1G21880.2_LYP2(SEQ ID NO:56)、AT1G77630_LYP3(SEQ ID NO:42)、AT2G17120_LYP1(SEQ ID NO:44))、亚洲栽培稻(Oryza sativa)(Os;OsCeBiP(SEQ ID NO:43))和百脉根(Lotus japonicus)(Lj;LjLYP1(SEQ ID NO:57)、LjLYP2(SEQ ID NO:58)、LjLYP3(SEQ ID NO:58)、CAE02590.1_NFR1(SEQ ID NO:32)、CAE02597.1_NFR5(SEQ ID NO:40))。LjNFR1、LjNFR5是功能Nod因子受体,AtLYP2和AtLYP3是PGN受体,OsCeBiP是功能几丁质受体。显示了三个LysM结构域的侧面的C(x)XXXC和CxC基序。显示了LysM1(黑线),LysM2(灰线)和LysM3(灰线)。图11A显示包括全部LysM1结构域、全部LysM2结构域和全部LysM3结构域的比对的第一、第二、第三和第四部分。图11B显示比对的第五、第六和第七部分。 图12A-12E显示先前已知的LCO受体和新鉴定的LCO受体的注释的氨基酸序列。图12A显示了注释关键;用下划虚线显示LysM1结构域,用下划实线显示LysM2结构域,以粗体显示疏水补丁残基和用斜体的残基显示LysM3结构域。显示了苜蓿属NFP(MtNFP/1-595;SEQID NO:1)、莲属NFR5(已知的LCO受体;LjNFR5/1-595;SEQ ID NO:2)、豌豆SYM10(已知的LCO受体;豌豆_SYM10/1-594;SEQ ID NO:3)和大豆NFR5α(已知的LCO受体;GmNFR5α/1-598max;SEQ ID NO:4)。图12B显示了鹰嘴豆NFR5(新的LCO受体;鹰嘴豆NFR5/1-557(鹰嘴豆(Cicerarietinum));SEQ ID NO:5)、菜豆NFR5(新的LCO受体;BeanNFR5/1-597(菜豆(Phaseolusvulgaris));SEQ ID NO:7)、花生NFR5(新的LCO受体;PeanutNFR5/1-595[花生亚种花生(Arachis hypogaea subsp.hypogaea)];SEQ ID NO:9)和莲属LYS11(新的LCO受体;LjLys11/1-591;SEQ ID NO:11)。图12C显示了苜蓿属LYR1(新的LCO受体;MtLYR1/1-590;SEQ ID NO:12)、拟山黄麻属NFP1(新的LCO受体;PanNFP1/1-613;SEQ ID NO:13)、拟山黄麻属NFP2(已知的LCO受体;PanNFP2/1-582;SEQ ID NO:14)和大麦受体HvLysM-RLK1(新的LCO受体;HvLysM-RLK1(AK370300);SEQ ID NO:16)。图12D显示了大麦受体HvLysM-RLK2(新的LCO受体;HvLysM-RLK2(AK357612);SEQ ID NO:17)、大麦受体HvLysM-RLK3 AK372128(新的LCO受体;HvLysM-RLK3AK372128;SEQ ID NO:18)、大麦受体HvLysM-RLK10(新的LCO受体;HvLysM-RLK10(HORVU4Hr1G066170);SEQ ID NO:19)和玉蜀黍受体ZM1(新的LCO受体;ZM1(XP_020399958);SEQ ID NO:20)。图12E显示了玉蜀黍受体ZM5(新的LCO受体;ZM5(XP_008652982.1);SEQ ID NO:21)、苹果NFP5(新的LCO受体;XP_008338966.1PREDICTED:丝氨酸/苏氨酸受体样激酶NFP[苹果(Malus domestica)];SEQ ID NO:22)和草莓NFR5(新的LCO受体;XP_004300586.2PREDICTED:蛋白质LYK5样[野草莓亚种草莓(Fragaria vescasubsp.vesca)];SEQ ID NO:23)。 图13A-13D显示了大麦RLK10受体(HvRLK10)胞外域的同源性建模和使用HvRLK10胞外域的结合实验的结果。图13A在顶部显示了纯化的HvRLK10胞外域的示意图(N-末端=N’;LysM1=M1;LysM2=M2;LysM3=M3;用于纯化的6x组氨酸标签=6xHIS;C-末端=C’)和在底部显示了具有CO5的HvRLK10胞外域的结合测定的结果。图13B显示了大麦受体RLK10(HvRLK10)胞外域的同源性建模,具有根据其静电电势遮蔽住的表面描绘。由黑色虚线圈出疏水补丁,且疏水补丁的顶部处显示了CO配体。图13C显示了具有百脉根中慢生根瘤菌LCO的HvRLK10胞外域的结合测定的结果。图13D显示了具有苜蓿中华根瘤菌LCO的HvRLK10胞外域的结合测定的结果。对于图13A和13C-13D,y-轴上显示了以nm的结合、x-轴上显示了以秒(s)的时间和图的标题中显示了测试的分子(CO5,百脉根中慢生根瘤菌LCO和苜蓿中华根瘤菌LCO)。 图14A-14C显示了去糖基化的NFP-ECD和糖基化的NFP-ECD的SAXS分析和用于去糖基化的NFP-ECD和糖基化的NFP-ECD的无量纲的Kratky图。图14A显示了SAXS分析,示出对于去糖基化的NFP-ECD的具有模型拟合的散射曲线(c2;左图),纪尼厄图(Guinier plot)(顶部中间图),和具有指示的Dmax的P(r)距离分布图(底部中间图),以及对于去糖基化的NFP-ECD,对接入SAXS包膜的NFP-ECD晶体结构(显示了延伸茎样结构;整体尺寸以埃 图15A-15E显示了NFP-ECD结合至苜蓿中华根瘤菌LCO-V、百脉根中慢生根瘤菌LCO-V和几丁质(壳五糖;CO5)的BLI结合曲线。图15A显示了NFP-ECD以22.3±0.1μM的平均K 图16A-16D显示了WT NFP-ECD和疏水补丁突变体NFP-ECD(L147D/L154D)结合至苜蓿中华根瘤菌LCO-IV的BLI结合曲线和NFP受体的示意图。图16A显示了WT NFP-ECD结合至苜蓿中华根瘤菌LCO-IV。图16B显示了L147D/L154D NFP-ECD结合至苜蓿中华根瘤菌LCO-IV。对于图16A-16B,将七个2倍稀释系列的分析物(1.56–100μM)用于每个实验;和以实线代表实验结合曲线,以虚线代表拟合曲线。图16C显示了总结图16A-16B的动力学参数的表,具有拟合优度通过每个点的平均值的整体拟合R 图17A-17B显示了关于拟南芥CERK1(AtCERK1)结合至壳五糖(CO5)和壳八糖(CO8)的BLI结合曲线。图17A显示了AtCERK1结合至壳五糖(几丁质(CO5))。图17B显示了AtCERK1结合至壳八糖(几丁质(CO8))。关于图17A-17B示出了将七个2倍稀释系列的分析物(1.56–100μM)用于每个实验;以实线代表实验结合曲线,以虚线代表拟合曲线;拟合优度通过每个点的平均值的整体拟合R 具体实施方式 以下描述陈述示例性方法、参数等。然而,应该认识到这种描述不旨在作为本公开的范围的限制,而是作为示例性实施方式的描述提供的。
本公开的某些方面涉及含有编码异源受体多肽的核酸序列的基因改变的植物或植物部分,其中异源受体多肽选自下述的组中:与SEQ ID NO:5(即鹰嘴豆,鹰嘴豆(Cicerarietinum)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第一多肽,与SEQ ID NO:7(即菜豆,菜豆(Phaseolus vulgaris)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第二多肽,与SEQ ID NO:9(即花生,花生属(Arachis)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第三多肽,与SEQ ID NO:11(即莲属(Lotus)LYS11)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第四多肽,与SEQ ID NO:12(即苜蓿属LYR1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第五多肽,与SEQ ID NO:13(即拟山黄麻属NFP1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第六多肽,与SEQ ID NO:16(即大麦HvLysM-RLK1(AK370300))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第七多肽,与SEQ ID NO:17(即大麦HvLysM-RLK2(AK357612))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第八多肽,与SEQ ID NO:18(即大麦HvLysM-RLK3 AK372128)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第九多肽,与SEQID NO:19(即大麦HvLysM-RLK10(HORVU4Hr1G066170))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十多肽,与SEQ ID NO:20(即玉蜀黍ZM1(XP_020399958))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十一多肽,与SEQ ID NO:21(即玉蜀黍ZM5(XP_008652982.1))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十二多肽,与SEQ ID NO:22(即苹果NFP5 XP_008338966.1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十三多肽或与SEQ ID NO:23(即草莓NFR5 XP_004300586.2)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十四多肽。在一些实施方式中,异源受体多肽选自下述的组中:SEQ ID NO:5(即鹰嘴豆,鹰嘴豆(Cicerarietinum)NFR5)、SEQ ID NO:7(即菜豆,菜豆(Phaseolus vulgaris)NFR5)、SEQ ID NO:9(即花生,花生属(Arachis)NFR5)、SEQ ID NO:11(即莲属(Lotus)LYS11)、SEQ ID NO:12(即苜蓿属(Medicago)LYR1)、SEQ ID NO:13(即拟山黄麻属(Parasponia)NFP1)、SEQ ID NO:16(即大麦HvLysM-RLK1(AK370300))、SEQ ID NO:17(即大麦HvLysM-RLK2(AK357612))、SEQID NO:18(即大麦HvLysM-RLK3AK372128)、SEQ ID NO:19(即大麦HvLysM-RLK10(HORVU4Hr1G066170))、SEQ ID NO:20(即玉蜀黍ZM1(XP_020399958))、SEQ ID NO:21(即玉蜀黍ZM5(XP_008652982.1))、SEQ ID NO:22(即苹果NFP5 XP_008338966.1)和SEQ ID NO:23(即草莓NFR5 XP_004300586.2)。在一些实施方式中,异源受体多肽的表达允许植物或其部分通过异源受体多肽识别脂壳寡糖(LCO)。在一些实施方式中,LCO由固氮菌或由菌根真菌产生。在一些实施方式中,LCO由选自下述的组中的固氮菌产生:百脉根中慢生根瘤菌(Mesorhizobium loti)、华癸中慢生根瘤菌(Mesorhizobium huakuii)、地中海中慢生根瘤菌(Mesorhizobium mediterraneum)、鹰嘴豆中慢生根瘤菌(Mesorhizobium ciceri)、中慢生根瘤菌属(Mesorhizobium)物种、蒙古根瘤菌(Rhizobium mongolense)、热带根瘤菌(Rhizobium tropici)、菜豆埃特里根瘤菌(Rhizobium etli phaseoli)、贾氏根瘤菌(Rhizobium giardinii)、豌豆根瘤菌(Rhizobium leguminosarum)任选地三叶草豌豆根瘤菌(R.leguminosarum trifolii)、蚕豆豌豆根瘤菌(R.leguminosarum viciae)和菜豆豌豆根瘤菌(R.leguminosarum phaseoli)、伯克氏菌目(Burkholderiales)任选地含羞草的共生体、苜蓿中华根瘤菌(Sinorhizobium meliloti)、药用中华根瘤菌(Sinorhizobiummedicae)、费氏中华根瘤菌(Sinorhizobium fredii)、费氏中华根瘤菌(Sinorhizobiumfredii)NGR234、茎瘤固氮根瘤菌(Azorhizobium caulinodans)、大豆慢生根瘤菌(Bradyrhizobium japonicum)、埃氏慢生根瘤菌(Bradyrhizobium elkanii)、辽参慢生根瘤菌(Bradyrhizobium liaonginense)、根瘤菌属(Rhizobium)物种、中慢生根瘤菌属(Mesorhizobium)物种、中华根瘤菌属(Sinorhizobium)物种、固氮根瘤菌属(Azorhizobium)物种、弗兰克氏菌属(Frankia)物种或其任何组合,或由选自下述的组中的菌根真菌产生:无柄孢子科(Acaulosporaceae)物种、多孢囊霉科(Diversisporaceae)物种、巨孢囊霉科(Gigasporaceae)物种、平囊霉科(Pacisporaceae)物种、管柄囊霉属(Funneliformis)物种、球囊霉属(Glomus)物种、球囊霉属(Rhizophagus)物种、硬囊霉属(Sclerocystis)物种、Septoglomus属物种、Claroideoglomus属物种、Ambispora属物种、原囊霉属(Archaeospora)物种、Geosiphon pyriformis属物种、类球囊霉属(Paraglomus)属物种、球囊霉门(Glomeromycota)中的其他物种或其任何组合。在一些实施方式中,异源多肽位于植物细胞质膜。在一些实施方式中,植物细胞是根细胞。在一些实施方式中,根细胞是根表皮细胞或根皮质细胞。在一些实施方式中,异源多肽在发育中的植物根系中表达。在一些实施方式中,核酸序列可操作地连接至启动子。在一些实施方式中,启动子是根特异性启动子。在一些实施方式中,启动子选自下述的组中:NFR1或NFR5/NFP启动子、莲属NFR5启动子(SEQ ID NO:24)和莲属NFR1启动子(SEQ ID NO:25)、玉蜀黍甲硫氨酸启动子、几丁质酶启动子、玉蜀黍ZRP2启动子、番茄LeExtl启动子、谷氨酰胺合酶大豆根启动子、RCC3启动子、水稻antiquitine启动子、LRR受体激酶启动子或拟南芥pCO2启动子。在一些实施方式中,启动子是任选地选自下述的组中的组成型启动子:CaMV35S启动子、CaMV35S启动子的衍生物、玉蜀黍泛素启动子、三叶启动子、静脉花叶木薯病毒启动子或拟南芥UBQ10启动子。在一些实施方式中,植物选自下述的组中:玉米(例如,玉蜀黍、玉米(Zea mays)),水稻(例如,亚洲栽培稻(Oryza sativa)、非洲栽培稻(Oryza glaberrima)、菰属(Zizania)物种,大麦(例如,大麦(Hordeum vulgare),小麦(例如,普通小麦、斯卑尔脱小麦、硬粒小麦、普通小麦(Triticum aestivum)、斯卑尔脱小麦(Triticum spelta)、硬粒小麦(Triticum durum)、小麦属(Triticum)物种),山黄麻属(Trema)物种(例如,光叶山黄麻(Trema cannabina)、Trema cubense、Trema discolor、Trema domingensis、Trema integerrima、Tremalamarckiana、小花山黄麻(Trema micrantha)、异色山黄麻(Trema orientalis)、Tremaphilippinensis、Trema strigilosa、山黄麻(Trema tomentosa)),苹果(例如,西洋苹果(Malus pumila),梨(例如,西洋梨(Pyrus communis)、白梨(Pyrus×bretschneideri)、沙梨(Pyrus pyrifolia)、新疆梨(Pyrus sinkiangensis)、川梨(Pyrus pashia)、梨属(Pyrus)物种),李子(例如,西梅、西洋李子,布拉斯李、欧洲李(Prunus domestica)、中国李(Prunus salicina)),杏子(例如,山杏(Prunus armeniaca)、布里扬松杏(Prunusbrigantina)、东北杏(Prunus mandshurica)、梅(Prunus mume)、西伯利亚杏(Prunussibirica)),桃子(例如,油桃、桃(Prunus persica),扁桃(例如,甜扁桃(Prunus dulcis)、扁桃(Prunus amygdalus)),胡桃(例如,波斯胡桃、英国胡桃、黑胡桃、胡桃(Juglansregia)、黑胡桃(Juglans nigra)、白胡桃(Juglans cinerea)、加州胡桃(Juglanscalifornica)),草莓(例如,草莓(Fragaria×ananassa)、智利草莓(Fragariachiloensis)、弗州草莓(Fragaria virginiana)、野草莓(Fragaria vesca)),树莓(例如,欧洲红树莓、黑树莓、覆盆子(Rubus idaeus)、美国黑树莓(Rubus occidentalis)、硬毛树莓(Rubus strigosus)),黑莓(例如,常青黑莓,喜马拉雅黑莓、黑莓(Rubus fruticosus)、北美黑莓(Rubus ursinus)、裂叶黑莓(Rubus laciniatus)、尖齿黑莓(Rubus argutus)、亚美尼亚黑莓(Rubus armeniacus)、露莓(Rubus plicatus)、榆叶黑莓(Rubus ulmifolius)、美国黑莓(Rubus allegheniensis)),红醋栗(例如,红丛茶蔍子(Ribes rubrum)、红茶藨子(Ribes spicatum)、高山茶藨子(Ribesbes alpinum)、Ribes schlechtendalii、多花茶藨子(Ribes multiflorum)、岩生茶藨子(Ribes petraeum)、伏生茶藨子(Ribes triste)),黑醋栗(例如,黑茶藨子(Ribes nigrum),甜瓜(例如,西瓜、冬瓜、卡萨巴甜瓜、哈密瓜、白兰瓜、香瓜、西瓜(Citrullus lanatus)、冬瓜(Benincasa hispida)、罗马甜瓜(Cucumis melocantalupensis)、冬甜瓜(Cucumis melo inodorus)、网纹甜瓜(Cucumis meloreticulatus)),黄瓜(例如,切黄瓜、腌制黄瓜、英国黄瓜、黄瓜(Cucumis sativus)),南瓜(例如,美国南瓜(Cucurbita pepo)、笋瓜(Cucurbita maxima)),倭瓜(例如,葫芦、西葫芦(Cucurbita argyrosperma)、黑子南瓜(Cucurbita ficifolia)、笋瓜(Cucurbitamaxima)、中国南瓜(Cucurbita moschata)),葡萄(例如,葡萄(Vitis vinifera)、山葡萄(Vitis amurensis)、美洲葡萄(Vitis labrusca)、白亮葡萄(Vitis mustangensis)、河岸葡萄(Vitis riparia)、圆叶葡萄(Vitis rotundifolia))或大麻(例如,印度大麻、大麻(Cannabis sativa))。 在任何以上实施方式的一些实施方式中:植物部分是叶、茎、根、根原基、花、种子、果实、核、谷粒、细胞或其部分。在一些实施方式中,部分是果实、核或谷粒。 在一些方面中,本公开涉及任何以上实施方式的基因改变的植物的花粉粒或胚珠。 在一些方面中,本公开涉及从任何以上实施方式的植物产生的原生质体。 在一些方面中,本公开涉及从来自任何以上实施方式的植物的原生质体或细胞产生的组织培养物,其中细胞或原生质体从选自由下述组成的组中的植物部分产生:叶、花药、雌蕊、茎、叶柄、根、根原基、根尖、果实、种子、花、子叶、下胚轴、胚胎和分生组织细胞。 在一些方面中,本公开涉及含有编码异源受体多肽的核酸序列的基因改变的植物种子,其中异源受体多肽选自下述的组中:与SEQ ID NO:5(即鹰嘴豆,鹰嘴豆(Cicerarietinum)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第一多肽,与SEQ ID NO:7(即菜豆,菜豆(Phaseolus vulgaris)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第二多肽,与SEQ ID NO:9(即花生,花生属(Arachis)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第三多肽,与SEQ ID NO:11(即莲属(Lotus)LYS11)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第四多肽,与SEQ ID NO:12(即苜蓿属(Medicago)LYR1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第五多肽,与SEQ ID NO:13(即拟山黄麻属(Parasponia)NFP1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第六多肽,与SEQ ID NO:16(即大麦HvLysM-RLK1(AK370300))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第七多肽,与SEQ ID NO:17(即大麦HvLysM-RLK2(AK357612))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第八多肽,与SEQ ID NO:18(即大麦HvLysM-RLK3 AK372128)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第九多肽,与SEQ ID NO:19(即大麦HvLysM-RLK10(HORVU4Hr1G066170))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十多肽,与SEQ ID NO:20(即玉蜀黍ZM1(XP_020399958))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十一多肽,与SEQ ID NO:21(即玉蜀黍ZM5(XP_008652982.1))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十二多肽,与SEQ ID NO:22(即苹果NFP5 XP_008338966.1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十三多肽或与SEQ ID NO:23(即草莓NFR5XP_004300586.2)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十四多肽。在一些实施方式中,异源受体多肽选自下述的组中:SEQ ID NO:5(即鹰嘴豆,鹰嘴豆(Cicer arietinum)NFR5),SEQ ID NO:7(即菜豆,菜豆(Phaseolus vulgaris)NFR5),SEQ ID NO:9(即花生,花生属(Arachis)NFR5),SEQ ID NO:11(即莲属(Lotus)LYS11),SEQ ID NO:12(即苜蓿属(Medicago)LYR1),SEQ ID NO:13(即拟山黄麻属(Parasponia)NFP1),SEQ ID NO:16(即大麦HvLysM-RLK1(AK370300)),SEQ ID NO:17(即大麦HvLysM-RLK2(AK357612)),SEQ ID NO:18(即大麦HvLysM-RLK3AK372128),SEQ ID NO:19(即大麦HvLysM-RLK10(HORVU4Hr1G066170)),SEQ ID NO:20(即玉蜀黍ZM1(XP_020399958)),SEQ ID NO:21(即玉蜀黍ZM5(XP_008652982.1)),SEQ ID NO:22(即苹果NFP5XP_008338966.1),和SEQ ID NO:23(即草莓NFR5 XP_004300586.2)。在一些实施方式中,异源受体多肽的表达允许植物或其部分通过异源受体多肽识别脂壳寡糖(LCO)。在一些实施方式中,LCO由固氮菌或由菌根真菌产生。在一些实施方式中,LCO由选自下述的组中的固氮菌产生:百脉根中慢生根瘤菌(Mesorhizobium loti)、华癸中慢生根瘤菌(Mesorhizobiumhuakuii)、地中海中慢生根瘤菌(Mesorhizobium mediterraneum)、鹰嘴豆中慢生根瘤菌(Mesorhizobium ciceri)、中慢生根瘤菌属(Mesorhizobium)物种、蒙古根瘤菌(Rhizobiummongolense)、热带根瘤菌(Rhizobium tropici)、菜豆埃特里根瘤菌(Rhizobium etliphaseoli)、贾氏根瘤菌(Rhizobium giardinii)、豌豆根瘤菌(Rhizobium leguminosarum)任选地三叶草豌豆根瘤菌(R.leguminosarum trifolii)、蚕豆豌豆根瘤菌(R.leguminosarum viciae)和菜豆豌豆根瘤菌(R.leguminosarum phaseoli)、伯克氏菌目(Burkholderiales)任选地含羞草的共生体、苜蓿中华根瘤菌(Sinorhizobium meliloti)、药用中华根瘤菌(Sinorhizobium medicae)、费氏中华根瘤菌(Sinorhizobium fredii)、费氏中华根瘤菌(Sinorhizobium fredii)NGR234、茎瘤固氮根瘤菌(Azorhizobiumcaulinodans)、大豆慢生根瘤菌(Bradyrhizobium japonicum)、埃氏慢生根瘤菌(Bradyrhizobium elkanii)、辽参慢生根瘤菌(Bradyrhizobium liaonginense)、根瘤菌属(Rhizobium)物种、中慢生根瘤菌属(Mesorhizobium)物种、中华根瘤菌属(Sinorhizobium)物种、固氮根瘤菌属(Azorhizobium)物种、弗兰克氏菌属(Frankia)物种或其任何组合,或由选自下述的组中的菌根真菌产生:无柄孢子科(Acaulosporaceae)物种、多孢囊霉科(Diversisporaceae)物种、巨孢囊霉科(Gigasporaceae)物种、平囊霉科(Pacisporaceae)物种、管柄囊霉属(Funneliformis)物种、球囊霉属(Glomus)物种、球囊霉属(Rhizophagus)物种、硬囊霉属(Sclerocystis)物种、Septoglomus属物种、Claroideoglomus属物种、Ambispora属物种、原囊霉属(Archaeospora)物种、Geosiphon pyriformis属物种、类球囊霉属(Paraglomus)属物种、球囊霉门(Glomeromycota)中的其他物种或其任何组合。在一些实施方式中,当种子生长为植物时,异源多肽位于植物细胞质膜。在一些实施方式中,植物细胞是根细胞。在一些实施方式中,根细胞是根表皮细胞或根皮质细胞。在一些实施方式中,当种子生长为植物时,异源多肽在发育中的植物根系中表达。在一些实施方式中,核酸序列可操作地连接至启动子。在一些实施方式中,启动子是根特异性启动子。在一些实施方式中,启动子选自下述的组中:NFR1或NFR5/NFP启动子、莲属NFR5启动子(SEQ ID NO:24)和莲属NFR1启动子(SEQ ID NO:25)、玉蜀黍甲硫氨酸启动子、几丁质酶启动子、玉蜀黍ZRP2启动子、番茄LeExtl启动子、谷氨酰胺合酶大豆根启动子、RCC3启动子、水稻antiquitine启动子、LRR受体激酶启动子或拟南芥pCO2启动子。在一些实施方式中,启动子是任选地选自下述的组中的组成型启动子:CaMV35S启动子、CaMV35S启动子的衍生物、玉蜀黍泛素启动子、三叶启动子、静脉花叶木薯病毒启动子或拟南芥UBQ10启动子。在一些实施方式中,植物选自下述的组中:玉米(例如,玉蜀黍、玉米(Zea mays)),水稻(例如,亚洲栽培稻(Oryzasativa)、非洲栽培稻(Oryza glaberrima)、菰属(Zizania)物种,大麦(例如,大麦(Hordeumvulgare),小麦(例如,普通小麦、斯卑尔脱小麦、硬粒小麦、普通小麦(Triticumaestivum)、斯卑尔脱小麦(Triticum spelta)、硬粒小麦(Triticum durum)、小麦属(Triticum)物种),山黄麻属(Trema)物种(例如,光叶山黄麻(Trema cannabina)、Tremacubense、Trema discolor、Trema domingensis、Trema integerrima、Trema lamarckiana、小花山黄麻(Trema micrantha)、异色山黄麻(Trema orientalis)、Tremaphilippinensis、Trema strigilosa、山黄麻(Trema tomentosa)),苹果(例如,西洋苹果(Malus pumila),梨(例如,西洋梨(Pyrus communis)、白梨(Pyrus×bretschneideri)、沙梨(Pyrus pyrifolia)、新疆梨(Pyrus sinkiangensis)、川梨(Pyrus pashia)、梨属(Pyrus)物种),李子(例如,西梅、西洋李子,布拉斯李、欧洲李(Prunus domestica)、中国李(Prunus salicina)),杏子(例如,山杏(Prunus armeniaca)、布里扬松杏(Prunusbrigantina)、东北杏(Prunus mandshurica)、梅(Prunus mume)、西伯利亚杏(Prunussibirica)),桃子(例如,油桃、桃(Prunus persica),扁桃(例如,甜扁桃(Prunus dulcis)、扁桃(Prunus amygdalus)),胡桃(例如,波斯胡桃、英国胡桃、黑胡桃、胡桃(Juglansregia)、黑胡桃(Juglans nigra)、白胡桃(Juglans cinerea)、加州胡桃(Juglanscalifornica)),草莓(例如,草莓(Fragaria×ananassa)、智利草莓(Fragariachiloensis)、弗州草莓(Fragaria virginiana)、野草莓(Fragaria vesca)),树莓(例如,欧洲红树莓、黑树莓、覆盆子(Rubus idaeus)、美国黑树莓(Rubus occidentalis)、硬毛树莓(Rubus strigosus)),黑莓(例如,常青黑莓,喜马拉雅黑莓、黑莓(Rubus fruticosus)、北美黑莓(Rubus ursinus)、裂叶黑莓(Rubus laciniatus)、尖齿黑莓(Rubus argutus)、亚美尼亚黑莓(Rubus armeniacus)、露莓(Rubus plicatus)、榆叶黑莓(Rubus ulmifolius)、美国黑莓(Rubus allegheniensis)),红醋栗(例如,红丛茶蔍子(Ribes rubrum)、红茶藨子(Ribes spicatum)、高山茶藨子(Ribesbes alpinum)、Ribes schlechtendalii、多花茶藨子(Ribes multiflorum)、岩生茶藨子(Ribes petraeum)、伏生茶藨子(Ribes triste)),黑醋栗(例如,黑茶藨子(Ribes nigrum),甜瓜(例如,西瓜、冬瓜、卡萨巴甜瓜、哈密瓜、白兰瓜、香瓜、西瓜(Citrullus lanatus)、冬瓜(Benincasa hispida)、罗马甜瓜(Cucumis melocantalupensis)、冬甜瓜(Cucumis melo inodorus)、网纹甜瓜(Cucumis meloreticulatus)),黄瓜(例如,切黄瓜、腌制黄瓜、英国黄瓜、黄瓜(Cucumis sativus)),南瓜(例如,美国南瓜(Cucurbita pepo)、笋瓜(Cucurbita maxima)),倭瓜(例如,葫芦、西葫芦(Cucurbita argyrosperma)、黑子南瓜(Cucurbita ficifolia)、笋瓜(Cucurbitamaxima)、中国南瓜(Cucurbita moschata)),葡萄(例如,葡萄(Vitis vinifera)、山葡萄(Vitis amurensis)、美洲葡萄(Vitis labrusca)、白亮葡萄(Vitis mustangensis)、河岸葡萄(Vitis riparia)、圆叶葡萄(Vitis rotundifolia))或大麻(例如,印度大麻、大麻(Cannabis sativa))。 在一些方面中,本公开涉及从以上实施方式的任何一项的基因改变的植物种子产生的植物,其中植物表达异源多肽,并且其中异源多肽的表达允许植物通过异源受体多肽识别脂壳寡糖(LCO)。 在一些方面中,本公开涉及能够识别含有至少一种经修饰的核酸序列的LCO的植物或其部分,所述经修饰的核酸序列含有植物或其部分中高亲和性和/或高选择性LCO受体的至少一种编码序列,其中LCO受体在植物或其部分中表达;其中LCO受体的表达允许植物来识别LCO。在一些实施方式中,本公开涉及能够识别含有至少一种经修饰的核酸序列具有高亲和性和/或高选择性的LCO的植物或其部分,所述修饰核酸序列含有植物或其部分中高亲和性和/或高选择性LCO受体的至少一种编码序列,其中高亲和性和/或高选择性LCO受体在植物或其部分中表达;其中高亲和性和/或高选择性LCO受体的表达允许植物来识别具有高亲和性和/或高选择性的LCO。在一些实施方式中,LCO受体来自豆类。 LysM受体是熟知的和很好理解的受体类型。LysM受体具有位于蛋白质的胞外域的三个特征结构域:LysM1、LysM2和LysM3,其在蛋白质序列上以该顺序存在。LysM1结构域朝向蛋白质序列的N-末端定位,并且先于N-末端信号肽以及C(x)xxxC基序。LysM1结构域通过CxC基序与LysM2结构域分开并且LysM2结构域也通过CxC基序与LysM3结构域分开。三个LysM基序以及C(x)xxxC和CxC基序在图8A-8C中清楚地显示。图9A-9B、图10A-10B和图11A-11B显示Nod因子(例如,LCO)LysM受体、EPS LysM受体和几丁质(CO)以及PGN LysM受体的个别比对,再次清楚地描绘了三个LysM基序以及C(x)xxxC和CxC基序。因此,LysM受体的分类对本领域技术人员是已知的。 如本公开中使用的,术语“选择性”指不同多糖配体之间,具体地作为类别的脂壳寡糖(LCO)和其他多糖配体,优选地壳寡糖(CO)之间的差异。本公开的LysM受体在其LysM2结构域中含有疏水补丁。该疏水补丁赋予超过CO的LCO的选择性识别,并且因此与不具有疏水补丁的LysM受体相比,具有疏水补丁的LysM受体具有高选择性。 如本公开中使用的,术语“亲和性”通常指对LCO的亲和性。此外,本公开的LysM受体的LysM2结构域中存在的疏水补丁赋予对LCO的更高亲和性。因此,与不具有疏水补丁的LysM受体相比,具有疏水补丁的LysM受体具有高亲和性。亲和性可使用以下示例中描述的方法,以及使用本领域已知的测量结合动力学、缔合、解离和K
本公开的某些方面涉及产生任何以上实施方式的基因改变的植物的方法,其包括将基因改变引入包括核酸序列的植物。在一些实施方式中,核酸序列可操作地连接至启动子。在一些实施方式中,启动子是根特异性启动子。在一些实施方式中,启动子选自下述的组中:NFR1或NFR5/NFP启动子、莲属NFR5启动子(SEQ ID NO:24)和莲属NFR1启动子(SEQ IDNO:25)、玉蜀黍甲硫氨酸启动子、几丁质酶启动子、玉蜀黍ZRP2启动子、番茄LeExtl启动子、谷氨酰胺合酶大豆根启动子、RCC3启动子、水稻antiquitine启动子、LRR受体激酶启动子或拟南芥pCO2启动子。在一些实施方式中,启动子是任选地选自下述的组中的组成型启动子:CaMV35S启动子、CaMV35S启动子的衍生物、玉蜀黍泛素启动子、三叶启动子、静脉花叶木薯病毒启动子或拟南芥UBQ10启动子。在一些实施方式中,核酸序列被插入植物的基因组,以便核酸序列可操作地连接至内源启动子。在一些实施方式中,内源启动子是根特异性启动子。 本公开的某些方面涉及产生能够识别LCO的基因改变的植物的方法,其包括步骤:将基因改变引入植物,包括提供由固氮菌和/或菌根真菌产生的LCO得到识别的能力,从而使植物能够识别LCO。 在一些方面中,本公开涉及产生能够识别LCO的基因改变的植物的方法,其包括步骤:将基因改变引入植物,包括提供具有高亲和性和/或高选择性的由固氮菌和/或菌根真菌产生的LCO得到识别的能力,从而使植物能够识别具有高亲和性和/或高选择性的LCO。 在一些方面中,本公开涉及培养具有识别LCO的能力的植物的方法,其包括步骤:提供具有一个或多个基因改变的种子,所述基因改变提供由固氮菌和/或菌根真菌产生的LCO得到识别的的能力,其中种子产生具有识别由固氮菌和/或菌根真菌产生的LCO的能力的植物;在一定条件下培养植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物生长相比,识别由固氮菌和/或菌根真菌产生的LCO的能力导致生长、产率和/或生物质增加。在一些实施方式中,植物在营养贫瘠的土壤中栽培。 在一些方面中,本公开涉及培养具有识别具有高亲和性和/或高选择性的LCO的能力的植物的方法,其包括步骤:提供具有一个或多个基因改变的种子,所述基因改变提供具有高亲和性和/或高选择性的由固氮菌和/或菌根真菌产生的LCO得到识别的的能力,其中种子产生具有识别具有高亲和性和/或高选择性的由固氮菌和/或菌根真菌产生的LCO的能力的植物;在一定条件下培养植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,识别具有高亲和性和/或高选择性的由固氮菌和/或菌根真菌产生的LCO的能力导致生长、产率和/或生物质增加。在一些实施方式中,植物在营养贫瘠的土壤中栽培。 在一些方面中,本公开涉及培养具有识别LCO的能力的植物的方法,其包括步骤:提供具有一个或多个基因改变的组织培养物或原生质体,所述基因改变提供由固氮菌和/或菌根真菌产生的LCO得到识别的的能力;使组织培养物或原生质体再生为小植株;使小植株生长为植物,其中植物具有识别由固氮菌和/或菌根真菌产生的LCO的能力;移植植物至一定的条件,其中与在相同条件下生长的缺少一个或多个基因改变的植物生长相比,识别由固氮菌和/或菌根真菌产生的LCO的能力导致生长、产率和/或生物质增加。在一些实施方式中,植物在营养贫瘠的土壤中栽培。 在一些方面中,本公开涉及培养具有识别具有高亲和性和/或高选择性的LCO的能力的植物的方法,其包括步骤:提供具有一个或多个基因改变的组织培养物或原生质体,所述基因改变提供具有高亲和性和/或高选择性的由固氮菌和/或菌根真菌产生的LCO得到识别的的能力,使组织培养物或原生质体再生为小植株;使小植株生长为植物,其中植物具有识别具有高亲和性和/或高选择性的由固氮菌和/或菌根真菌产生的LCO的能力;移植植物至一定的条件,其中与在相同条件下生长的缺少一个或多个基因改变的植物生长相比,识别具有高亲和性和/或高选择性的由固氮菌和/或菌根真菌产生的LCO的能力导致生长、产率和/或生物质增加。在一些实施方式中,植物在营养贫瘠的土壤中栽培。 在任何以上方法的一些实施方式中,识别LCO的能力由编码异源受体多肽的核酸序列赋予,其中异源受体多肽选自下述的组中:与SEQ ID NO:5(即鹰嘴豆,鹰嘴豆(Cicerarietinum)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第一多肽,与SEQ ID NO:7(即菜豆,菜豆(Phaseolus vulgaris)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第二多肽,与SEQ ID NO:9(即花生,花生属(Arachis)NFR5)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第三多肽,与SEQ ID NO:11(即莲属(Lotus)LYS11)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第四多肽,与SEQ ID NO:12(即苜蓿属(Medicago)LYR1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第五多肽,与SEQ ID NO:13(即拟山黄麻属(Parasponia)NFP1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第六多肽,与SEQ ID NO:16(即大麦HvLysM-RLK1(AK370300))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第七多肽,与SEQ ID NO:17(即大麦HvLysM-RLK2(AK357612))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第八多肽,与SEQ ID NO:18(即大麦HvLysM-RLK3 AK372128)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第九多肽,与SEQ ID NO:19(即大麦HvLysM-RLK10(HORVU4Hr1G066170))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十多肽,与SEQ ID NO:20(即玉蜀黍ZM1(XP_020399958))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十一多肽,与SEQ ID NO:21(即玉蜀黍ZM5(XP_008652982.1))具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十二多肽,与SEQ ID NO:22(即苹果NFP5 XP_008338966.1)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十三多肽或与SEQ ID NO:23(即草莓NFR5XP_004300586.2)具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%或至少99%序列同一性的第十四多肽。在一些实施方式中,异源受体多肽选自下述的组中:SEQ ID NO:5(即鹰嘴豆,鹰嘴豆(Cicer arietinum)NFR5)、SEQ ID NO:7(即菜豆,菜豆(Phaseolus vulgaris)NFR5)、SEQ ID NO:9(即花生,花生属(Arachis)NFR5)、SEQ ID NO:11(即莲属(Lotus)LYS11)、SEQ ID NO:12(即苜蓿属(Medicago)LYR1)、SEQ ID NO:13(即拟山黄麻属(Parasponia)NFP1)、SEQ ID NO:16(即大麦HvLysM-RLK1(AK370300))、SEQ ID NO:17(即大麦HvLysM-RLK2(AK357612))、SEQ ID NO:18(即大麦HvLysM-RLK3AK372128)、SEQ ID NO:19(即大麦HvLysM-RLK10(HORVU4Hr1G066170))、SEQ ID NO:20(即玉蜀黍ZM1(XP_020399958))、SEQ ID NO:21(即玉蜀黍ZM5(XP_008652982.1))、SEQ ID NO:22(即苹果NFP5XP_008338966.1)和SEQ ID NO:23(即草莓NFR5 XP_004300586.2)。在一些实施方式中,异源受体多肽的表达允许植物或其部分通过异源受体多肽识别脂壳寡糖(LCO)。在一些实施方式中,LCO由固氮菌或由菌根真菌产生。在一些实施方式中,LCO由选自下述的组中的固氮菌产生:百脉根中慢生根瘤菌(Mesorhizobium loti)、华癸中慢生根瘤菌(Mesorhizobiumhuakuii)、地中海中慢生根瘤菌(Mesorhizobium mediterraneum)、鹰嘴豆中慢生根瘤菌(Mesorhizobium ciceri)、中慢生根瘤菌属(Mesorhizobium)物种、蒙古根瘤菌(Rhizobiummongolense)、热带根瘤菌(Rhizobium tropici)、菜豆埃特里根瘤菌(Rhizobium etliphaseoli)、贾氏根瘤菌(Rhizobium giardinii)、豌豆根瘤菌(Rhizobium leguminosarum)任选地三叶草豌豆根瘤菌(R.leguminosarum trifolii)、蚕豆豌豆根瘤菌(R.leguminosarum viciae)和菜豆豌豆根瘤菌(R.leguminosarum phaseoli)、伯克氏菌目(Burkholderiales)任选地含羞草的共生体、苜蓿中华根瘤菌(Sinorhizobium meliloti)、药用中华根瘤菌(Sinorhizobium medicae)、费氏中华根瘤菌(Sinorhizobium fredii)、费氏中华根瘤菌(Sinorhizobium fredii)NGR234、茎瘤固氮根瘤菌(Azorhizobiumcaulinodans)、大豆慢生根瘤菌(Bradyrhizobium japonicum)、埃氏慢生根瘤菌(Bradyrhizobium elkanii)、辽参慢生根瘤菌(Bradyrhizobium liaonginense)、根瘤菌属(Rhizobium)物种、中慢生根瘤菌属(Mesorhizobium)物种、中华根瘤菌属(Sinorhizobium)物种、固氮根瘤菌属(Azorhizobium)物种、弗兰克氏菌属(Frankia)物种或其任何组合,或由选自下述的组中的菌根真菌产生:无柄孢子科(Acaulosporaceae)物种、多孢囊霉科(Diversisporaceae)物种、巨孢囊霉科(Gigasporaceae)物种、平囊霉科(Pacisporaceae)物种、管柄囊霉属(Funneliformis)物种、球囊霉属(Glomus)物种、球囊霉属(Rhizophagus)物种、硬囊霉属(Sclerocystis)物种、Septoglomus属物种、Claroideoglomus属物种、Ambispora属物种、原囊霉属(Archaeospora)物种、Geosiphon pyriformis属物种、类球囊霉属(Paraglomus)属物种、球囊霉门(Glomeromycota)中的其他物种或其任何组合产生。在一些实施方式中,异源多肽位于植物细胞质膜。在一些实施方式中,植物细胞是根细胞。在一些实施方式中,根细胞是根表皮细胞或根皮质细胞。在一些实施方式中,异源多肽在发育中的植物根系中表达。在一些实施方式中,核酸序列可操作地连接至启动子。在一些实施方式中,启动子是根特异性启动子。在一些实施方式中,启动子选自下述的组中:NFR1或NFR5/NFP启动子、莲属NFR5启动子(SEQ ID NO:24)和莲属NFR1启动子(SEQ ID NO:25)、玉蜀黍甲硫氨酸启动子、几丁质酶启动子、玉蜀黍ZRP2启动子、番茄LeExtl启动子、谷氨酰胺合酶大豆根启动子、RCC3启动子、水稻antiquitine启动子、LRR受体激酶启动子或拟南芥pCO2启动子。在一些实施方式中,启动子是任选地选自下述的组中的组成型启动子:CaMV35S启动子、CaMV35S启动子的衍生物、玉蜀黍泛素启动子、三叶启动子、静脉花叶木薯病毒启动子或拟南芥UBQ10启动子。在一些实施方式中,植物选自下述的组中:玉米(例如,玉蜀黍、玉米(Zeamays)),水稻(例如,亚洲栽培稻(Oryza sativa)、非洲栽培稻(Oryza glaberrima)、菰属(Zizania)物种,大麦(例如,大麦(Hordeum vulgare),小麦(例如,普通小麦、斯卑尔脱小麦、硬粒小麦、普通小麦(Triticum aestivum)、斯卑尔脱小麦(Triticum spelta)、硬粒小麦(Triticum durum)、小麦属(Triticum)物种),山黄麻属(Trema)物种(例如,光叶山黄麻(Trema cannabina)、Trema cubense、Trema discolor、Trema domingensis、Tremaintegerrima、Trema lamarckiana、小花山黄麻(Trema micrantha)、异色山黄麻(Tremaorientalis)、Trema philippinensis、Trema strigilosa、山黄麻(Trema tomentosa)),苹果(例如,西洋苹果(Malus pumila),梨(例如,西洋梨(Pyrus communis)、白梨(Pyrus×bretschneideri)、沙梨(Pyrus pyrifolia)、新疆梨(Pyrus sinkiangensis)、川梨(Pyruspashia)、梨属(Pyrus)物种),李子(例如,西梅、西洋李子,布拉斯李、欧洲李(Prunusdomestica)、中国李(Prunus salicina)),杏子(例如,山杏(Prunus armeniaca)、布里扬松杏(Prunus brigantina)、东北杏(Prunus mandshurica)、梅(Prunus mume)、西伯利亚杏(Prunus sibirica)),桃子(例如,油桃、桃(Prunus persica),扁桃(例如,甜扁桃(Prunusdulcis)、扁桃(Prunus amygdalus)),胡桃(例如,波斯胡桃、英国胡桃、黑胡桃、胡桃(Juglans regia)、黑胡桃(Juglans nigra)、白胡桃(Juglans cinerea)、加州胡桃(Juglans californica)),草莓(例如,草莓(Fragaria×ananassa)、智利草莓(Fragariachiloensis)、弗州草莓(Fragaria virginiana)、野草莓(Fragaria vesca)),树莓(例如,欧洲红树莓、黑树莓、覆盆子(Rubus idaeus)、美国黑树莓(Rubus occidentalis)、硬毛树莓(Rubus strigosus)),黑莓(例如,常青黑莓,喜马拉雅黑莓、黑莓(Rubus fruticosus)、北美黑莓(Rubus ursinus)、裂叶黑莓(Rubus laciniatus)、尖齿黑莓(Rubus argutus)、亚美尼亚黑莓(Rubus armeniacus)、露莓(Rubus plicatus)、榆叶黑莓(Rubus ulmifolius)、美国黑莓(Rubus allegheniensis)),红醋栗(例如,红丛茶蔍子(Ribes rubrum)、红茶藨子(Ribes spicatum)、高山茶藨子(Ribesbes alpinum)、Ribes schlechtendalii、多花茶藨子(Ribes multiflorum)、岩生茶藨子(Ribes petraeum)、伏生茶藨子(Ribes triste)),黑醋栗(例如,黑茶藨子(Ribes nigrum),甜瓜(例如,西瓜、冬瓜、卡萨巴甜瓜、哈密瓜、白兰瓜、香瓜、西瓜(Citrullus lanatus)、冬瓜(Benincasa hispida)、罗马甜瓜(Cucumis melocantalupensis)、冬甜瓜(Cucumis melo inodorus)、网纹甜瓜(Cucumis meloreticulatus)),黄瓜(例如,切黄瓜、腌制黄瓜、英国黄瓜、黄瓜(Cucumis sativus)),南瓜(例如,美国南瓜(Cucurbita pepo)、笋瓜(Cucurbita maxima)),倭瓜(例如,葫芦、西葫芦(Cucurbita argyrosperma)、黑子南瓜(Cucurbita ficifolia)、笋瓜(Cucurbitamaxima)、中国南瓜(Cucurbita moschata)),葡萄(例如,葡萄(Vitis vinifera)、山葡萄(Vitis amurensis)、美洲葡萄(Vitis labrusca)、白亮葡萄(Vitis mustangensis)、河岸葡萄(Vitis riparia)、圆叶葡萄(Vitis rotundifolia))或大麻(例如,印度大麻、大麻(Cannabis sativa))。
本发明的一个实施方式提供了包括一个或多个修饰的植物基因和/或引入的基因的基因改变的植物或植物细胞。例如,本公开提供了具有编码异源受体多肽的核酸序列的基因改变的植物。异源受体允许植物通过异源受体多肽识别脂壳寡糖(LCO)。 基因改变的单子叶植物和双子叶植物植物细胞的转化和生成是本领域熟知的。参见,例如,Weising等,Ann.Rev.Genet.22:421-477(1988);美国专利5,679,558;Agrobacterium Protocols,ed:Gartland,Humana Press Inc.(1995)和Wang等ActaHort.461:401-408(1998)。方法的选择随着待转化的植物的类型、特别的应用和/或所需结果而变化。熟练的技术人员很容易选择适当的转化技术。 本领域已知的删除、插入或以其他的任何方法修饰细胞DNA(例如,基因组DNA和细胞器DNA)可用于实践本文公开的发明。作为示例,CRISPR/Cas-9和相关系统可用于将异源基因插入基因组DNA中的靶向位点或基本上编辑内源基因以表达异源基因。例如,含有用于根癌农杆菌中靶基因的删除或插入的遗传构建体的非致瘤Ti质粒可用于转化植物细胞,并且其后,转化植物可使用本领域中,例如,EP 0116718、EP 0270822、PCT公布WO 84/02913和公布的欧洲专利申请(“EP”)0242246中描述的方法从转化的植物细胞中再生。Ti-质粒载体各自含有Ti-质粒的T-DNA的边界序列之间或至少位于右侧边界序列的左侧的基因。当然,其他类型的载体可用于转化植物细胞,使用如下过程比如直接基因转移(如例如EP0233247中描述的)、花粉介导的转化(如例如EP 0270356、PCT公布WO 85/01856和US专利4,684,611中描述的)、植物RNA病毒介导的转化(如例如EP 0067553和US专利4,407,956中描述的)、脂质体介导的转化(如例如US专利4,536,475中描述的)和其他方法比如用于转化玉米(例如,US 专利6,140,553;Fromm等,Bio/Technology(1990)8,833 839);Gordon-Kamm等,The Plant Cell,(1990)2,603 618)和水稻(Shimamoto等,Nature,(1989)338,274276;Datta等,Bio/Technology,(1990)8,736 740)的某些系的方法和一般用于转化单子叶植物的方法(PCT公布WO 92/09696)。对于棉花转化,可使用PCT专利公开WO 00/71733中描述的方法。对于大豆转化,参考本领域已知的方法制造,例如,Hinchee等(Bio/Technology,(1988)6,915)和Christou等(Trends Biotech,(1990)8,145)或WO 00/42207的方法。 本发明的基因改变的植物可用于常规的植物培育方案以产生更多具有相同特征的基因改变的植物或将基因改变引入其他品种的相同或相关植物物种中。从改变的植物获得的种子优选地含有基因改变作为在染色体或细胞器DNA中的稳定插入片段或者作为对内源基因或启动子的修饰。按照本发明的包括基因改变的植物包括包含或源自包含本发明的基因改变的植物,例如果树或观赏植物的根状茎的植物。因此,本发明中包括插入转化植物或植物部分的任何非转基因嫁接植物部分。 无论是否在表达载体或表达盒中,引入的基因元件导致引入的基因的表达通常将利用植物可表达的启动子。如本文使用的“植物可表达的启动子”指确保植物细胞中本发明的基因改变的表达的启动子。指导植物中组成型表达的启动子的示例是本领域已知的并且其包括:黄瓜花叶病毒(CaMV),例如,分离株CM 1841(Gardner等,Nucleic Acids Res,(1981)9,2871 2887)、CabbB S(Franck等,Cell(1980)21,285 294)和CabbB JI(Hull和Howell,Virology,(1987)86,482 493)的强组成型35S启动子(“35S启动子”);泛素家族的启动子(例如,Christensen等,Plant Mol Biol,(1992)18,675-689的玉蜀黍泛素启动子),gos2启动子(de Pater等,The Plant J(1992)2,834-844),emu启动子(Last等,Theor ApplGenet,(1990)81,581-588),肌动蛋白启动子比如通过An等(The Plant J,(1996)10,107)描述的启动子,通过Zhang等(The Plant Cell,(1991)3,1155-1165)描述的水稻肌动蛋白启动子;木薯静脉花叶病毒的启动子(WO 97/48819,Verdaguer等(Plant Mol Biol,(1998)37,1055-1067),来自地下三叶草矮化病毒的启动子的pPLEX系列(WO 96/06932,特别地S4或S7启动子),醇脱氢酶启动子,例如,pAdh1S(GenBank accession numbers X04049,X00581)以及分别驱动T DNA的1'和2'基因的表达的TR1'启动子和TR2'启动子(分别为“TR1'启动子”和“TR2'启动子”)(Velten等,EMBO J,(1984)3,2723 2730)。 可选地,植物可表达的启动子可以是组织特异性启动子,例如,指导植物的一些细胞或组织中,例如,根表皮细胞中的更高水平的表达启动子。通常在植物细胞中使用的组成型启动子的示例是花椰菜花叶(CaMV)35S启动子(KAY等Science,236,4805,1987)和该启动子的各种衍生物,玉蜀黍泛素启动子(CHRISTENSEN&QUAIL,Transgenic Res,5,213-8,1996),三叶启动子(Ljubql,MAEKAWA等Mol Plant Microbe Interact.21,375-82,2008),静脉花叶木薯病毒启动子(国际申请WO 97/48819)和拟南芥UBQ10启动子(Norris等,PlantMol.Biol.21,895-906,1993)。 在优选的实施方式中,将使用根特异性启动子。非限制性示例包括NFR1或NFR5/NFP启动子,特别地莲属NFR5启动子(SEQ ID NO:24)和莲属NFR1启动子(SEQ ID NO:25)、玉蜀黍甲硫氨酸启动子(DE FRAMOND等,FEBS 290,103-106,1991申请EP 452269)、几丁质酶启动子(SAMAC等,Plant Physiol 93,907-914,1990)、玉蜀黍ZRP2启动子(美国专利号5,633,363)、番茄LeExtl启动子(Bucher等,Plant Physiol.128,911-923,2002)、谷氨酰胺合酶大豆根启动子(HIREL等,Plant Mol.Biol.20,207-218,1992)、RCC3启动子(PCT申请WO2009/016104)、水稻antiquitine启动子(PCT申请WO 2007/076115)、LRR受体激酶启动子(PCT申请WO 02/46439)和拟南芥pCO2启动子(HEIDSTRA等,Genes Dev.18,1964-1969,2004)。这些植物启动子可与增强子元件组合,它们可与最小的启动子元件组合,或可包括重复元件以确保所需表达特征。 在一些实施方式中,可利用在植物细胞中增加表达的基因元件。例如,引入的基因的5’端或3’端处或者在编码引入的基因的序列中的内含子,例如,hsp70内含子。其他这种基因元件可包括但不限于启动子增强子元件、两次重复或三次重复的启动子区域、与另一转基因不同或与内源(植物宿主)基因前导序列不同的5’前导序列、与相同植物中使用的另一转基因不同或与内源(植物宿主)尾随序列不同的3’尾随序列。 本发明的引入的基因可被插入宿主细胞DNA,以便插入的基因部分是合适的3'端转录调节信号(例如,转录体形成和多腺苷酸化信号)的上游(即5')。这优选地通过将基因插入植物细胞基因组(细胞核或叶绿体)中完成。优选的多腺苷酸化和转录体形成信号包括胭脂氨酸合酶基因(Depicker等,J.Molec Appl Gen,(1982)1,561-573)、章鱼碱合酶基因(Gielen等,EMBO J,(1984)3:835 845)、SCSV或苹果酸酶末端(Schunmann等,Plant FunctBiol,(2003)30:453-460)和T DNA基因7(Velten和Schell,Nucleic Acids Res,(1985)13,6981 6998)的那些,其在转化的植物细胞中充当3'非翻译DNA序列。在一些实施方式中,将一个或多个引入的基因稳定地整合入细胞核基因组。当核酸序列保持整合入细胞核基因组且通过随后植物世代继续被表达(例如,产生可检测的mRNA转录体或蛋白质),存在稳定的整合。可通过任何本领域已知的方法(例如,微粒轰击、农杆菌属介导的转化、CRISPR/Cas9、原生质体的电穿孔、微注射等)完成稳定整合入细胞核基因组和/或编辑细胞核基因组。 术语重组体或修饰的核酸指通过两个其他分开的序列区段的组合制造的多核苷酸,这是通过基因工程化技术或通过化学合成的由人工操作分离的多核苷酸的区段完成的。这样做可将所需功能的多核苷酸片段连接以产生所需功能的组合。 如本文使用的,术语“过度表达”和“上调”指作为基因修饰的结果相对于野生型生物体(例如,植物)中的表达增加的表达(例如,mRNA、多肽等)。在一些实施方式中,表达的增加比野生型中的表达略微增加约10%。在一些实施方式中,表达的增加相对于野生型中的表达增加50%或更多(例如,60%、70%、80%、100%等)。在一些实施方式中,内源基因是过度表达的。在一些实施方式中,外源基因由于被表达而过度表达。植物中基因的过度表达可通过任何本领域已知的方法实现,其包括但不限于成型启动子、诱导型启动子、高表达启动子(例如,PsaD启动子)、增强子、转录和/或翻译调节序列、密码子优化、修饰的转录因子和/或控制待过度表达的基因的表达的经突变或经修饰基因的使用。 在重组核酸旨在用于特定序列的表达、克隆或复制时,用于引入宿主细胞而制备的DNA构建体通常将包括由宿主识别的复制系统(例如载体),包括编码所需多肽的预期的DNA片段,并且还可包括可操作地连接至编码多肽的区段的转录和翻译启动调节序列。可选地,这种构建体可包括细胞定位信号(例如,质膜定位信号)。在优选的实施方式中,这种DNA构建体被引入宿主细胞的基因组DNA、叶绿体DNA或线粒体DNA。 在一些实施方式中,非整合的表达系统可用于诱导一个或多个引入的基因的表达。表达系统(表达载体)可包括,例如,复制或自主复制序列(ARS)的起点和表达控制序列、启动子、增强子和必要的加工信息位点,比如核糖体-结合位点、RNA剪接位点、多腺苷酸化位点、转录终止子序列和mRNA稳定化序列。也可适当的包括来自相同或相关物种的分泌多肽的信号肽,其允许蛋白质跨过和/或嵌入细胞膜、细胞壁,或从细胞中分泌。 用于实践本文公开的发明的方法的可选择标志物可以是正向可选择标志物。典型地,正向选择指在仅在细胞内存在目标重组体多核苷酸时,基因改变的细胞可在有毒的物质存在下存活的情况。负向可选择标志物和可筛选标志物在本领域也是熟知的且是本发明考虑的。本领域技术人员将认识到,任何可获得的相关标志物可在实践本文公开的发明中利用。 本发明的重组菌的筛选和分子分析可利用核酸杂交技术进行。杂交方法用于鉴定多核苷酸,比如使用本文描述的技术修饰的那些,其与本文教导使用的主题调节序列具有足够的同源性。特别的杂交技术对本发明不是必需的。随着杂交技术中进行的改善,它们可由本领域技术人员容易地应用。杂交探针可用本领域技术人员已知的任何适当的标记进行标记。可改变杂交条件和冲洗条件,例如温度和盐浓度,以改变检测阈值的严谨性。参见,例如,Sambrook等(1989)参见下文或Ausubel等(1995)Current Protocols in MolecularBiology,John Wiley&Sons,NY,N.Y.,用于杂交条件的进一步指导。 可选地,基因改变的株系的筛选和分子分析以及所需分离的核酸的产生可使用聚合酶链式反应(PCR)进行。PCR是核酸序列的重复、酶促、引发合成。该方法对该领域的那些技术人员是熟知的和常用的(参见Mullis,美国专利号4,683,195、4,683,202和4,800,159;Saiki等(1985)Science230:1350-1354)。PCR基于目标DNA片段的酶促扩增,目标DNA片段的侧面是与靶序列的相对链杂交的两个寡核苷酸引物。引物以指向彼此的3’端定向。模板的热变性、引物退火至它们互补的序列以及用DNA聚合酶延伸退火的引物的重复循环导致对由PCR引物的5’端限定的区段的扩增。因为每个引物的延伸产物可用作其他引物的模板,每个循环实质上使前一个循环中产生的DNA模板的量加倍。这导致特定靶片段的指数积聚,在几小时内达到几百万倍。通过使用热稳定DNA聚合酶比如Taq聚合酶,其分离自嗜热性细菌水生栖热菌(Thermus aquaticus),可完全自动化扩增过程。可使用的其他酶对本领域技术人员是已知的。 本发明的核酸和蛋白质也可涵盖具体地公开序列的同系物。同源性(例如,序列同一性)可以是50%-100%。在一些情况下,这种同源性大于80%、大于85%、大于90%或大于95%。序列的任何预期用途需要的同源性或同一性的程度容易由本领域技术人员鉴定。如本文使用的两个核酸的序列同一性百分比使用本领域已知的算法,比如由Karlin和Altschul(1990)Proc.Natl.Acad.Sci.USA 87:2264-2268公开的,Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877中修饰的算法确定。这种算法被并入Altschul等(1990)J.Mol.Biol.215:402-410的NBLAST和XBLAST程序中。BLAST核苷酸搜索用NBLAST程序进行,评分=100,字长=12,以获得具有所需序列同一性百分比的核苷酸序列。为了获得用于比较的目的的间隙比对,如Altschul等(1997)Nucl.Acids.Res.25:3389-3402中描述的使用间隙BLAST。当利用BLAST和间隙BLAST程序时,使用各自方法(NBLAST和XBLAST)的缺省参数。参见www.ncbi.nih.gov。 优选的宿主细胞是植物细胞。在本上下文中,重组体宿主细胞是那些已经基因修饰以含有分离的核酸分子,含有在宿主细胞中通常存在和有功能的一个或多个删除的或其他非功能基因,或含有产生至少一种重组体蛋白质的一个或多个基因。本发明的编码蛋白质的核酸可通过任何本领域已知的对特定类型的细胞适当的方法引入,所述方法包括但不限于转化、脂质转染、电穿孔或本领域技术人员已知的任何其他方法。 已经一般地描述了本发明,本发明将通过参考某些具体的实施例更好的理解,所述具体的实施例包括在本文中以进一步示例说明本发明并且不旨在限制由权利要求限定的本发明的范围。 实施例 在以下实施例中进一步详细描述本公,这些实施例不旨在以任何方式限制所要求保护的本公开的范围。附图意在被认为是本公开的说明书和描述的组成部分。提供以下实施例以举例说明,但不限制要求保护的公开内容。 实施例1:苜蓿属NFP胞外域的表达和纯化 以下实施例描述了用于制备所有以下实施例的蛋白质的蛋白质表达和纯化材料和方法。 材料和方法 苜蓿属NFP胞外域的表达和纯化:蒺藜苜蓿NFP胞外域(残基28-246)经密码子优化用于昆虫细胞表达(Genscript,Piscataway,USA)并且克隆至pOET4杆状病毒转移载体(Oxford Expression Technologies)中。天然NFP信号肽(残基1-27,通过SignalP 4.1预测的)用AcMNPV gp67信号肽代替以促进分泌并且将六组氨酸标签添加至C-末端。使用定点诱变工程化NFP的点突变体。用Lipofectin(ThermoFisher Scientific)作为转染试剂根据制造商的指导,使用FlashBac Gold试剂盒(Oxford Expression technologies)在Sf9细胞(草地夜蛾(Spodoptera frugiperda))中产生重组杆状病毒。蛋白质表达如下进行。在补充有1%Pen-Strep(10000U/ml,Life technologies)和1%CD脂质浓缩物(Gibco)的无血清MAX-XP(BD-Biosciences,停产)或HyClone SFX(GE Healthcare)培养基中,以299K摇动维持悬浮培养的Sf9细胞。一旦Sf9细胞达到1.0*10^6个细胞/ml的细胞密度,通过添加重组体3代病毒诱导蛋白质表达。在表达的5-7天后,通过离心收获含有NFP胞外域的培养基上清液。随后是在277K,针对50mM Tris-HCl pH 8,200mM NaCl的过夜透析步骤。通过Ni-IMAC纯化的两个随后步骤(HisTrap excel/HisTrap HP,都是GE Healthcare)来富集NFP胞外域。对于晶体学实验,使用内切糖苷酶PNGase F(1:15(w/w),室温,过夜)去除N-聚糖。作为最后的纯化步骤,在补充至总计500mM NaCl(对于结合测定)或50mM Tris-HCl,200mM NaCl(对于晶体学),在pH 7.2的磷酸盐缓冲盐水中,在Superdex 200 10/300或HiLoadSuperdex200 16/600(都是GE Healthcare)上通过SEC纯化NFP胞外域。NFP胞外域作为对应于单体的单一均质峰洗脱。NFP点突变的版本根据相同方案进行表达和纯化。 实施例2:苜蓿属NFP胞外域的结构表征 以下实施例描述了苜蓿属NFP蛋白质胞外域的结构表征。 材料和方法 结晶和结构测定:在0.2M醋酸钠、0.1M二甲胂酸钠pH 6.5和30%(w/v)PEG-8000中,以3-5mg/ml使用蒸汽扩散设定获得去糖基化的NFP胞外域的晶体(参见实施例1)。在液氮中速冻之前,通过补充5%(w/v)PEG-400在其结晶条件中对晶体进行冷冻保护。在MaxLabI911-3光束线处获得至 小角度X-射线散射(SAXS):在磷酸缓冲盐水,pH 7.4,500mM NaCl中,在EMBLP12光束线PETRA III,在20℃控制温度的隔室中,在 结果 使用基于AtCERK1的内部低B因子支架的同源性模型通过分子替换确定苜蓿属NFP-ECD的结构。以这种方式构建NFP-ECD的完整结构(残基33-233),包括在 为了确认确定的结构,在NFP-ECD上进行小角度X-射线散射(SAXS)测量。图1B显示了SAXS重建的包膜,其与确定的结构具有相同整体尺寸,并且另外良好适合于晶体结构。另外,在SAXS重建的包膜中看到了很可能源自受体的C-末端茎区域的细长脊状结构。在LCOLysM受体同系物当中,该茎区域在长度上和一定程度地在序列上是保守的,提示这些受体中的胞外域与膜需要某些距离,用于正确功能。 还进行SAXS测量以比较去糖基化的和糖基化的NFP-ECD。图14A显示了去糖基化的NFP-ECD的SAXS分析,而图14B显示了糖基化的NFP-ECD的SAXS分析。SAXS数据和重建的从头开始模型与晶体结构一致,然而另外存在细长茎样结构(图1B)。该茎区域很可能由NFP的C-末端部分组成,NFP的C-末端部分在晶体结构不可见,并且可能用于从质膜的正确距离处的NFP的胞外域的位置。图14C比较去糖基化的和糖基化的NFP-ECD的无量纲的Kratky图,并且该比较显示了聚糖对NFP-ECD的球状性具有影响。糖基化的NFP-ECD具有更球形的形状,其中峰值接近Guinier-Kratky点(虚线,图14C的左图)且更接近理想的球的表面/体积比(0.82,虚线,图14C的右图)。该更球状的形状可能由于15-20kDa的聚糖的存在。在聚糖去除后,球状性和细长形状的损失变得更明显和可见(图14C的两个图中的去糖基化的NFP-ECD)。 实施例3:不同壳寡糖(CO)、脂壳寡糖(LCO)和碳水化合物配体的NFP结合能力和亲和性 以下实施例描述了测量NFP结合能力和亲和性的实验。设计这些实验以研究NFP是否对不同配体具有不同结合能力和亲和性。 材料和方法 微量热泳动(MST):荧光标记了NFP(Protein Labeling Kit Blue NHS,NanoTemper Technologies)。恒定浓度的NFP用于测量与系列稀释的配体的结合。使用的配体是CO4几丁质低聚物(对应于苜蓿中华根瘤菌LCO-IV的主链)、CO5几丁质低聚物(对应于苜蓿中华根瘤菌LCO-V的主链)、麦芽六糖(来自苜蓿中华根瘤菌的碳水化合物)和辛糖胞外多糖(EPS)。使用S形的剂量响应模型在GraphPad Prism7软件(GraphPad Software,Inc.)中进行数据分析以获得平衡解离常数值。 生物层干涉法(BLI):在Octet RED 96系统(Pall ForteBio)上测量NFP和突变版本的NFP与配体的结合。使用的配体是LCO-IV(来自苜蓿中华根瘤菌)、LCO-V(来自苜蓿中华根瘤菌)、LCO-V(来自百脉根中慢生根瘤菌)和CO6(来自百脉根中慢生根瘤菌)。苜蓿中华根瘤菌LCO由四聚体/五聚体N-乙酰葡糖胺主链组成,所述四聚体/五聚体N-乙酰葡糖胺主链在还原末端残基上是O-硫酸化的,在非还原末端残基上是O-乙酰化的和由不饱和C16酰基进行单-N-酰化。百脉根中慢生根瘤菌LCO是五聚体N-乙酰葡糖胺,具有在非还原末端残基处的顺式-异油酸和氨基甲酰基和在还原末端残基处的2,4-O-乙酰基岩藻糖。将生物素化的配体缀合物以125-250nM的浓度固定在链霉抗生物素生物传感器(kinetic quality,Pall ForteBio)上5分钟。使用苜蓿中华根瘤菌配体(LCO-IV和LCO-V)的结合测定重复七次,而使用百脉根中慢生根瘤菌配体(LCO-V和CO6)的结合测定重复六次。在GraphPadPrism 6(GraphPad Software,Inc.)中进行数据分析。通过对针对蛋白质浓度绘制的平衡处的响应应用非线性回归(一个位点,特异性结合)确定源自稳态的平衡解离常数。通过对减去数据的非线性回归(在解离之后缔合)确定动力学参数。 结果 因为LCO由N-乙酰葡糖胺(几丁质)主链组成,第一测试测量NFP是否对分别对应于苜蓿中华根瘤菌LCO-IV和LCO-V主链的CO4和CO5几丁质低聚物具有亲和性。在微量热泳动(MST)实验中,发现NFP分别以150μM和93μM的解离常数(Kd)结合CO4和CO5。然而,NFP不能结合从苜蓿中华根瘤菌纯化的不相关碳水化合物配体麦芽六糖或寡糖胞外多糖。这显示NFP选择性结合几丁质的配体。 由于疏水粘性性质和胶束相,LCO配体难以在溶液中处理,其阻碍了精确的浓度测定。为了克服该问题,使用了以前开发的化学方法来用生物素化的接头来位点特异性标记LCO的还原端。使用生物层干涉法(BLI),发现NFP以26±0.2μM的Kd结合固定的苜蓿中华根瘤菌LCO-IV和以32±0.2μM的Kd结合苜蓿中华根瘤菌LCO-V(图2A)。有趣地,没有检测到NFP结合百脉根中慢生根瘤菌LCO-V,并且也没有检测到NFP结合百脉根中慢生根瘤菌CO6(图2B)。 进一步BLI测定显示NFP-ECD以22.3±0.1μM的平均K 这些结果显示NFP具有不同结合能力和亲和性,取决于配体。而且,这些这些结果显示NFP在由不同共生体物种产生的相同配体之间可不同(比较来自苜蓿中华根瘤菌LCO-V和百脉根中慢生根瘤菌LCO-V的结果)。这指示NFP可基于直接LCO结合来区分共生体。 实施例4:突变的脂壳寡糖(LCO)配体的NFP结合能力和亲和性 使用细菌突变体并测量LCO应用后根毛中钙瞬变的研究已经显示,末端N-乙酰葡糖胺残基上的侧链修饰在功能上很重要(Oldroyd GE1,Murray JD,Poole PS,and DownieJA.Annu Rev Genet.2011;45:119-44)。以下实施例描述了为了理解LCO侧链装饰如何使用来自苜蓿中华根瘤菌的NFP和其同源LCO配体而有助于这种显见的选择性进行的实验。 材料和方法 突变的脂壳寡糖(LCO)配体:苜蓿中华根瘤菌LCO由四聚体/五聚体N-乙酰葡糖胺主链组成,四聚体/五聚体N-乙酰葡糖胺主链在还原末端残基是O-硫酸化的,在非还原末端残基上是O-乙酰化的和由不饱和C16酰基进行单-N-酰化。本文使用的LCO配体是从苜蓿中华根瘤菌nodH、nodL、nodFE和nodFL突变体纯化的。这些突变体的每一个在LCO的末端部分上缺少一个或多个侧链修饰。图3A描绘了苜蓿中华根瘤菌LCO-IV,并且指示在每种突变体中改变侧链修饰。 配体结合测试:如在实施例3中的进行配体结合测试。 结果 图3B显示了使用突变的LCO的配体结合测试的结果。在用具有缺失硫酸修饰的nodH-LCO的测试中和在用缺少O-乙酰基基团的nodL-LCO的测试中看到结合至NFP的明显减少。这与用这些苜蓿中华根瘤菌突变体接种植物后观察到干扰结瘤和感染以及在应用这些突变的LCO之后发现减少钙瞬变一致。类似地,使用含有异油酸C18:1代替C16:2脂肪酸的nodFE-LCO的测试减少NFP结合。进一步,缺少O-乙酰基基团和含有异油酸C18:1的nodFL双突变体显示不结合NFP。这反映了在用各个苜蓿中华根瘤菌突变体的植物接种后观察到的干扰结瘤、不能诱导结节发育和缺少感染线形成(Ardourel M,等,Plant Cell.1994Oct;6(10):1357-74.)。合起来看,该数据显示,NFP受体可识别其同源LCO配体的各个修饰。 实施例5:用于脂壳寡糖(LCO)感知的重要残基的鉴定 以下实施例描述了使用结构指导方法以鉴定用于LCO感知的NFP中的重要残基。在鉴定重要残基后,产生NFP点突变且使用以上描述的配体-结合测定进行测试(参见实施例3和实施例4)。 材料和方法 结构指导残基鉴定:NFP胞外域在结构上与配体-结合CERK1对齐。然后,将静电表面电势映射到NFP胞外域的先前-开发的结构。在图4A中描绘了预测的配体-结合位置和静电表面电势。 NFP点突变的产生:用天冬氨酸残基代替NFP亮氨酸残基L147和L154。天冬氨酸与亮氨酸尺寸是类似的,但带负电,而亮氨酸是疏水的。使用位点定向诱变工程化NFP的点突变体。特别地,双突变的NFP是工程化的,其中亮氨酸残基L147和L154用天冬氨酸残基代替以产生突变体NFP L147D L154D。如实施例中描述的表达和纯化NFP的点突变版本。 NFP突变体结合测定:使用NFP野生型(WT)蛋白质的结合测定重复七次,而使用NFP突变体NFP L147D L154D的结合测定重复四次。 生物层干涉法(BLI):在Octet RED 96系统(Pall ForteBio)上测量NFP WT和NFPL147D/L154D突变体与苜蓿中华根瘤LCO-IV的结合。苜蓿中华根瘤LCO由四聚体/五聚体N-乙酰葡糖胺主链组成,所述四聚体/五聚体N-乙酰葡糖胺主链在还原末端残基上是O-硫酸化的,在非还原末端残基上是O-乙酰化的和由不饱和C16酰基进行单-N-酰化。将生物素化的配体缀合物以125-250nM的浓度固定在链霉抗生物素生物传感器(kinetic quality,Pall ForteBio)上5分钟。用于NFP WT的结合测定重复7次,且用于NFP L147D/L154D突变体的结合测定重复4次。在GraphPad Prism 6(GraphPad Software,Inc.)中进行数据分析。通过对在针对蛋白质浓度绘制的平衡处的响应应用非线性回归(一个位点,特异性结合)来确定源自稳态的平衡解离常数。通过对减去数据的非线性回归(在解离之后缔合)来确定动力学参数。结果在图16A-16C中显示。以相同方式测量拟南芥CERK1(AtCERK1)与壳五糖(CO5)和壳八糖(CO8)的结合。结果在图17A-17B中显示。 结果 图4A显示了对结合至具有预测的几丁质和LCO脂肪酸链位置的配体的NFP胞外域的建模。NFP胞外域与配体结合的CERK1的结构比对将几丁质定位在NFP的LysM2结合槽中,没有任何明显的碰撞。显著地,静电表面电势揭示了位于对接的几丁质分子的非还原部分附近的NFP胞外域上的疏水补丁,其潜在地可容纳LCO配体的脂肪酸链。两个亮氨酸残基(L147和L154)被鉴定为供给该补丁以疏水特征的残基。 为了测试这些两个残基对LCO结合的贡献,将两个残基用大小相似但带负电的天冬氨酸残基替代以产生NFP L147D L154D。有趣地是,双突变的NFP L147D L154D胞外域以近似两倍更低的亲和力结合苜蓿中华根瘤菌LCO-IV;48.0±1.0μM的Kd(图4B)。仔细检查结合动力学揭示了,在双突变体中,缔合(K LCO结合至疏水补丁突变体的生物化学分析揭示了与WT NFP-ECD相比,纯化的L147D/L154D NFP-ECD以13倍更低的亲和力(166.7±4.2μM的Kd)结合苜蓿中华根瘤菌LCO-IV(图16A-16C)。与WT NFP-ECD相比,双突变体中缔合速率(k 测量AtCERK1结合至几丁质片段的结合动力学作为比较。如在图17A-17B中显示,看到快速缔合和解离速率。这些动力学是突变体L147D/L154D NFP-ECD观察到的动力学的回归(图16B)。AtCERK1对几丁质片段的结合动力学与NFP对LCO的结合动力学明显地不同(图16A)。 同时,数据提供了证据:NFP中的疏水补丁(图16D中显示)是对LCO感知和共生信号传导至关重要的保守结构特征。 实施例6:苜蓿属nfp突变体中的互补测试 为了确认在先前的实施例中描述的生物化学观察,接下来使用多毛根转化在苜蓿属nfp突变体中进行互补测试。 材料和方法 互补测定:如在Bozsoki等(2017)(Bozsoki Z,Cheng J,Feng F,Gysel K,VintherM,Andersen KR,Oldroyd G,Blaise M,Radutoiu S,Stougaard J(2017)Receptor-mediated chitin perception in legume roots is functionally separable from Nodfactor perception.Proc Natl Acad Sci 114:E8118–E8127)中描述一般地进行构建体组装、植物生长条件、多毛根转化、结瘤和ROS试验。图5中提供了构建体的一般示意图。测试的转基因是实施例5中描述的突变的LysM受体。另外,LysM2(Q119F、K141E和T150H)中或LysM3(T216F)中测试NFP置换变体替换疏水补丁外部的残基。 结果 图6A-6B显示了互补测试的结果。图6A中显示的结果是互补测试,其中用苜蓿中华根瘤菌菌株1021接种植物。当用野生型Nfp基因转化苜蓿属nfp突变体时,看见互补,其定义为在用苜蓿中华根瘤菌菌株1021接种后49天平均每个植物5个结节。相反地,在用苜蓿中华根瘤菌菌株1021接种后,用含有双突变的NFP L147D L154D(表面残基,五个NFP其疏水特征在LysM2中)的构建体转化的根每个植物不发育任何结节。使用在LysM2(Q119F、K141E和T150H)中或LysM3(T216F)中NFP置换变体替换疏水补丁外部的残基的相应实验不影响结瘤。 使用药用中华根瘤菌接种重复这些互补实验,其已经报告以更高效率结瘤苜蓿属。图6B中显示的结果是互补测试,其中用药用中华根瘤菌接种植物。药用中华根瘤菌结果确认含有双突变的NFP L147D L154D的构建体互补不良。合起来看,这些结果显示NFP中的疏水补丁对于LCO识别和功能共生信号传导是需要的。 实施例7:疏水补丁的保护 以前,已经显示豌豆中的SYM10、莲属中的NFR5和大豆中的NFR5A对LCO感知是重要的(参见,例如,Plant Cell Physiol.2010Feb;51(2):201-14和Madsen,EB等,Nature.2003Oct 9;425(6958):637-40.)。所以,同源性建模用于确定邻近于苜蓿属NFP中鉴定的LysM2的疏水补丁是否是这些蛋白质间的保守特征。另外,进行莲属LYS11的同源性建模,并且获得莲属LYS11的晶体结构以验证同源性建模结果。 材料和方法 建模:用SWISS-MODEL进行同源性建模(Biasini 2014)。苜蓿属NFP的晶体结构作用模板模型,在其上绘制了靶受体的氨基酸序列。生成了输出pdb填充的结构模型并且使用PDB2PQR和APBS webservers(PMID:21425296)计算其静电表面电势。使用APBS工具2.1在PyMol中显示结果(DeLano,W.L.(2002).PyMOL.DeLano Scientific,San Carlos,CA,700.)。 晶体结构:在0.1M钠丙二酸盐pH 6.0和12%PEG3350中,以6.8mg/mL,使用蒸汽扩散设定获得LYS11的晶体。获得完整衍射数据并且基于AtCERK1胞外域结构(PDB坐标为4EBZ)的同源性模型作为搜索模型,使用来自PHENIX套件的Phaser通过分子替换解决相问题。分别使用COOT和PHENIX套件进行模型构建和完善。生成了输出pdb填充的结构模型和使用the PDB2PQR和APBS webservers(PMID:21425296)计算其静电表面电势。使用APBS工具2.1在PyMol中显示结果(DeLano,W.L.(2002).PyMOL.DeLano Scientific,San Carlos,CA,700.)。 结果 图7A显示了豌豆中的SYM10、莲属中的NFR5和大豆中的NFR5α的同源性建模结果。同源性建模揭示了疏水补丁确实存在于这些受体的LysM2结构域的正下方的等同位置。 为了调查该方法的预测能力,还对源自鹰嘴豆、菜豆和花生的基因组的最接近的受体同源物进行建模(图7B)。以前没有功能表征任何这些受体同源物。发现LysM2中的疏水补丁在这里也保守,预测这些蛋白质是NFP/NFR5类型的LCO受体。 进一步,在假激酶的苜蓿属NFP和莲属NFR5家族中测试了建模的诊断能力。图7C-7D显示苜蓿属LYR1和莲属LYS11二者含有指示LCO受体功能的疏水补丁,鉴于它们在AM共生中假定作用,其是感兴趣的(参见,例如,Rasmussen,SR等,Sci Rep.2016Jul 20;6:29733and Gomez,SK等,BMC Plant Biol.2009Jan 22;9:10.)。图7E显示了三个LCO受体:莲属NFR5、豌豆SYM10和大豆NFR5A的预测模型。模型显示为表面且按照它们静电表面电势着色。用黑虚线标出预测的疏水补丁并且在LysM2配体结合槽中显示CO4几丁质分子。图7F显示了比较缺乏疏水补丁的两个受体,苜蓿属LYR3和莲属LYS12。 为了在实验上验证该预测,与NFP相比,莲属LYS11具有疏水补丁,确定了莲属LYS11的晶体结构。图7G显示了比较莲属LYS11模型(左;也在图7C中)与莲属LYS11的晶体结构(右)。从晶体结构的静电表面电势,明显的是LYS11的确含有LysM2中的疏水补丁,类似于NFP的LysM2中的疏水补丁。在通过结晶学确定的LYS11实际结构中,已经通过建模预测的疏水结构域的存在证实了基于NFP的建模用于鉴定以前未表征的LCO受体同系物中的疏水补丁的能力。 合起来看,这些结果显示疏水补丁是在NFP/NFR5受体(例如,LCO受体)上发现的保守的结构指纹。因此,疏水补丁可用于预测性鉴定其他豆科植物中NFP/NFR5受体的类别,这在以前是不可能的。 实施例8:NFP/NFR5类别的非豆类LCO受体的建模 接下来,同源性建模用于确定邻近于苜蓿属NFP中鉴定的LysM2的疏水补丁是否存在于大麦LysM受体激酶。 材料和方法 建模:用SWISS-MODEL进行同源性建模(Biasini,M.等,SWISS-MODEL:modellingprotein tertiary and quaternary structure using evolutionaryinformation.Nucleic Acids Res.42,W252–W258(2014))。苜蓿属NFP的晶体结构作用模板模型,在其上绘制了靶受体的氨基酸序列。生成了输出pdb填充的结构模型并且使用PDB2PQR和APBS webservers(PMID:21425296)计算其静电表面电势。使用APBS工具2.1在PyMol中显示结果(DeLano,W.L.(2002).PyMOL.DeLano Scientific,San Carlos,CA,700.)。 大麦RLK10胞外域的表达和纯化:大麦RLK10(HvRLK10;HvLysM-RLK10)胞外域(残基25-231;SEQ ID NO:29)经密码子优化用于昆虫细胞表达(Genscript,Piscataway,USA)且克隆至pOET4杆状病毒转移载体(Oxford Expression Technologies)中。天然RLK10信号肽用gp64信号肽代替以促进分泌并且将六组氨酸(6xHIS)标签添加至C-末端,制作序列HvRLK10-ecto(25-231)、N-端gp64、C-端6His(SEQ ID NO:28)。用Lepofectin(ThermoFisher Scientific)作为转染试剂根据制造商的指导,使用FlashBac Gold试剂盒(Oxford Expression technologies)在Sf9细胞(草地夜蛾)中产生重组杆状病毒。蛋白质表达如下进行。在补充有1%Pen-Strep(10000U/ml,Life technologies)和1%CD脂质浓缩物(Gibco)的无血清的MAX-XP(BD-Biosciences,停产)或HyClone SFX(GE Healthcare)培养基中,以299K摇动维持悬浮培养的Sf9细胞。一旦Sf9细胞达到1.0*10^6个细胞/ml的细胞密度,通过添加重组体3代病毒诱导蛋白质表达。在表达的5-7天后,通过离心收获含有RLK10胞外域的培养基上清液。随后是在277K,针对50mM Tris-HCl pH 8,200mM NaCl的过夜透析步骤。通过Ni-IMAC纯化的两个后续步骤富集RLK10胞外域(HisTrap excel/HisTrapHP,都是GE Healthcare)。 配体结合测试:如实施例3中使用BLI进行配体结合测试。 结果 使用苜蓿属NFP结构作为模板进行了所有十种大麦LysM受体样激酶(RLK)的同源性建模。在大麦LysM RLK中,HvRLK10是最接近苜蓿属NFP的受体,并且使用该方法建模最佳。图13B显示了HvRLK10的同源性建模结构,其揭示了疏水补丁确实存在于该受体的LysM2结构域的正下方的等同位置。该清楚的疏水补丁指示HvRLK10是NFP/NFR5类型的LCO受体。 为了在实验上验证该预测,表达且纯化HvRLK10胞外域用于结合实验(图13A的顶部处显示胞外域示意图)。显示HvRLK10胞外域结合百脉根中慢生根瘤菌LCO(图13C)和苜蓿中华根瘤菌LCO(图13D)二者。相反地,HvRLK10胞外域不结合CO5(图13A)。这些结合研究显示预测的HvRLK10具有结合LCO,但不结合CO的疏水补丁。 合起来看,这些结果显示疏水补丁是在NFP/NFR5受体(例如,LCO受体)间发现的保守的结构指纹。该保守性从豆科植物家族延伸至非豆类植物,比如大麦。因此,疏水补丁可用于预测性鉴定非豆类植物中NFP/NFR5受体的类别,这在以前是不可能的。 序列表 奥尔胡斯大学 表达识别脂壳寡糖的异源受体的基因改变的植物 79454-20003.40 Not Yet Assigned Concurrently Herewith US 62/718,186 2018-08-13 59 Windows版本4.0的FastSEQ 1 595 PRT 蒺藜苜蓿 1 Met Ser Ala Phe Phe Leu Pro Ser Ser Ser His Ala Leu Phe Leu Val 1 5 10 15 Leu Met Leu Phe Phe Leu Thr Asn Ile Ser Ala Gln Pro Leu Tyr Ile 20 25 30 Ser Glu Thr Asn Phe Thr Cys Pro Val Asp Ser Pro Pro Ser Cys Glu 35 40 45 Thr Tyr Val Ala Tyr Arg Ala Gln Ser Pro Asn Phe Leu Ser Leu Ser 50 55 60 Asn Ile Ser Asp Ile Phe Asn Leu Ser Pro Leu Arg Ile Ala Lys Ala 65 70 75 80 Ser Asn Ile Glu Ala Glu Asp Lys Lys Leu Ile Pro Asp Gln Leu Leu 85 90 95 Leu Val Pro Val Thr Cys Gly Cys Thr Lys Asn His Ser Phe Ala Asn 100 105 110 Ile Thr Tyr Ser Ile Lys Gln Gly Asp Asn Phe Phe Ile Leu Ser Ile 115 120 125 Thr Ser Tyr Gln Asn Leu Thr Asn Tyr Leu Glu Phe Lys Asn Phe Asn 130 135 140 Pro Asn Leu Ser Pro Thr Leu Leu Pro Leu Asp Thr Lys Val Ser Val 145 150 155 160 Pro Leu Phe Cys Lys Cys Pro Ser Lys Asn Gln Leu Asn Lys Gly Ile 165 170 175 Lys Tyr Leu Ile Thr Tyr Val Trp Gln Asp Asn Asp Asn Val Thr Leu 180 185 190 Val Ser Ser Lys Phe Gly Ala Ser Gln Val Glu Met Leu Ala Glu Asn 195 200 205 Asn His Asn Phe Thr Ala Ser Thr Asn Arg Ser Val Leu Ile Pro Val 210 215 220 Thr Ser Leu Pro Lys Leu Asp Gln Pro Ser Ser Asn Gly Arg Lys Ser 225 230 235 240 Ser Ser Gln Asn Leu Ala Leu Ile Ile Gly Ile Ser Leu Gly Ser Ala 245 250 255 Phe Phe Ile Leu Val Leu Thr Leu Ser Leu Val Tyr Val Tyr Cys Leu 260 265 270 Lys Met Lys Arg Leu Asn Arg Ser Thr Ser Ser Ser Glu Thr Ala Asp 275 280 285 Lys Leu Leu Ser Gly Val Ser Gly Tyr Val Ser Lys Pro Thr Met Tyr 290 295 300 Glu Ile Asp Ala Ile Met Glu Gly Thr Thr Asn Leu Ser Asp Asn Cys 305 310 315 320 Lys Ile Gly Glu Ser Val Tyr Lys Ala Asn Ile Asp Gly Arg Val Leu 325 330 335 Ala Val Lys Lys Ile Lys Lys Asp Ala Ser Glu Glu Leu Lys Ile Leu 340 345 350 Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Val Ser Ser 355 360 365 Asp Asn Asp Gly Asn Cys Phe Leu Val Tyr Glu Tyr Ala Glu Asn Gly 370 375 380 Ser Leu Glu Glu Trp Leu Phe Ser Glu Ser Ser Lys Thr Ser Asn Ser 385 390 395 400 Val Val Ser Leu Thr Trp Ser Gln Arg Ile Thr Ile Ala Met Asp Val 405 410 415 Ala Ile Gly Leu Gln Tyr Met His Glu His Thr Tyr Pro Arg Ile Ile 420 425 430 His Arg Asp Ile Thr Thr Ser Asn Ile Leu Leu Gly Ser Asn Phe Lys 435 440 445 Ala Lys Ile Ala Asn Phe Gly Met Ala Arg Thr Ser Thr Asn Ser Met 450 455 460 Met Pro Lys Ile Asp Val Phe Ala Phe Gly Val Val Leu Ile Glu Leu 465 470 475 480 Leu Thr Gly Lys Lys Ala Met Thr Thr Lys Glu Asn Gly Glu Val Val 485 490 495 Ile Leu Trp Lys Asp Phe Trp Lys Ile Phe Asp Leu Glu Gly Asn Arg 500 505 510 Glu Glu Arg Leu Arg Lys Trp Met Asp Pro Lys Leu Glu Ser Phe Tyr 515 520 525 Pro Ile Asp Asn Ala Leu Ser Leu Ala Ser Leu Ala Val Asn Cys Thr 530 535 540 Ala Asp Lys Ser Leu Ser Arg Pro Thr Ile Ala Glu Ile Val Leu Cys 545 550 555 560 Leu Ser Leu Leu Asn Gln Pro Ser Ser Glu Pro Met Leu Glu Arg Ser 565 570 575 Leu Thr Ser Gly Leu Asp Ala Glu Ala Thr His Val Val Thr Ser Val 580 585 590 Ile Ala Arg 595 2 595 PRT 百脉根 2 Met Ala Val Phe Phe Leu Thr Ser Gly Ser Leu Ser Leu Phe Leu Ala 1 5 10 15 Leu Thr Leu Leu Phe Thr Asn Ile Ala Ala Arg Ser Glu Lys Ile Ser 20 25 30 Gly Pro Asp Phe Ser Cys Pro Val Asp Ser Pro Pro Ser Cys Glu Thr 35 40 45 Tyr Val Thr Tyr Thr Ala Gln Ser Pro Asn Leu Leu Ser Leu Thr Asn 50 55 60 Ile Ser Asp Ile Phe Asp Ile Ser Pro Leu Ser Ile Ala Arg Ala Ser 65 70 75 80 Asn Ile Asp Ala Gly Lys Asp Lys Leu Val Pro Gly Gln Val Leu Leu 85 90 95 Val Pro Val Thr Cys Gly Cys Ala Gly Asn His Ser Ser Ala Asn Thr 100 105 110 Ser Tyr Gln Ile Gln Leu Gly Asp Ser Tyr Asp Phe Val Ala Thr Thr 115 120 125 Leu Tyr Glu Asn Leu Thr Asn Trp Asn Ile Val Gln Ala Ser Asn Pro 130 135 140 Gly Val Asn Pro Tyr Leu Leu Pro Glu Arg Val Lys Val Val Phe Pro 145 150 155 160 Leu Phe Cys Arg Cys Pro Ser Lys Asn Gln Leu Asn Lys Gly Ile Gln 165 170 175 Tyr Leu Ile Thr Tyr Val Trp Lys Pro Asn Asp Asn Val Ser Leu Val 180 185 190 Ser Ala Lys Phe Gly Ala Ser Pro Ala Asp Ile Leu Thr Glu Asn Arg 195 200 205 Tyr Gly Gln Asp Phe Thr Ala Ala Thr Asn Leu Pro Ile Leu Ile Pro 210 215 220 Val Thr Gln Leu Pro Glu Leu Thr Gln Pro Ser Ser Asn Gly Arg Lys 225 230 235 240 Ser Ser Ile His Leu Leu Val Ile Leu Gly Ile Thr Leu Gly Cys Thr 245 250 255 Leu Leu Thr Ala Val Leu Thr Gly Thr Leu Val Tyr Val Tyr Cys Arg 260 265 270 Arg Lys Lys Ala Leu Asn Arg Thr Ala Ser Ser Ala Glu Thr Ala Asp 275 280 285 Lys Leu Leu Ser Gly Val Ser Gly Tyr Val Ser Lys Pro Asn Val Tyr 290 295 300 Glu Ile Asp Glu Ile Met Glu Ala Thr Lys Asp Phe Ser Asp Glu Cys 305 310 315 320 Lys Val Gly Glu Ser Val Tyr Lys Ala Asn Ile Glu Gly Arg Val Val 325 330 335 Ala Val Lys Lys Ile Lys Glu Gly Gly Ala Asn Glu Glu Leu Lys Ile 340 345 350 Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Val Ser 355 360 365 Ser Gly Tyr Asp Gly Asn Cys Phe Leu Val Tyr Glu Tyr Ala Glu Asn 370 375 380 Gly Ser Leu Ala Glu Trp Leu Phe Ser Lys Ser Ser Gly Thr Pro Asn 385 390 395 400 Ser Leu Thr Trp Ser Gln Arg Ile Ser Ile Ala Val Asp Val Ala Val 405 410 415 Gly Leu Gln Tyr Met His Glu His Thr Tyr Pro Arg Ile Ile His Arg 420 425 430 Asp Ile Thr Thr Ser Asn Ile Leu Leu Asp Ser Asn Phe Lys Ala Lys 435 440 445 Ile Ala Asn Phe Ala Met Ala Arg Thr Ser Thr Asn Pro Met Met Pro 450 455 460 Lys Ile Asp Val Phe Ala Phe Gly Val Leu Leu Ile Glu Leu Leu Thr 465 470 475 480 Gly Arg Lys Ala Met Thr Thr Lys Glu Asn Gly Glu Val Val Met Leu 485 490 495 Trp Lys Asp Met Trp Glu Ile Phe Asp Ile Glu Glu Asn Arg Glu Glu 500 505 510 Arg Ile Arg Lys Trp Met Asp Pro Asn Leu Glu Ser Phe Tyr His Ile 515 520 525 Asp Asn Ala Leu Ser Leu Ala Ser Leu Ala Val Asn Cys Thr Ala Asp 530 535 540 Lys Ser Leu Ser Arg Pro Ser Met Ala Glu Ile Val Leu Ser Leu Ser 545 550 555 560 Phe Leu Thr Gln Gln Ser Ser Asn Pro Thr Leu Glu Arg Ser Leu Thr 565 570 575 Ser Ser Gly Leu Asp Val Glu Asp Asp Ala His Ile Val Thr Ser Ile 580 585 590 Thr Ala Arg 595 3 594 PRT 豌豆 3 Met Ala Ile Phe Phe Leu Pro Ser Ser Ser His Ala Leu Phe Leu Ala 1 5 10 15 Leu Met Phe Phe Val Thr Asn Ile Ser Ala Gln Pro Leu Gln Leu Ser 20 25 30 Gly Thr Asn Phe Ser Cys Pro Val Asp Ser Pro Pro Ser Cys Glu Thr 35 40 45 Tyr Val Thr Tyr Phe Ala Arg Ser Pro Asn Phe Leu Ser Leu Thr Asn 50 55 60 Ile Ser Asp Ile Phe Asp Met Ser Pro Leu Ser Ile Ala Lys Ala Ser 65 70 75 80 Asn Ile Glu Asp Glu Asp Lys Lys Leu Val Glu Gly Gln Val Leu Leu 85 90 95 Ile Pro Val Thr Cys Gly Cys Thr Arg Asn Arg Tyr Phe Ala Asn Phe 100 105 110 Thr Tyr Thr Ile Lys Leu Gly Asp Asn Tyr Phe Ile Val Ser Thr Thr 115 120 125 Ser Tyr Gln Asn Leu Thr Asn Tyr Val Glu Met Glu Asn Phe Asn Pro 130 135 140 Asn Leu Ser Pro Asn Leu Leu Pro Pro Glu Ile Lys Val Val Val Pro 145 150 155 160 Leu Phe Cys Lys Cys Pro Ser Lys Asn Gln Leu Ser Lys Gly Ile Lys 165 170 175 His Leu Ile Thr Tyr Val Trp Gln Ala Asn Asp Asn Val Thr Arg Val 180 185 190 Ser Ser Lys Phe Gly Ala Ser Gln Val Asp Met Phe Thr Glu Asn Asn 195 200 205 Gln Asn Phe Thr Ala Ser Thr Asn Val Pro Ile Leu Ile Pro Val Thr 210 215 220 Lys Leu Pro Val Ile Asp Gln Pro Ser Ser Asn Gly Arg Lys Asn Ser 225 230 235 240 Thr Gln Lys Pro Ala Phe Ile Ile Gly Ile Ser Leu Gly Cys Ala Phe 245 250 255 Phe Val Val Val Leu Thr Leu Ser Leu Val Tyr Val Tyr Cys Leu Lys 260 265 270 Met Lys Arg Leu Asn Arg Ser Thr Ser Leu Ala Glu Thr Ala Asp Lys 275 280 285 Leu Leu Ser Gly Val Ser Gly Tyr Val Ser Lys Pro Thr Met Tyr Glu 290 295 300 Met Asp Ala Ile Met Glu Ala Thr Met Asn Leu Ser Glu Asn Cys Lys 305 310 315 320 Ile Gly Glu Ser Val Tyr Lys Ala Asn Ile Asp Gly Arg Val Leu Ala 325 330 335 Val Lys Lys Ile Lys Lys Asp Ala Ser Glu Glu Leu Lys Ile Leu Gln 340 345 350 Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Val Ser Ser Asp 355 360 365 Asn Asp Gly Asn Cys Phe Leu Val Tyr Glu Tyr Ala Glu Asn Gly Ser 370 375 380 Leu Asp Glu Trp Leu Phe Ser Glu Ser Ser Lys Thr Ser Asn Ser Val 385 390 395 400 Val Ser Leu Thr Trp Ser Gln Arg Ile Thr Val Ala Val Asp Val Ala 405 410 415 Val Gly Leu Gln Tyr Met His Glu His Thr Tyr Pro Arg Ile Ile His 420 425 430 Arg Asp Ile Thr Thr Ser Asn Ile Leu Leu Asp Ser Asn Phe Lys Ala 435 440 445 Lys Ile Ala Asn Phe Ser Met Ala Arg Thr Ser Thr Asn Ser Met Met 450 455 460 Pro Lys Ile Asp Val Phe Ala Phe Gly Val Val Leu Ile Glu Leu Leu 465 470 475 480 Thr Gly Lys Lys Ala Ile Thr Thr Met Glu Asn Gly Glu Val Val Ile 485 490 495 Leu Trp Lys Asp Phe Trp Lys Ile Phe Asp Leu Glu Gly Asn Arg Glu 500 505 510 Glu Ser Leu Arg Lys Trp Met Asp Pro Lys Leu Glu Asn Phe Tyr Pro 515 520 525 Ile Asp Asn Ala Leu Ser Leu Ala Ser Leu Ala Val Asn Cys Thr Ala 530 535 540 Asp Lys Ser Leu Ser Arg Pro Ser Ile Ala Glu Ile Val Leu Cys Leu 545 550 555 560 Ser Leu Leu Asn Gln Ser Ser Ser Glu Pro Met Leu Glu Arg Ser Leu 565 570 575 Thr Ser Gly Leu Asp Val Glu Ala Thr His Val Val Thr Ser Ile Val 580 585 590 Ala Arg 4 598 PRT 大豆 4 Met Ala Val Phe Phe Pro Phe Leu Pro Leu His Ser Gln Ile Leu Cys 1 5 10 15 Leu Val Ile Met Leu Phe Ser Thr Asn Ile Val Ala Gln Ser Gln Gln 20 25 30 Asp Asn Arg Thr Asn Phe Ser Cys Pro Ser Asp Ser Pro Pro Ser Cys 35 40 45 Glu Thr Tyr Val Thr Tyr Ile Ala Gln Ser Pro Asn Phe Leu Ser Leu 50 55 60 Thr Asn Ile Ser Asn Ile Phe Asp Thr Ser Pro Leu Ser Ile Ala Arg 65 70 75 80 Ala Ser Asn Leu Glu Pro Met Asp Asp Lys Leu Val Lys Asp Gln Val 85 90 95 Leu Leu Val Pro Val Thr Cys Gly Cys Thr Gly Asn Arg Ser Phe Ala 100 105 110 Asn Ile Ser Tyr Glu Ile Asn Gln Gly Asp Ser Phe Tyr Phe Val Ala 115 120 125 Thr Thr Ser Tyr Glu Asn Leu Thr Asn Trp Arg Ala Val Met Asp Leu 130 135 140 Asn Pro Val Leu Ser Pro Asn Lys Leu Pro Ile Gly Ile Gln Val Val 145 150 155 160 Phe Pro Leu Phe Cys Lys Cys Pro Ser Lys Asn Gln Leu Asp Lys Glu 165 170 175 Ile Lys Tyr Leu Ile Thr Tyr Val Trp Lys Pro Gly Asp Asn Val Ser 180 185 190 Leu Val Ser Asp Lys Phe Gly Ala Ser Pro Glu Asp Ile Met Ser Glu 195 200 205 Asn Asn Tyr Gly Gln Asn Phe Thr Ala Ala Asn Asn Leu Pro Val Leu 210 215 220 Ile Pro Val Thr Arg Leu Pro Val Leu Ala Arg Ser Pro Ser Asp Gly 225 230 235 240 Arg Lys Gly Gly Ile Arg Leu Pro Val Ile Ile Gly Ile Ser Leu Gly 245 250 255 Cys Thr Leu Leu Val Leu Val Leu Ala Val Leu Leu Val Tyr Val Tyr 260 265 270 Cys Leu Lys Met Lys Thr Leu Asn Arg Ser Ala Ser Ser Ala Glu Thr 275 280 285 Ala Asp Lys Leu Leu Ser Gly Val Ser Gly Tyr Val Ser Lys Pro Thr 290 295 300 Met Tyr Glu Thr Asp Ala Ile Met Glu Ala Thr Met Asn Leu Ser Glu 305 310 315 320 Gln Cys Lys Ile Gly Glu Ser Val Tyr Lys Ala Asn Ile Glu Gly Lys 325 330 335 Val Leu Ala Val Lys Arg Phe Lys Glu Asp Val Thr Glu Glu Leu Lys 340 345 350 Ile Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Val 355 360 365 Ser Ser Asp Asn Asp Gly Asn Cys Phe Val Val Tyr Glu Tyr Ala Glu 370 375 380 Asn Gly Ser Leu Asp Glu Trp Leu Phe Ser Lys Ser Cys Ser Asp Thr 385 390 395 400 Ser Asn Ser Arg Ala Ser Leu Thr Trp Cys Gln Arg Ile Ser Met Ala 405 410 415 Val Asp Val Ala Met Gly Leu Gln Tyr Met His Glu His Ala Tyr Pro 420 425 430 Arg Ile Val His Arg Asp Ile Thr Ser Ser Asn Ile Leu Leu Asp Ser 435 440 445 Asn Phe Lys Ala Lys Ile Ala Asn Phe Ser Met Ala Arg Thr Phe Thr 450 455 460 Asn Pro Met Met Pro Lys Ile Asp Val Phe Ala Phe Gly Val Val Leu 465 470 475 480 Ile Glu Leu Leu Thr Gly Arg Lys Ala Met Thr Thr Lys Glu Asn Gly 485 490 495 Glu Val Val Met Leu Trp Lys Asp Ile Trp Lys Ile Phe Asp Gln Glu 500 505 510 Glu Asn Arg Glu Glu Arg Leu Lys Lys Trp Met Asp Pro Lys Leu Glu 515 520 525 Ser Tyr Tyr Pro Ile Asp Tyr Ala Leu Ser Leu Ala Ser Leu Ala Val 530 535 540 Asn Cys Thr Ala Asp Lys Ser Leu Ser Arg Pro Thr Ile Ala Glu Ile 545 550 555 560 Val Leu Ser Leu Ser Leu Leu Thr Gln Pro Ser Pro Ala Thr Leu Glu 565 570 575 Arg Ser Leu Thr Ser Ser Gly Leu Asp Val Glu Ala Thr Gln Ile Val 580 585 590 Thr Ser Ile Ala Ala Arg 595 5 557 PRT 鹰嘴豆 5 Met Ser Val Phe Phe Leu Pro Ser Arg Ser His Val Leu Phe Leu Ala 1 5 10 15 Leu Met Leu Phe Leu Thr Asn Ile Ser Ala Gln Ser Gln His Leu Ser 20 25 30 Gly Thr Asn Phe Ser Cys Pro Val Asp Ser Pro Pro Ser Cys Glu Thr 35 40 45 Tyr Val Thr Tyr Ile Ala Gln Ser Pro Asn Phe Leu Ser Leu Thr Asn 50 55 60 Ile Ser Asp Leu Phe Asp Ile Ser Pro Leu Ser Ile Ala Arg Ala Ser 65 70 75 80 Asn Ile Asp Asp Glu Asp Lys Glu Leu Ile Pro Gly Gln Val Leu Leu 85 90 95 Val Pro Val Thr Cys Gly Cys Thr Lys His Arg Ser Phe Ala Asn Asn 100 105 110 Thr Tyr Thr Ile Lys Leu Gly Asp Ser Tyr Ile Leu Val Ser Thr Thr 115 120 125 Ser Tyr Gln Asn Leu Thr Asn Tyr Leu Glu Met Glu Asp Ser Asn Pro 130 135 140 Gly Leu Asn Pro Asn Leu Ile Pro Pro Phe Ile Lys Val Val Val Pro 145 150 155 160 Ile Phe Cys Arg Cys Pro Ser Lys Thr Gln Leu Asn Lys Gly Ile Lys 165 170 175 Tyr Leu Ile Thr Tyr Val Trp His Ala Asn Asp Asn Val Ser Thr Val 180 185 190 Ser Ser Lys Phe Gly Ala Ser Gln Val Asp Ile Leu Thr Glu Asn Asn 195 200 205 Tyr Asn Gln Asn Phe Ala Ser Ala Ala Asn Leu Pro Val Leu Ile Pro 210 215 220 Val Thr Arg Leu Pro Ile Leu Ala Gln Pro Ser Ser Asn Gly Arg Lys 225 230 235 240 Arg Ser Ile Gln Leu Pro Val Ile Ile Asp Lys Leu Leu Ser Gly Val 245 250 255 Ser Gly Tyr Val Ser Lys Pro Thr Met Tyr Glu Met Asp Val Ile Met 260 265 270 Glu Ala Thr Met Asn Leu Ser Asp Gln Cys Lys Ile Gly Glu Ser Val 275 280 285 Tyr Lys Ala Asn Ile Asp Gly Lys Val Leu Ala Val Lys Lys Thr Lys 290 295 300 Lys Asp Ala Ser Glu Glu Leu Lys Ile Leu Gln Lys Val Asn His Gly 305 310 315 320 Asn Leu Val Lys Leu Met Gly Val Ser Ser Asp Asn Glu Gly Asn Cys 325 330 335 Phe Leu Val Tyr Glu Tyr Ala Glu Asn Gly Ser Leu Asp Glu Trp Leu 340 345 350 Phe Leu Glu Ser Ser Lys Thr Ser Asp Ser Thr Val Ser Leu Thr Trp 355 360 365 Ser Gln Arg Ile Gly Ile Ala Val Asp Val Ala Val Gly Leu Gln Tyr 370 375 380 Met His Glu His Thr Tyr Pro Arg Ile Ile His Arg Asp Ile Thr Thr 385 390 395 400 Ser Asn Ile Leu Leu Asp Ala Asn Phe Lys Ala Lys Ile Ala Asn Phe 405 410 415 Ser Met Ala Arg Thr Ser Thr Asn Pro Met Met Pro Lys Ile Asp Val 420 425 430 Phe Ala Phe Gly Val Val Leu Ile Glu Leu Leu Thr Gly Lys Lys Gly 435 440 445 Val Thr Thr Lys Glu Asn Gly Glu Val Val Ile Met Trp Lys Asp Phe 450 455 460 Trp Met Ile Phe Asp Leu Glu Gly Asn Lys Glu Glu Arg Leu Arg Lys 465 470 475 480 Trp Met Asp Pro Lys Leu Glu Asn Phe Tyr Pro Ile Asp Asn Ala Leu 485 490 495 Ser Leu Ala Ser Leu Ala Val Asn Cys Thr Ala Asp Lys Ser Leu Ser 500 505 510 Arg Pro Thr Ile Glu Glu Ile Val Leu Cys Leu Asn Leu Leu Asn Gln 515 520 525 Pro Ser Ser Glu Pro Thr Leu Glu Arg Ser Leu Thr Phe Gly Leu Asp 530 535 540 Val Glu Asp Thr Gln Ile Val Thr Ser Ile Ala Ala Arg 545 550 555 6 2144 DNA 鹰嘴豆 6 ttgaaccttc gagctcttgc atataactta ttcaatgtca ctagccaaat tactactgag 60 atttaaacat aagtaatcta tttttttata tgacaggact ttttgataga ggaagaaaat 120 gattggtatg cgaaaagata ttttattgac aacaaatgct ccatatcacc aatgctaagt 180 tcctgcatta agaaacactc ttctctcttc ccctcataat aacttccatt tcttcacaac 240 tttcacaaaa tgtctgtctt ctttcttccc tctagatctc atgttctttt tcttgcactc 300 atgttgtttc tcactaacat ctcagctcaa tcacaacacc tcagtggaac aaacttttca 360 tgccctgtgg attcacctcc ttcatgtgaa acttatgtga catacattgc tcagtctcca 420 aattttttaa gcctaacaaa catatctgat ttatttgata tcagtccttt atccatcgca 480 agagcgagta acatagacga cgaggataaa gagctgatac caggtcaagt cttattagta 540 cctgtaactt gtggctgcac taaacatcgc tctttcgcca ataacaccta cacgatcaag 600 ctcggcgaca gctacattct agtttcaacc acttcatatc agaatctcac caattatctt 660 gaaatggaag attccaaccc tggtctaaat cctaatctta ttccaccatt catcaaagtt 720 gtagtcccaa tattctgcag gtgcccttca aagactcagc tgaacaaagg aataaagtat 780 ctgataactt acgtgtggca cgctaacgac aatgtttcaa ctgtaagttc caaatttggt 840 gcatcacaag ttgatatatt gactgaaaac aattacaatc aaaactttgc ttctgcagcc 900 aaccttccag ttttgattcc tgtgacaagg ttacctattc tagctcaacc gtcttcgaat 960 ggaagaaaga gaagcattca acttcctgtt ataattggta ttagtatagg aagtgctttt 1020 ttcgttacag ttttaacagt atcacttgtg tatttatact gtctgaaaat gaagagattg 1080 aataggactg cttctttatc tgagactgca gataagttac tttcaggagt ttcgggttac 1140 gtaagcaagc caacaatgta tgaaatggat gtgatcatgg aagctacaat gaacctgagt 1200 gaccaatgta agattggtga atcagtttat aaggctaata tagatggtaa agttttagca 1260 gtgaaaaaaa ctaagaaaga tgcttctgag gagctgaaaa ttctgcagaa ggtaaatcat 1320 ggaaatctgg tgaaactaat gggtgtgtct tcggacaacg agggtaactg ttttctggtt 1380 tatgagtatg ctgaaaatgg ttctcttgat gaatggttgt tcttggaatc ttcgaaaact 1440 tcggattcga cagtctccct tacatggtct cagagaatag gcatagcagt ggatgttgca 1500 gttggtctgc aatacatgca tgaacatact tatccaagga taatccacag agacatcaca 1560 acaagtaata tccttctcga cgcgaacttt aaggccaaga tagcgaattt ttcgatggct 1620 agaacttcaa ccaacccgat gatgccgaaa atagatgttt tcgcttttgg ggtggttctg 1680 atagagttgc taaccggaaa aaaaggcgta acaacgaaag aaaatggtga ggttgttatt 1740 atgtggaagg atttttggat gatttttgat ctagaaggga ataaagaaga gaggctaaga 1800 aaatggatgg atcctaagtt agaaaacttt tatcctatag ataatgctct tagtttggct 1860 tctttggcag tgaattgcac tgctgataaa tcattgtcaa gaccaactat tgaagaaatt 1920 gttctttgtc ttaaccttct caatcaacca tcatctgaac caacattaga aagatctttg 1980 acatttgggt tagatgttga agatactcaa attgttactt ctatagcagc tcgttgatca 2040 agtgaagata atattaattc tgttttcttt catattgaag atggtacttt gtttacatga 2100 taactatatt tttatgcgtg gaagtatatg gttagtttaa ttaa 2144 7 597 PRT 菜豆 7 Met Ala Val Phe Phe Val Ser Leu Thr Leu Gly Ala Gln Ile Leu Tyr 1 5 10 15 Val Val Leu Met Phe Phe Thr Cys Ile Glu Ala Gln Ser Gln Gln Thr 20 25 30 Asn Gly Thr Asn Phe Ser Cys Pro Ser Asn Ser Pro Pro Ser Cys Glu 35 40 45 Thr Tyr Val Thr Tyr Ile Ser Gln Ser Pro Asn Phe Leu Ser Leu Thr 50 55 60 Ser Val Ser Asn Ile Phe Asp Thr Ser Pro Leu Ser Ile Ala Arg Ala 65 70 75 80 Ser Asn Leu Gln His Glu Glu Asp Lys Leu Ile Pro Gly Gln Val Leu 85 90 95 Leu Ile Pro Val Thr Cys Gly Cys Thr Gly Asn Arg Ser Phe Ala Asn 100 105 110 Ile Ser Tyr Glu Ile Asn Gln Gly Asp Ser Phe Tyr Phe Val Ala Thr 115 120 125 Thr Leu Tyr Gln Asn Leu Thr Asn Trp His Ala Val Met Asp Leu Asn 130 135 140 Pro Gly Leu Ser Pro Phe Thr Leu Pro Ile Gly Ile Gln Val Val Ile 145 150 155 160 Pro Leu Phe Cys Lys Cys Pro Ser Lys Asn Gln Leu Asp Arg Gly Ile 165 170 175 Lys Tyr Leu Ile Thr His Val Trp Gln Pro Asn Asp Asn Val Ser Phe 180 185 190 Val Ser Asn Lys Leu Gly Ala Ser Pro Gln Asp Ile Leu Ser Glu Asn 195 200 205 Asn Tyr Gly Gln Asn Phe Thr Ala Ala Ser Asn Leu Pro Val Leu Ile 210 215 220 Pro Val Thr Leu Leu Pro Asp Leu Ile Gln Ser Pro Ser Asp Gly Arg 225 230 235 240 Lys His Arg Ile Gly Leu Pro Val Ile Ile Gly Ile Ser Leu Gly Cys 245 250 255 Thr Leu Leu Val Val Val Ser Ala Ile Leu Leu Val Cys Val Cys Cys 260 265 270 Leu Lys Met Lys Ser Leu Asn Arg Ser Ala Ser Ser Ala Glu Thr Ala 275 280 285 Asp Lys Leu Leu Ser Gly Val Ser Gly Tyr Val Ser Lys Pro Thr Met 290 295 300 Tyr Glu Thr Gly Ala Ile Leu Glu Ala Thr Met Asn Leu Ser Glu Gln 305 310 315 320 Cys Lys Ile Gly Glu Ser Val Tyr Lys Ala Asn Ile Glu Gly Lys Val 325 330 335 Leu Ala Val Lys Arg Phe Lys Glu Asp Val Thr Glu Glu Leu Lys Ile 340 345 350 Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Val Ser 355 360 365 Ser Asp Asn Asp Gly Asn Cys Phe Val Val Tyr Glu Tyr Ala Glu Asn 370 375 380 Gly Ser Leu Gln Glu Trp Leu Phe Ala Lys Ser Cys Ser Glu Thr Leu 385 390 395 400 Asn Ser Arg Thr Ser Leu Thr Trp Cys Gln Arg Ile Ser Ile Ala Val 405 410 415 Asp Val Ser Met Gly Leu Gln Tyr Met His Glu His Ala Tyr Pro Arg 420 425 430 Ile Val His Arg Asp Ile Thr Ser Ser Asn Ile Leu Leu Asp Ser Asn 435 440 445 Phe Lys Ala Lys Ile Ala Asn Phe Ser Met Ala Arg Thr Phe Thr Asn 450 455 460 Pro Met Met Ser Lys Ile Asp Val Phe Ala Phe Gly Val Val Leu Ile 465 470 475 480 Glu Leu Leu Thr Gly Arg Lys Ala Met Thr Thr Lys Glu Asn Gly Glu 485 490 495 Val Val Met Leu Trp Thr Asp Ile Trp Lys Ile Phe Asp Gln Glu Glu 500 505 510 Asn Arg Glu Glu Arg Leu Arg Lys Trp Met Asp Pro Lys Leu Asp Asn 515 520 525 Tyr Tyr Pro Ile Asp Tyr Ala Leu Ser Leu Ala Ser Leu Ala Met Asn 530 535 540 Cys Thr Ala Asp Lys Ser Leu Ser Arg Pro Thr Ile Ala Glu Ile Val 545 550 555 560 Leu Ser Leu Ser Leu Leu Thr Gln Pro Ser Pro Ala Thr Leu Glu Arg 565 570 575 Ser Leu Thr Ser Ser Gly Leu Asp Val Glu Ala Thr Gln Ile Val Thr 580 585 590 Ser Ile Ser Ala Arg 595 8 2192 DNA 菜豆 8 ggaaatttag ttaaagctaa tgacacaaac aggaccatat ttttatatta agccaaaaga 60 tatttttatt gacaaagaac tacatatcaa caacgacgat tgccagtgat agtagactgc 120 ctcataactt tcatttgttc acaacttcac atcaatggct gtcttctttg tttctcttac 180 tcttggtgct cagattcttt atgtggtact catgtttttc acttgtattg aagctcaatc 240 acaacagacc aatggaacaa acttttcatg cccttccaat tcacctcctt catgtgaaac 300 ctatgtgaca tacatatccc agtcgccaaa ttttttgagt ctgaccagcg tatctaatat 360 atttgacacg agtcctttgt caattgccag agccagcaac ttacagcatg aggaagacaa 420 gttgattcca ggccaagtct tactgatacc agtaacctgt ggttgcactg gaaaccgctc 480 tttcgccaac atctcctatg agatcaacca aggtgatagc ttctactttg ttgcgaccac 540 tttataccag aatctcacaa attggcatgc agtgatggat ttaaacccag gtctaagtcc 600 atttactttg ccaataggca tccaagttgt aattccttta ttctgcaagt gtccttcaaa 660 gaaccagctg gatagaggga taaagtacct gatcactcac gtctggcagc ccaatgacaa 720 tgtttccttt gtaagtaaca agttaggtgc atcaccacag gacatattga gtgaaaacaa 780 ctatggtcaa aacttcactg ccgcaagcaa ccttccagtt ttgatcccag ttacactctt 840 gccagatctt attcaatctc cttcagatgg aagaaaacac agaattggtc ttccagttat 900 aattggtatc agcctgggat gcacactact ggttgtggtt tcagcaatat tactggtgtg 960 tgtatgttgt ctgaaaatga agagtttgaa taggagtgct tcatcagctg aaactgcaga 1020 taaactactt tctggagttt caggctatgt aagtaagcct acaatgtatg aaactggtgc 1080 aatattggaa gctactatga acctcagtga gcagtgcaag attggggaat cagtgtacaa 1140 ggctaacata gagggtaagg ttttagcagt aaaaagattc aaggaagatg tcacggagga 1200 gctgaaaatt ctgcagaagg tgaatcatgg aaatctggtg aaactaatgg gtgtctcatc 1260 agataatgat ggaaattgtt ttgtggttta tgaatatgct gaaaatgggt ctcttcaaga 1320 gtggcttttc gccaagtctt gttcagagac attaaactcg aggacctccc ttacatggtg 1380 ccagaggata agcatagcag tggatgtttc aatgggtctg cagtacatgc atgaacatgc 1440 ttatccaaga atagtccaca gggacatcac aagcagtaat atccttcttg actccaactt 1500 taaggccaag atagcaaatt tctccatggc cagaactttt accaacccca tgatgtcaaa 1560 aatagatgta tttgcttttg gggtggttct gatagaattg cttactggca ggaaagccat 1620 gacaaccaaa gaaaatggtg aggtggttat gctgtggacg gacatttgga agatctttga 1680 tcaagaagag aatagagagg agaggctcag aaaatggatg gatcctaagt tagataatta 1740 ttatcctatt gattatgctc tcagcttggc ctccttggca atgaattgca ctgcagacaa 1800 gtctttgtcc agaccaacca tagcagaaat tgtccttagt ctctcccttc tcactcaacc 1860 atctcccgcg acactggaga gatccttgac ttcttctgga ttagatgtag aagctactca 1920 aattgtcact tccatctcag ctcgttgatt gagtgaagcc aatctagttt ctcacatcca 1980 agatggtact tttttttaaa taatgattgc accttagtca ataatgatga acttggggag 2040 ttttcaacat ttagtgtttc catccctgtt gttctttatg tttgaggtag agttcgtaaa 2100 acgaatagca attgcagttc tcctcagact aaatttgctt atttctctgt acttctttta 2160 tatgacaatt gaaagtgaat caaatgatgg ag 2192 9 595 PRT 花生亚种花生 9 Met Ala Phe Phe Leu Pro Ser Leu Ser Ser Ser Ile Phe Leu Ala Phe 1 5 10 15 Met Leu Phe Ser Val Thr Ser Ile Pro Thr Gln Ser Gln Gln Val Asn 20 25 30 Gly Thr Asp Phe Ser Cys Pro Val Asp Ser Pro Ser Ser Cys Gly Thr 35 40 45 Tyr Val Thr Tyr Ile Ala Lys Ser Pro Asn Phe Leu Ser Leu Ser Asn 50 55 60 Ile Ser Asp Ile Phe Asp Thr Ser Pro Leu Ser Ile Ala Arg Ala Ser 65 70 75 80 Asn Ile Lys Asn Glu Gly Asp Lys Leu Val Pro Gly Gln Val Leu Leu 85 90 95 Ile Pro Val Thr Cys Gly Cys Thr Gln Asn Gln Ser Phe Ala Asn Ile 100 105 110 Thr Tyr Glu Leu Arg Gln Gly Asp Val Tyr Asp Ile Val Ser Lys Thr 115 120 125 Thr Tyr Glu Asn Leu Thr Asn Trp Arg Ala Val Asn Asn Ser Asn Pro 130 135 140 Asp Leu Asn Pro Val Leu Leu Pro Ile Gly Val Lys Val Leu Phe Pro 145 150 155 160 Leu Phe Cys Arg Cys Pro Ser Lys Lys Gln Leu Gln Lys Gly Ile Glu 165 170 175 Tyr Met Ile Thr Tyr Val Trp Gln Asn Asn Asp Asn Val Ser Ser Val 180 185 190 Ala Ala Lys Phe Gly Ala Ser Pro Val Asp Ile Leu Ser Glu Asn Asn 195 200 205 Tyr Gly Gly Asn Phe Thr Ala Ala Thr Tyr Leu Pro Val Leu Ile Pro 210 215 220 Val Thr Lys Leu Pro Val Leu Thr Gln Pro Glu Ala Ser His Gly Arg 225 230 235 240 Lys Arg Ser Ile Gln Ile Pro Val Ile Ile Ser Ile Ser Leu Gly Phe 245 250 255 Thr Leu Val Val Ala Val Ile Val Ile Ser Met Val Tyr Ala Tyr Leu 260 265 270 Tyr Gln Arg Lys Arg Thr Leu Asn Arg Gly Asp Leu Ser Ala Gly Thr 275 280 285 Ala Asp Lys Leu Leu Ser Gly Val Ser Gly Tyr Val Ser Lys Pro Thr 290 295 300 Val Tyr Glu Ala Asn Glu Val Ile Lys Ala Thr Met Asn Leu Ser Gly 305 310 315 320 Gln Cys Lys Leu Gly Gly Thr Val Tyr Lys Ala Lys Ile Glu Gly Gln 325 330 335 Val Leu Ala Val Lys Lys Val Asn Gln Val Val Ser Glu Glu Leu Asn 340 345 350 Ile Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Val 355 360 365 Ser Ser Asp Ser Asp Gly Asn His Phe Leu Val Tyr Glu Tyr Ala Asp 370 375 380 Asn Gly Ser Leu Asp Gly Trp Leu Phe Ser Lys Leu Ser Leu Lys Ala 385 390 395 400 Ser Leu Thr Trp Tyr Gln Arg Ile Asn Ile Ala Leu Asp Val Ala Met 405 410 415 Gly Leu Gln Tyr Leu His Glu His Thr Tyr Pro Arg Ile Val His Arg 420 425 430 Asp Ile Thr Thr Ser Asn Ile Leu Leu Asp Ser Asn Phe Lys Ala Lys 435 440 445 Ile Gly Asn Phe Ser Met Val Arg Thr Thr Thr Asn Pro Met Ile Ser 450 455 460 Lys Ile Asp Val Phe Ala Phe Gly Ala Val Leu Ile Glu Leu Leu Thr 465 470 475 480 Gly Met Lys Ala Met Thr Thr Lys Ala Asp Gly Glu Val Val Met Leu 485 490 495 Trp Lys Asp Ile Arg Lys Met Phe Glu Val Glu Asp Glu Lys Glu Lys 500 505 510 Glu Glu Cys Leu Arg Arg Trp Met Asp Pro Lys Leu Glu Cys Leu Tyr 515 520 525 Pro Val Asp Tyr Ala Leu Ser Leu Ala Thr Leu Ala Ala Asn Cys Thr 530 535 540 Ala Asp Val Ser Leu Ser Arg Pro Thr Met Ala Glu Val Val Leu Gly 545 550 555 560 Leu Ser Leu Leu Thr Gln Pro Ser Gln Ala Ala Leu Glu Arg Ser Leu 565 570 575 Thr Ser Ser Ala Leu Glu Ala Glu Val Thr His Val Ala Thr Ser Ile 580 585 590 Thr Ala Arg 595 10 2683 DNA 花生亚种花生 10 atctttctct taacagtagt agctagatta cagaatctct tattctattg ttttcgatta 60 gattagatgg aatcacgtat atgaaccgac accgtgaagc atgaaagccg cactcaaata 120 ttcaagaagg ctatttcaaa aatatgaaac cagggaaaaa atgcaagtgt aaaataatgc 180 ttatgaaaaa ttgtatgcag tgtaggtagt aattcttata aaattcatta gacccgaata 240 cttttagcac gtataaatct tgtggagaca ctacatttta aggcgtggtc tagtgataga 300 tagacatgga attggcatca gaattttctc ttatgatgtc tcaaattgga gattctttgc 360 atataagtta aaaatgacca tcctggaatc ttcattggca ttttattata ctctgctgtg 420 cgcatgaata gcatactatt gatatatgta aagtcaatgt tttccttaac atataatctt 480 agacttactt ggaaatttga tatcactgca taaataatta ggaatagata tggtaaaaca 540 gtataccaat aactcttctg ttaaaccaaa gggtatttta ttgacaaaga atcctccata 600 tcaccaaaaa tcctgagtgt ctttgacttc tgataataaa agtttccttt atgttctttc 660 cctccttctc aacttcagaa aaatggcttt ctttctaccc tctctctcaa gtagtatttt 720 tcttgtattc atgttctcca tcaccagcat cccaactcaa tcacaacagg ttaatggaac 780 agacttttca tgcccagtgg attcaccttc ttcctgtgga acatatgtga catacatcgc 840 taaatctcca aacttcttga gcctttctaa catatctgac atatttgaca ccagcccttt 900 atccattgca agagcaagta acataaagaa tgagggtgac aagctggttc caggccaagt 960 cttactgata cctgtcactt gtggttgcac tcaaaaccaa tctttcgcca atatcaccta 1020 tgagctaagg cagggtgata tgtacgactt tgtctcaaaa acaacatatg agaatctcac 1080 aaattggcgt gctgtcaacg attcaaaccc agatttgaat ccagttctgc tgccagtagg 1140 tgtgaaagta ttgttccctt tattctgcag gtgcccttct aagaagcagt tacaaaaagg 1200 gatagaatat atgatcacct atgtgtggca gaacaatgac aatgtttcct ctgtagcagc 1260 caagtttggt gcatcggcag tggacatatt gtccgaaaac aactatggtg gaaacttcac 1320 agctgcaacc tatcttccgg ttttgattcc tgtgacgaag ttgccggttc ttactcaacc 1380 cgagccttca catggaagaa agagaagcat tcaaatccct gttataatca gtattagcct 1440 ggggttcacc cttgttgttg ctgttatagt aatatcaatg gtttatgctt atctttatca 1500 gagaaagagg actttgaata ggagagactc atctgctggg acagcagata agctactctc 1560 tggagtctca ggctacgtga gtaagccaac cgtgtatgaa gccaatgagg ttatcaaagc 1620 caccatgaat ctcagcgaac agtgcaagct tgggggcaca gtttacaagg ccaaaataga 1680 agggcaggtc ttggcagtga aaaaagtgaa tcaagtagtt tctgaggagc tgaatattct 1740 gcagaaggtg aatcatggaa acctggtgaa actgatgggt gtatcttcag acagtgatgg 1800 aaaccatttc ctggtttatg agtatgctga taacgggtcc cttgatgggt ggctcttctc 1860 caagttgtct ttgaaggcct cgcttacatg gtatcagagg attaacatag cattggatgt 1920 tgccatgggt ctgcaatact tgcatgagca cacttatcca agaatagtcc atagggacat 1980 cacaacaagt aacatccttc ttgactccaa cttcaaggcc aagataggga acttctccat 2040 ggtcagaact actacaaatc ccatgatttc caagatcgat gtctttgctt tcggggttgt 2100 tctgattgag ttgcttacag gcaggaaagc catgacaaca aaggcagatg gtgaggtagt 2160 aatgctgtgg aaggatatta ggaagatgtt tgaagtggaa gatgaaaagg aaaaggagga 2220 atgtctgaga agatggatgg atcctaagct agagtgcctt taccctgtgg attatgctct 2280 cagcttggcc acgttggccg cgaattgcac ggcggatgta tcattgtcta gaccaaccat 2340 ggcagaagtt gttcttggcc tctcccttct cactcaacca tctcaagctg cactagagag 2400 atcattgact tcttctgcgt tggaagcaga ggttactcat gtggctactc ccatagcagc 2460 acgttaattg gtgacgaaag taattcagtt tctcaggttc aagagagtgt tttcttcaca 2520 tgactactgc ctataagctt tgttaataag tgtccaagtt tattgtcgct ttaaagtttc 2580 tttgtttcat cccttatatt ctttatctgt ttgtagtcga ggaatgactg cttatttctc 2640 tcaagttgac atatatgata tatataacaa aaagaatatt ttg 2683 11 591 PRT 百脉根 11 Met Thr Ser Phe Phe Leu Phe Thr Asn Thr Leu Phe Leu Ala Leu Met 1 5 10 15 Met Phe Phe Ser Thr Thr His His Ile Leu Ala Gln Leu Ser His Thr 20 25 30 Asn Gly Thr Asn Phe Ser Cys Pro Val Asp Ser Pro Pro Ser Cys Asp 35 40 45 Thr Tyr Val Thr Tyr Phe Ala Gln Ser Pro Asn Phe Leu Thr Leu Thr 50 55 60 Ser Ile Ser Asp Leu Phe Asp Thr Ser Pro Leu Ser Ile Ala Arg Ala 65 70 75 80 Ser Asn Ile Lys Asp Glu Asn Gln Asn Leu Val Pro Gly Gln Leu Leu 85 90 95 Leu Val Pro Val Thr Cys Ala Cys Ser Gly Ser Asn Ser Phe Ser Asn 100 105 110 Ile Ser His Met Ile Lys Glu Gly Glu Ser Tyr Tyr Tyr Leu Ser Thr 115 120 125 Thr Ser Tyr Glu Asn Leu Thr Asn Trp Glu Thr Val Gln Asp Ser Asn 130 135 140 Pro Asn Tyr Asn Pro Tyr Leu Leu Pro Val Gly Ile Lys Val Val Ile 145 150 155 160 Pro Leu Phe Cys Lys Cys Pro Ser Asn Tyr His Leu Asn Lys Gly Ile 165 170 175 Glu Tyr Leu Ile Thr Tyr Val Trp His Asn Asn Asp Asn Val Ser Leu 180 185 190 Val Ala Ser Lys Phe Gly Val Ser Thr Gln Asp Ile Ile Ser Glu Asn 195 200 205 Asn Phe Ser His Gln Asn Phe Thr Ala Ala Thr Asn Phe Pro Ile Leu 210 215 220 Ile Pro Val Thr Gln Leu Pro Ser Leu Ser Gln Ser Tyr Ser Ser Ser 225 230 235 240 Glu Arg Lys Arg Ser Asn His Ile His Ile Ile Ile Ser Ile Gly Ile 245 250 255 Ser Leu Gly Ser Thr Leu Leu Ile Ala Leu Leu Val Leu Val Ser Val 260 265 270 Thr Cys Leu Arg Lys Arg Lys Ser Ser Glu Asn Lys Ser Leu Leu Ser 275 280 285 Val Glu Ile Ala Gly Lys Lys Leu Ile Ser Gly Val Ser Asn Tyr Val 290 295 300 Ser Lys Ser Ile Leu Tyr Glu Phe Arg Leu Ile Met Glu Ala Thr Leu 305 310 315 320 Asn Leu Asn Glu Gln Cys Lys Ile Gly Glu Ser Val Tyr Lys Ala Lys 325 330 335 Leu Asp Gly Gln Val Leu Ala Val Lys Lys Val Lys Glu Asp Val Thr 340 345 350 Glu Glu Val Met Ile Leu Gln Lys Val Asn His Leu Asn Leu Val Lys 355 360 365 Leu Met Gly Val Ser Ser Gly His Asp Gly Asn His Phe Leu Val Tyr 370 375 380 Glu Phe Ala Glu Asn Gly Ser Leu His Asn Trp Leu Phe Ser Asn Ser 385 390 395 400 Ser Thr Gly Ser Arg Phe Leu Thr Trp Ser Gln Arg Ile Ser Ile Ala 405 410 415 Val Asp Val Ala Met Gly Leu Gln Tyr Met His Glu His Thr Gln Pro 420 425 430 Ser Ile Val His Arg Asp Ile Thr Ser Ser Asn Ile Leu Leu Asp Ser 435 440 445 Asn Phe Lys Ala Lys Ile Ala Asn Phe Ser Val Ala Arg Thr Ser Ile 450 455 460 Asn Pro Met Ile Leu Lys Val Asp Val Phe Gly Tyr Gly Val Val Leu 465 470 475 480 Leu Glu Leu Leu Ser Gly Lys Lys Ser Leu Thr Asn Asn Glu Ile Asn 485 490 495 His Ile Arg Glu Ile Phe Asp Leu Lys Glu Lys Arg Glu Glu Arg Ile 500 505 510 Arg Arg Trp Met Asp Pro Lys Ile Glu Ser Leu Tyr Pro Ile Asp Asp 515 520 525 Ala Leu Ser Leu Ala Phe Leu Ala Met Asn Cys Thr Ser Glu Lys Pro 530 535 540 Leu Ser Arg Pro Thr Met Gly Glu Val Val Leu Ser Leu Ser Leu Leu 545 550 555 560 Met Thr Gln His Ser Pro Thr Thr Leu Glu Arg Ser Trp Thr Cys Gly 565 570 575 Leu Asp Val Asp Val Thr Glu Met Gln Thr Leu Ile Ala Ala Arg 580 585 590 12 590 PRT 蒺藜苜蓿 12 Met Val Ser Ser Phe Phe His Thr Leu Ile Phe Phe Ser Ala Thr His 1 5 10 15 Ile Leu Leu Gln Leu Pro Gln Ala Asn Gly Lys Asn Phe Ser Cys Thr 20 25 30 Leu Asn Ser Ser Pro Ser Cys Asp Thr Tyr Val Ala Tyr Phe Ala Asn 35 40 45 Ser Pro Asn Phe Leu Thr Leu Thr Ala Ile Ser Asp Ile Phe Asp Thr 50 55 60 Ser Pro Gln Ser Ile Ala Arg Ala Ser Asn Ile Lys Asp Glu Asn Met 65 70 75 80 Asn Leu Ile His Gly Gln Leu Leu Leu Ile Pro Ile Thr Cys Gly Cys 85 90 95 Asn Gly Asn Gly Asn Tyr Ser Phe Ala Asn Ile Ser His Leu Ile Lys 100 105 110 Glu Ser Glu Ser Tyr Tyr Tyr Leu Ser Thr Ile Ser Tyr Gln Asn Leu 115 120 125 Thr Asn Trp Gln Thr Val Glu Asp Ser Asn Pro Asn Leu Asn Pro Tyr 130 135 140 Leu Leu Lys Ile Gly Thr Lys Ile Asn Ile Pro Leu Phe Cys Arg Cys 145 150 155 160 Pro Ser Asn Tyr Phe Ala Lys Gly Ile Glu Tyr Leu Ile Thr Tyr Val 165 170 175 Trp Gln Pro Asn Asp Asn Leu Thr Leu Val Ala Ser Lys Leu Gly Ala 180 185 190 Ser Pro Lys Asp Ile Ile Thr Ala Asn Thr Asn Asn Phe Gly Gln Asn 195 200 205 Phe Thr Val Ala Ile Asn Leu Pro Val Phe Ile Pro Val Lys Asn Leu 210 215 220 Pro Ala Leu Ser Gln Ser Tyr Tyr Ser Ser Ser Glu Arg Lys Arg Ile 225 230 235 240 Asn His Phe Ser Ile Ile Ile Ser Ile Gly Ile Cys Leu Gly Cys Thr 245 250 255 Ile Leu Ile Ser Leu Leu Leu Leu Leu Phe Tyr Val Tyr Cys Leu Arg 260 265 270 Lys Arg Lys Ala Cys Glu Asn Lys Cys Val Pro Ser Val Glu Ile Thr 275 280 285 Asp Lys Leu Ile Ser Glu Val Ser Asn Tyr Val Ser Lys Pro Thr Val 290 295 300 Tyr Glu Val Gly Met Ile Met Lys Ala Thr Met Asn Leu Asn Glu Met 305 310 315 320 Cys Lys Ile Gly Lys Ser Val Tyr Lys Ala Lys Ile Asp Gly Leu Val 325 330 335 Leu Ala Val Lys Asn Val Lys Gly His Ile Thr Val Thr Glu Glu Leu 340 345 350 Met Ile Leu Gln Lys Val Asn His Ala Asn Leu Val Lys Leu Val Gly 355 360 365 Val Ser Ser Gly Tyr Asp Gly Asn His Phe Leu Val Tyr Glu Tyr Ala 370 375 380 Glu Asn Gly Ser Leu Tyr Asn Trp Leu Leu Ser Glu Phe Cys Thr Leu 385 390 395 400 Ser Trp Ser Gln Arg Leu Ser Ile Ala Val Asp Ile Ala Ile Gly Leu 405 410 415 Gln Tyr Leu His Glu His Thr Gln Pro Cys Ile Val His Arg Asn Ile 420 425 430 Lys Ser Ser Asn Ile Leu Leu Asp Ser Lys Phe Lys Ala Lys Ile Ala 435 440 445 Asn Phe Ser Val Ala Arg Thr Thr Lys Asn Pro Met Ile Thr Lys Val 450 455 460 Asp Val Leu Gly Tyr Gly Met Val Leu Met Glu Leu Ile Thr Gly Lys 465 470 475 480 Lys Phe Leu Ser Tyr Ser Glu His Ser Glu Val Asn Met Leu Trp Lys 485 490 495 Asp Phe Lys Cys Val Phe Asp Thr Glu Gln Lys Arg Glu Glu Ile Val 500 505 510 Arg Arg Trp Met Asp Pro Lys Leu Gly Arg Phe Tyr Asn Val Val Glu 515 520 525 Ala Leu Ser Leu Phe Thr Leu Ala Val Asn Cys Ile Glu Glu Gln Pro 530 535 540 Leu Leu Arg Pro Thr Met Gly Glu Val Val Leu Ser Leu Ser Leu Leu 545 550 555 560 Thr Gln Pro Ser Pro Thr Leu Leu Glu Val Ser Trp Thr Tyr Gly Leu 565 570 575 Asp Val Glu Val Ala Glu Met Val Thr Pro Ile Ile Ala Arg 580 585 590 13 613 PRT Parasponia andersonii 13 Met Ala Ile Ser Leu Tyr Leu Leu Leu Phe Phe Ile Thr His Ile Ser 1 5 10 15 Ala Gln Ser Pro Pro Thr Leu Ala Thr Asn Phe Ser Cys Ser Thr Asn 20 25 30 Ser Ser Gln Pro Ser Cys Lys Thr Tyr Val Ala Tyr Phe Ala Gln Pro 35 40 45 Pro Leu Phe Met Asp Leu Lys Ser Ile Ser Asn Leu Phe Gly Val Ser 50 55 60 Pro Ser Ser Ile Ser Glu Ala Ser Asn Leu Val Ser Glu Ser Thr Lys 65 70 75 80 Leu Thr Arg Gly Gln Leu Leu Leu Ile Pro Leu Ser Cys Ser Cys Asn 85 90 95 Gly Ser His Tyr Phe Ser Asn Val Thr Tyr Asn Ile Thr Met Gly Asp 100 105 110 Ser Tyr Tyr Leu Val Ser Ile His Ser Phe Glu Asn Leu Thr Asn Trp 115 120 125 Pro Leu Val Arg Asp Thr Asn Pro Thr Leu Asn Pro Asn Leu Leu Gln 130 135 140 Ile Gly Thr Lys Val Ile Phe Pro Leu Tyr Cys Gly Cys Pro Ser Lys 145 150 155 160 Ser His Ser Lys Asn Gly Ile Lys Tyr Leu Ile Thr Tyr Val Trp Gln 165 170 175 Pro Ser Asp Asp Ile Tyr Arg Val Ser Ala Met Phe Asn Ala Ser Glu 180 185 190 Val Asp Ile Ile Ile Glu Asn Asn Tyr Gln Asp Phe Lys Ala Ala Val 195 200 205 Gly Tyr Pro Val Leu Ile Pro Val Ser Arg Met Pro Ala Leu Ser Gln 210 215 220 Pro Pro Tyr Pro Ser His Ser His His Arg Ser Gln Leu Lys His Arg 225 230 235 240 Trp Phe Leu Ile Ala Val Ile Ser Ser Ala Gly Ala Leu Leu Ile Leu 245 250 255 Phe Leu Ala Thr Phe Leu Val His Ser Ile Gly Leu Tyr Glu Lys Lys 260 265 270 Lys Asn Leu Ser His Glu Glu Ser Ser Leu Glu Thr Thr Asp Leu Ile 275 280 285 Gln Val Lys Asn Phe Ser Lys Ser Asp Thr Leu Glu Leu Gln Ala Lys 290 295 300 His Asp Lys Leu Leu Pro Gly Val Ser Val Tyr Leu Gly Lys Pro Ile 305 310 315 320 Met Tyr Glu Ile Lys Met Ile Met Glu Ala Thr Met Asn Phe Asn Asp 325 330 335 Gln Tyr Lys Ile Gly Gly Ser Val Tyr Arg Ala Met Ile Asn Gly Ser 340 345 350 Phe Leu Ala Val Lys Lys Ala Lys Glu Asn Val Thr Glu Glu Leu His 355 360 365 Ile Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Ile 370 375 380 Ser Leu Asp Arg Asp Gly Asn Cys Phe Phe Val Tyr Glu Tyr Ala Glu 385 390 395 400 Asn Gly Ser Leu Asp Lys Trp Leu Asn Pro Gln Ser Ser Thr Ser Thr 405 410 415 Ser Ser Ser Val Gly Ile Leu Ser Trp Ser Gln Arg Leu Asn Ile Ala 420 425 430 Leu Asp Val Ala Asn Gly Leu Gln Tyr Met His Glu His Thr Gln Pro 435 440 445 Ser Ile Val His Lys Glu Ile Arg Thr Ser Asn Ile Leu Leu Asp Ser 450 455 460 Arg Phe Lys Ala Lys Ile Ala Asn Phe Ser Met Ala Arg Ser Ala Ala 465 470 475 480 Ser Ala Gly Met Thr Lys Val Asp Val Phe Ala Phe Gly Val Val Leu 485 490 495 Leu Lys Leu Leu Ser Gly Arg Lys Ala Met Ala Thr Arg Glu Asn Gly 500 505 510 Glu Ile Val Met Leu Trp Lys Glu Ala Lys Ala Val Leu Glu Glu Glu 515 520 525 Glu Lys Arg Ala Glu Lys Val Arg Glu Trp Ile Asp Pro Lys Leu Glu 530 535 540 Ser Phe Tyr Pro Ile Asp Gly Ala Leu Ser Leu Met Thr Leu Ala Lys 545 550 555 560 Ala Cys Thr Gln Glu Lys Ala Ser Ala Arg Pro Ser Ile Gly Glu Val 565 570 575 Val Phe Ser Leu Cys Val Leu Thr Gln Ser Phe Ser Glu Thr Leu Glu 580 585 590 Pro Ser Trp Thr Cys Thr Leu Glu Gly Glu Asp Val Val Gln Ile Thr 595 600 605 Ser Pro Ile Val Ala 610 14 582 PRT Parasponia andersonii 14 Met Ala Asp Ser Tyr Phe Pro Phe Gln Ala Ile Phe Leu Leu Leu Leu 1 5 10 15 Leu Phe Ser Thr Leu Asn Met Ala Ala Ser Gln Leu Asn Asn Ser Ala 20 25 30 Thr Asp Phe Ser Cys Ser Asp Ser Pro Pro Ser Cys Glu Ala Tyr Val 35 40 45 Ala Tyr Phe Ser Gln Pro Pro Asn Tyr Met Asn Val Gly Asn Ile Ser 50 55 60 Asp Leu Phe Gly Ile Ser Gln Ala Leu Ile Ala Lys Ser Ser Asn Leu 65 70 75 80 Val Ser Lys Asp Ser Pro Leu Ile Pro Gln Gln Leu Leu Leu Ile Pro 85 90 95 Leu Thr Cys Thr Cys Thr Gly Asn His Tyr Phe Ala Asn Ile Thr Tyr 100 105 110 Gln Val Glu Pro Gly Asp Thr Tyr His Tyr Leu Ser Thr Leu Leu Phe 115 120 125 Glu Asn Leu Thr Asn Ser Gln Val Met Lys Lys Met Asn Pro Glu Ile 130 135 140 Ser Pro Glu Tyr Val Leu Pro Tyr Ile Asp Ile Ile Ile Pro Val Phe 145 150 155 160 Cys Arg Cys Pro Ser Lys Ser His Leu Lys Ser Glu Ile Gln Gln Phe 165 170 175 Ile Thr Tyr Val Trp Gln Pro Asn Asp Gln Val Ser Asn Val Ser Ala 180 185 190 Lys Phe Asn Thr Ser Ala Ser Glu Ile Val Asn Glu Asn Lys Tyr Asn 195 200 205 Asn Phe Ser Ser Ala Val Gly Leu Pro Val Leu Ile Pro Val Ser Lys 210 215 220 Leu Pro Val Leu Ala Arg Val Lys Pro Pro Lys Ser Val Arg Ser Lys 225 230 235 240 Lys Gln Trp Ile Leu Ile Gly Val Glu Ser Leu Gly Gly Ile Val Leu 245 250 255 Ile Thr Leu Phe Ala Thr Leu Leu Val Tyr Ser Asn Arg Leu Leu Lys 260 265 270 Lys Arg Arg Lys Ile Leu Glu Ala Arg Arg Leu Glu Pro Arg Ile Ile 275 280 285 Ile Gln Asp Lys Leu Leu Ser Gly Val Ser Glu Tyr Leu Gly Arg Pro 290 295 300 Ile Met Tyr Asp Asn Lys Met Val Val Glu Gly Thr Met Asp Phe Ser 305 310 315 320 Glu Gln Cys Arg Ile Gly Gly Ser Val Tyr Arg Gly Glu Ile Tyr Gly 325 330 335 Glu Val Phe Ala Val Lys Lys Thr Lys Gln Asp Ile Thr Asp Glu Leu 340 345 350 Asn Leu Leu Gln Lys Val Asn His Val Asn Leu Val Asn Leu Met Gly 355 360 365 Ala Ser Tyr Asp Thr Asp Gly Asn Arg Phe Leu Val Tyr Glu Tyr Val 370 375 380 Glu Asn Gly Ser Leu Glu Arg Trp Leu Asp Leu Lys Pro Ser Ser Leu 385 390 395 400 Ala Ala Ala Ser Ser Ser Ser Ser Val Gln Phe Leu Ser Trp Ser Gln 405 410 415 Arg Ile Gln Ile Ala Leu Asp Val Ala Asn Gly Leu Gln Tyr Leu His 420 425 430 Glu His Thr Gln Pro Asn Ile Ala His Trp Asn Ile Arg Thr Ser Thr 435 440 445 Ile Leu Leu Asp Ser Lys Phe Arg Ala Lys Ile Ala Asn Phe Glu Val 450 455 460 Ala Arg Pro Val Gly Asn Pro Ala Met Leu Lys Val Asp Ile Phe Ala 465 470 475 480 Phe Gly Ile Val Leu Leu Ala Leu Val Ser Gly Lys Lys Ala Leu Gln 485 490 495 Thr Ile Glu Asn Gly Glu Val Ile Met Leu Trp Lys Asp Leu Ala Lys 500 505 510 Glu Val Phe Glu Val Glu Glu Lys Lys Glu Asp Arg Leu Arg Lys Trp 515 520 525 Met Asp Pro Asn Leu Gln Ser Phe Tyr Pro Ile Asp Gly Ala Leu Ser 530 535 540 Leu Ser Ser Leu Ala Arg Ala Cys Ile Arg Glu Lys Ser Ser Ala Arg 545 550 555 560 Pro Lys Met Ala Glu Ile Val Phe Ser Leu Ser Val Leu Ala Gln Ser 565 570 575 Ser Ser Pro Gly Thr Pro 580 15 15 000 16 670 PRT 大麦 16 Met Ala Ala Pro Pro Gly Arg Arg Gly Leu Ala Phe Gly Thr Ala Ala 1 5 10 15 Leu Ala Leu Leu Ala Ile Leu Ala Val Ala Arg Gly Gln Gln Gln Tyr 20 25 30 Glu Ala Asn Ala Gln Thr Asn Cys Tyr Gly Arg Asn Gly Ser Ser Val 35 40 45 Leu Gly Tyr Val Cys Asn Ala Thr Ala Ala Ala Ala Pro Cys Ala Thr 50 55 60 Tyr Val Val Phe Arg Ser Ser Pro Pro Tyr Tyr Gly Thr Ala Val Ser 65 70 75 80 Ile Ser Tyr Leu Leu Gly Ser Asp Pro Glu Ala Val Ala Asp Ala Asn 85 90 95 Gly Val Pro Thr Val Ser Pro Leu Ala Asp Ser Arg Leu Val Leu Ala 100 105 110 Pro Val Pro Cys Gly Cys Ser Pro Arg Gly Tyr Tyr Gln His Asn Ser 115 120 125 Ser His Thr Ile Glu Leu Arg Gly Glu Thr Tyr Phe Ile Ile Ala Asn 130 135 140 Asn Thr Tyr Gln Gly Leu Thr Thr Cys Gln Ala Leu Leu Ala Gln Asn 145 150 155 160 Pro Arg His Gly Ser Arg Asp Leu Val Ala Gly Asn Asn Leu Thr Val 165 170 175 Pro Ile Arg Cys Ala Cys Pro Thr Pro Ala Gln Ala Ala Ala Gly Val 180 185 190 Arg His Leu Leu Thr Tyr Leu Val Thr Trp Gly Asp Ser Val Ser Ala 195 200 205 Ile Ala Asp Arg Phe Arg Val Asp Ala Gln Ala Val Phe Gln Ala Asn 210 215 220 Asn Leu Thr Ala Arg Glu Ile Ile Phe Pro Phe Thr Thr Leu Leu Ile 225 230 235 240 Pro Leu Lys Ser Ala Pro Thr Pro Asp Met Leu Val Ser Pro Ala Pro 245 250 255 Pro Pro Ala Pro Ala Pro Pro Gln Ala Gln Gln Pro Pro Ala Ser Gly 260 265 270 Ser Gly Lys Trp Ile Ala Val Gly Val Gly Val Gly Val Gly Val Leu 275 280 285 Ala Leu Ala Ser Leu Ile Gly Leu Met Leu Leu Cys Val Arg Arg Arg 290 295 300 Arg Thr Arg Gln Gly Val Arg Glu Arg Gly Arg Leu Ser Lys Val Val 305 310 315 320 Leu Asp Val Pro Ser Ser Ala Asp Tyr Asn Ala Leu Ala Ser Gly Lys 325 330 335 His Ala Ser Ser Ala Thr Thr Thr Ser Ala Ser Ser Ser Ala Leu Val 340 345 350 Ser Ser Asp Ala Arg Ala Ala Val Glu Ser Leu Thr Val Tyr Lys Tyr 355 360 365 Ser Glu Leu Glu Lys Ala Thr Ala Gly Phe Ser Glu Asp Arg Arg Val 370 375 380 Lys Asn Ala Ser Val Tyr Arg Ala Glu Ile Asn Gly Asp Ala Ala Ala 385 390 395 400 Val Lys Arg Val Ala Gly Asp Val Ser Gly Glu Val Gly Ile Leu Lys 405 410 415 Arg Val Asn His Ser Ser Leu Val Arg Leu Ser Gly Leu Cys Val His 420 425 430 His Gly Glu Thr Tyr Leu Val Phe Glu Phe Ala Glu Asn Gly Ala Leu 435 440 445 Ser Asp Trp Leu His Gly Gly Gly Ala Thr Leu Val Trp Lys Gln Arg 450 455 460 Val Gln Ala Ala Phe Asp Val Ala Asp Gly Leu Asn Tyr Leu His His 465 470 475 480 Tyr Thr Asn Pro Pro Cys Val His Lys Asn Leu Lys Ser Ser Asn Val 485 490 495 Leu Leu Asp Ala Asn Leu Arg Ala Lys Val Ser Ser Phe Ala Leu Ala 500 505 510 Arg Ser Val Pro Thr Gly Ala Asp Gly Gly Asp Ala Gln Leu Thr Arg 515 520 525 His Val Val Gly Thr Gln Gly Tyr Leu Ala Pro Glu Tyr Leu Glu His 530 535 540 Gly Leu Ile Thr Pro Lys Leu Asp Val Phe Ala Phe Gly Val Ile Leu 545 550 555 560 Leu Glu Leu Leu Ser Gly Lys Glu Ala Met Phe Asn Gly Gly Asp Lys 565 570 575 Arg Gly Glu Thr Leu Leu Trp Glu Ser Ala Glu Gly Leu Val Val Asp 580 585 590 Asn Glu Asp Ala Arg Gly Lys Val Arg Pro Phe Met Asp Pro Arg Leu 595 600 605 His Gly Asp Tyr Pro Leu Asp Leu Ala Val Ala Val Ala Ser Leu Ala 610 615 620 Val Arg Cys Val Ala Arg Glu Pro Arg Arg Arg Pro Ser Ile Asp Val 625 630 635 640 Val Phe Ala Thr Leu Ser Ala Val Tyr Asn Ser Thr Leu Asp Trp Asp 645 650 655 Pro Ser Asp Asp Gly Asn Ser Arg Ser Ser Ile Val Gly Arg 660 665 670 17 650 PRT 大麦 17 Met Ala Pro Leu Thr Arg Arg Arg Arg Leu Leu Ala Thr Leu Leu Cys 1 5 10 15 Leu Cys Ala Leu Pro Ala Pro Ala Arg Ser Gln Asn Ala Ser Ala Thr 20 25 30 Pro Ala Pro Ala Ser Val Glu Gly Phe Asn Cys Ser Ala Asn Gly Thr 35 40 45 Tyr Pro Cys Gln Ala Tyr Ala Leu Tyr Arg Ala Gly Leu Ala Gly Val 50 55 60 Pro Pro Asp Leu Ser Ala Ala Gly Asp Leu Phe Gly Val Ser Arg Phe 65 70 75 80 Met Leu Ala His Ala Asn Asn Leu Ser Thr Ser Ala Ala Pro Ala Ala 85 90 95 Gly Gln Pro Leu Leu Val Pro Leu Gln Cys Gly Cys Pro Ser Gly Ser 100 105 110 Pro Asn Ala Tyr Ala Pro Thr Gln Tyr Gln Ile Ser Ser Gly Asp Thr 115 120 125 Phe Trp Ile Val Ser Val Thr Lys Leu Gln Asn Leu Thr Gln Tyr Gln 130 135 140 Ala Val Glu Arg Val Asn Pro Thr Val Val Pro Thr Lys Leu Glu Val 145 150 155 160 Gly Asp Met Val Thr Phe Pro Ile Phe Cys Gln Cys Pro Thr Ala Ala 165 170 175 Gln Asn Ala Thr Ala Leu Val Thr Tyr Val Met Gln Gln Gly Asp Thr 180 185 190 Tyr Ala Ser Ile Ala Ala Ala Phe Ala Val Asp Ala Gln Ser Leu Val 195 200 205 Ser Leu Asn Gly Pro Glu Gln Gly Thr Gln Leu Phe Ser Glu Ile Leu 210 215 220 Val Pro Leu Arg Arg Gln Val Pro Lys Trp Leu Pro Pro Ile Val Thr 225 230 235 240 Arg Asn Asp Ala Ser Ala Thr Pro Pro Ser Pro Ser Pro Pro Pro Thr 245 250 255 Thr Thr Pro Gly Pro Ser Asp Val Ala Asp Asn Arg Asp Gly Val Val 260 265 270 Thr Gly Leu Ala Val Gly Leu Gly Val Val Gly Gly Leu Trp Leu Leu 275 280 285 Gln Leu Leu Leu Leu Gly Cys Leu Trp Arg Arg Leu Lys Ala Lys Gly 290 295 300 Arg Arg Gly Asp Ala Val Ala Ser Gly Glu Gly Gly Glu Gly Gly Arg 305 310 315 320 Ser Ala Lys Thr Ala Ser Ala Ser Gly Gly Val Gly Gly Glu Arg Phe 325 330 335 Leu Val Thr Asp Ile Ser Glu Trp Leu Asp Lys Tyr Arg Val Phe Lys 340 345 350 Val Glu Glu Leu Glu Arg Gly Thr Asp Gly Phe Asp Asp Ala His Leu 355 360 365 Ile Gln Gly Ser Val Tyr Lys Ala Asn Ile Gly Gly Glu Val Phe Ala 370 375 380 Val Lys Lys Met Lys Trp Asp Ala Cys Glu Glu Leu Lys Ile Leu Gln 385 390 395 400 Lys Val Asn His Ser Asn Leu Val Lys Leu Glu Gly Phe Cys Ile Asn 405 410 415 Thr Ala Thr Gly Asp Cys Phe Leu Val Tyr Glu Tyr Val Glu Asn Gly 420 425 430 Ser Leu Asp Leu Cys Leu Leu Asp Arg Gly Arg Ala Arg Arg Leu Asp 435 440 445 Trp Arg Thr Arg Leu His Ile Ala Leu Asp Leu Ala His Gly Leu Gln 450 455 460 Tyr Ile His Glu His Thr Trp Pro Arg Val Val His Lys Asp Val Lys 465 470 475 480 Ser Ser Asn Val Leu Leu Asp Ala Arg Met Arg Ala Lys Ile Ala Asn 485 490 495 Phe Gly Leu Ala Lys Thr Gly His Asn Ala Val Thr Thr His Ile Val 500 505 510 Gly Thr Gln Gly Tyr Ile Ala Pro Glu Tyr Leu Val Asp Gly Leu Val 515 520 525 Thr Thr Lys Met Asp Val Phe Ala Tyr Gly Val Val Leu Leu Glu Leu 530 535 540 Val Ser Gly Arg Glu Ala Ala Gly Asp Gly Gly Asp Leu Leu Leu Ala 545 550 555 560 Asp Ala Glu Glu Arg Val Phe Arg Gly Arg Glu Asp Arg Leu Glu Ala 565 570 575 Arg Ala Ala Ala Trp Met Asp Pro Val Leu Ala Glu Gln Thr Cys Pro 580 585 590 Pro Gly Ser Val Ala Thr Val Met Gly Val Ala Arg Ala Cys Leu Gln 595 600 605 Arg Asp Pro Ser Lys Arg Pro Ser Met Val Asp Val Ala Tyr Thr Leu 610 615 620 Ser Arg Ala Asp Glu Tyr Phe Ala Asp Tyr Ser Gly Glu Ser Val Ser 625 630 635 640 Val Asp Gly Ser Gly Glu Ile Ala Ala Arg 645 650 18 681 PRT 大麦 18 Met Ala Thr Pro Thr Arg Trp Arg Gly Leu Ala Ala Val Gly Arg Ala 1 5 10 15 Ala Leu Ala Phe Leu Val Leu Leu Ala Val Ala Ala Pro Trp Cys Pro 20 25 30 Val Ala Arg Gly Gln Gln Glu Tyr Glu Ala Asn Ala Gln Asn Asn Cys 35 40 45 Tyr Gly Asn Asn Gly Ser Ser Val Leu Gly Tyr Thr Cys Asn Ala Thr 50 55 60 Ala Ala Val Arg Pro Cys Ala Ser Tyr Val Val Phe Arg Ser Ser Pro 65 70 75 80 Pro Tyr Glu Ser Pro Ile Thr Ile Ser Tyr Leu Leu Asn Thr Thr Pro 85 90 95 Ala Ala Leu Ala Asp Ala Asn Ala Val Pro Thr Val Ser Ser Val Ala 100 105 110 Ala Ser Arg Leu Val Leu Ala Pro Leu Asn Cys Gly Cys Ala Pro Gly 115 120 125 Gly Tyr Tyr Gln His Asn Ala Ser Tyr Thr Leu Gln Phe Ser Asn Glu 130 135 140 Thr Tyr Phe Ile Thr Ala Asn Ile Thr Tyr Gln Gly Leu Thr Thr Cys 145 150 155 160 Gln Ala Leu Met Ala Gln Asn Pro Asn His Asp Ser Arg Asn Leu Val 165 170 175 Val Gly Asn Asn Leu Thr Val Pro Ile Arg Cys Ala Cys Pro Ser Pro 180 185 190 Ala Gln Ala Ala Ser Gly Val Arg His Leu Leu Thr Tyr Leu Val Ala 195 200 205 Ser Gly Asp Thr Ile Ala Asp Ile Ala Thr Arg Phe Arg Val Asp Ala 210 215 220 Gln Ala Val Leu Arg Ala Asn Arg Leu Thr Asp Ser Glu Asn Ile Tyr 225 230 235 240 Pro Phe Thr Thr Leu Leu Ile Pro Leu Lys Ser Ala Pro Thr Pro Asp 245 250 255 Met Leu Val Ser Pro Ala Pro Pro Pro Ala Pro Val Pro Pro Gln Ala 260 265 270 Gln Gln Pro Leu Pro Thr Gly Gly Ser Gly Ser Gly Lys Gly Val Ala 275 280 285 Ile Gly Val Gly Val Gly Val Gly Val Leu Ala Leu Ala Gly Leu Leu 290 295 300 Gly Leu Met Phe Leu Cys Val Arg Arg Arg Arg Arg Leu Arg Pro Gly 305 310 315 320 Val Gly Glu Asn Gly His Pro Gly Lys Val Val Ile Asp Val Pro Ser 325 330 335 Ser Ala Asp Tyr Asp Pro Leu Ala Ser Gly Lys His Thr Ser Ser Ala 340 345 350 Thr Thr Thr Ser Ser Ser Ser Ser Ala Phe Val Ser Ser Asp Ala Arg 355 360 365 Ala Ala Val Glu Ser Leu Thr Val Tyr Lys Tyr Ser Glu Leu Glu Lys 370 375 380 Ala Thr Ala Gly Phe Ser Glu Asp Arg Arg Val Lys Asp Ala Ser Val 385 390 395 400 Tyr Arg Ala Val Ile Asn Gly Asp Thr Ala Ala Val Lys Arg Val Ala 405 410 415 Gly Asp Val Ser Gly Glu Val Gly Ile Leu Lys Arg Val Asn His Ser 420 425 430 Ser Leu Val Arg Leu Ser Gly Leu Cys Val His His Gly Asp Thr Tyr 435 440 445 Leu Val Phe Glu Phe Ala Glu Asn Gly Ala Leu Ser Asp Trp Leu His 450 455 460 Gly Gly Gly Ala Thr Leu Val Trp Lys Gln Arg Val Gln Ala Ala Phe 465 470 475 480 Asp Val Ala Asp Gly Leu Asn Tyr Leu His His Tyr Ser Thr Pro Pro 485 490 495 Cys Val His Lys Asn Leu Lys Ser Ser Asn Val Leu Leu Asp Ala Asp 500 505 510 Leu Arg Ala Lys Val Ser Ser Phe Ala Leu Ala Arg Ser Val Pro Thr 515 520 525 Gly Ala Glu Gly Gly Asp Ala Gln Leu Thr Arg His Val Val Gly Thr 530 535 540 Gln Gly Tyr Leu Ala Pro Glu Tyr Leu Glu His Gly Leu Ile Thr Pro 545 550 555 560 Lys Leu Asp Val Phe Ala Phe Gly Val Ile Leu Leu Glu Leu Leu Ser 565 570 575 Gly Lys Glu Ala Thr Phe Asn Gly Gly Asp Lys Arg Gly Glu Lys Leu 580 585 590 Leu Trp Glu Ser Ala Glu Gly Leu Val Val Asp Gly Glu Asp Ala Arg 595 600 605 Ser Lys Val Arg Ala Phe Met Asp Pro Gln Leu Ser Gly Asp Tyr Pro 610 615 620 Leu Asp Leu Ala Val Ala Val Ala Ser Leu Ala Leu Arg Cys Val Ala 625 630 635 640 Arg Glu Pro Arg Gly Arg Pro Ser Met Tyr Glu Val Phe Val Thr Leu 645 650 655 Ser Ala Val Tyr Asn Ser Thr Leu Asp Trp Asp Pro Ser Asp Tyr Ser 660 665 670 Asn Ser Arg Ser Ser Ile Val Gly Arg 675 680 19 633 PRT 大麦 19 Met Glu Pro Arg Arg Phe Leu Cys Cys Cys Leu Val Ala Val Leu Ala 1 5 10 15 Val Ala Ser Arg Arg Cys Asp Ala Gln Gly Gly Ala Gly Asn Gly Thr 20 25 30 Gly Arg Phe Ala Cys Val Val Pro Ala Pro Cys Asp Thr Phe Val Leu 35 40 45 Tyr Arg Thr Gln Ser Pro Gly Ser Leu Asp Leu Gly Ala Ile Ser Asp 50 55 60 Leu Phe Gly Val Ser Arg Ala Met Ile Ala Ala Ala Asn Asn Leu Ser 65 70 75 80 Leu Ile Asp Glu Asp Ala Ala Leu Leu Pro Asp Gln Pro Leu Leu Val 85 90 95 Pro Val Arg Cys Gly Cys Thr Gly Asn Arg Ser Phe Val Asn Val Thr 100 105 110 Tyr Pro Ile His Ser Gly Asp Thr Phe Tyr Ala Leu Ala Leu Thr Gly 115 120 125 Tyr Glu Asn Leu Thr Thr Pro Asp Val Ile Gln Glu Leu Asn Pro Gln 130 135 140 Ala Val Phe Asn Lys Leu Asn Val Ser Gln Leu Val Thr Val Pro Leu 145 150 155 160 Phe Cys Arg Cys Pro Thr Pro Ala Glu Arg Ser Ala Gly Val Leu Gln 165 170 175 Gln Ile Thr Tyr Met Trp Arg Pro Val Asp Thr Met Ser Arg Val Ser 180 185 190 Lys Leu Met Gly Ser Asp Ala Ser Ala Ile Ala Ala Ala Asn Asn Val 195 200 205 Ser Ala Asp Phe Thr Ser Thr Thr Met Leu Pro Met Leu Ile Pro Val 210 215 220 Ala Arg Pro Pro Val Leu Pro Pro Leu Arg Tyr Gly Pro Ser Ala Thr 225 230 235 240 Thr Gly Asp Pro Gly Ala Thr Lys Arg Phe Ser Gly Ala Thr Val Ala 245 250 255 Ala Ser Ile Ala Gly Ser Leu Val Ala Val Ala Ala Leu Cys Val Ala 260 265 270 Ile Phe Gly Tyr Arg Arg Tyr Arg Arg Lys Lys Ala Thr Val His Ser 275 280 285 Ala Ser Arg Phe Ala Ser Pro Arg Phe Cys Phe Asn Gln Asn Ala Tyr 290 295 300 Gly Ile Gln Ser Ser Ser Ser Ile Ala Arg Met Ile Asn Gly Gly Asp 305 310 315 320 Lys Leu Leu Thr Ser Val Ser Gln Phe Ile Asp Lys Pro Val Ile Phe 325 330 335 Gly Thr Ala Glu Ile Met Glu Ala Thr Met Asn Leu Asp Glu Arg Cys 340 345 350 Arg Ile Gly Ser Ser Tyr Tyr Arg Ala Lys Leu Glu Gly Glu Val Phe 355 360 365 Ala Val Lys Pro Ala Lys Gly Asp Val Ser Ala Glu Leu Arg Met Met 370 375 380 Gln Met Val Asn His Ala Asn Leu Ile Arg Leu Ala Gly Ile Ser Ile 385 390 395 400 Gly Ala Asp Gly Asp Tyr Thr Phe Leu Val Tyr Glu Phe Ala Glu Lys 405 410 415 Gly Ser Leu Asp Lys Trp Leu Tyr Gln Lys Pro Pro Ser Ser Leu Pro 420 425 430 Ser Ser Ser Ser Ser Val Asp Thr Leu Ser Trp Asn Gln Arg Leu Gly 435 440 445 Ile Ala Leu Asp Val Ala Asn Gly Leu Leu Tyr Met His Glu His Thr 450 455 460 Gln Pro Ser Met Val His Gly Asp Val Arg Ala Arg Asn Ile Leu Leu 465 470 475 480 Thr Ala Asp Phe Arg Ala Arg Ile Ser Asn Phe Ser Val Ala Thr Pro 485 490 495 Ala Met Ala Asp Ala Ala Ala Thr Ser Ser Asp Val Phe Ala Phe Gly 500 505 510 Leu Leu Val Leu Glu Leu Leu Ser Gly Arg Thr Ala Met Glu Ala Arg 515 520 525 Val Gly Ala Glu Ile Gly Met Leu Trp Arg Asp Ile Arg Ala Val Leu 530 535 540 Glu Ala Gly Asp Lys Arg Asp Ala Lys Leu Arg Lys Trp Met Asp Pro 545 550 555 560 Ala Leu Gly Asp Glu Tyr Tyr Leu Asp Ala Ala Leu Ser Leu Ala Gly 565 570 575 Met Ala Arg Ala Cys Thr Glu Glu Asp Ala Ala Arg Arg Pro Lys Met 580 585 590 Ala Asp Val Val Phe Ser Leu Ser Met Leu Val Gln Pro Leu Pro Val 595 600 605 Gly Asp Ala Phe Glu Lys Leu Trp Gln Pro Ser Ser Glu Glu Asn Ile 610 615 620 Arg Ile Val Asn Glu Val Ala Ala Arg 625 630 20 638 PRT 玉米 20 Met Glu Pro Arg His Phe Cys Arg Ala Leu Leu Leu Leu Leu Val Val 1 5 10 15 Leu Leu Leu Gly Phe Arg Arg Ala Gly Ala Gln Asp Ser Thr Asn Tyr 20 25 30 Thr Val Pro Ala Arg Phe Ala Cys Asn Val Ser Ser Pro Cys Asp Thr 35 40 45 Tyr Val Val Tyr Arg Thr Gln Ser Pro Gly Tyr Leu Asp Leu Gly Ser 50 55 60 Ile Ser Asp Leu Phe Gly Thr Ser Gln Ala Arg Ile Ala Ser Ala Asn 65 70 75 80 Gly Leu Ser Ser Glu Asp Gly Val Leu Gln Pro Gly Gln Pro Leu Leu 85 90 95 Val Pro Val Arg Cys Gly Cys Thr Gly Ala Trp Ser Phe Ala Asn Ala 100 105 110 Thr Tyr Pro Ile Arg Gln Gly Asp Thr Phe Tyr Asn Leu Ala Arg Leu 115 120 125 Ser Tyr Glu Asn Leu Thr Glu Tyr His Leu Ile His Asp Leu Asn Pro 130 135 140 Arg Ser Glu Pro Thr Ser Leu Gln Ile Gly Gln Glu Val Thr Val Pro 145 150 155 160 Leu Leu Cys Arg Cys Pro Pro Ala Arg Ala Val Gln Ser Phe Ile Thr 165 170 175 Tyr Val Trp Gln Pro Gly Asp Thr Leu Ser Gln Val Ser Lys Leu Met 180 185 190 Asn Ala Thr Ala Asp Glu Ile Ala Glu Ala Asn Asn Val Thr Ser Ser 195 200 205 Ser Val Ser Ser Ala Ser Ala Ala Gly Leu Pro Met Leu Ile Pro Val 210 215 220 Gln Gln Arg Pro Arg Leu Pro Pro Leu Leu Tyr Ala Ala Ser Ala Gly 225 230 235 240 Glu Gly Arg Ser Ser Arg Ser Arg Arg Arg Ala Leu Ile Ile Ile Gly 245 250 255 Ala Ser Val Ser Gly Ser Leu Val Ala Leu Ala Ala Leu Leu Val Ala 260 265 270 Ile Met Ala Gln Arg Arg Tyr Arg Arg Lys Lys Pro Ser Met Arg Leu 275 280 285 Gly Ser Pro Phe Ala Val Asn Thr Lys Leu Ser Trp Ser Val Asn Gln 290 295 300 Tyr Gly His Gly Ser Ser Asn Ser Phe Ala His Val Met Lys Gly Gly 305 310 315 320 Lys Leu Leu Thr Gly Val Ser Gln Phe Ile Asp Lys Pro Ile Ile Phe 325 330 335 Val Glu Glu Glu Ile Val Glu Ala Thr Met Asn Leu Asp Glu Arg Cys 340 345 350 Lys Ile Gly Ser Thr Tyr Tyr Arg Ala Lys Leu Asp Gly Glu Val Phe 355 360 365 Ala Val Lys Pro Ala Lys Gly Asp Val Ser Ala Glu Leu Arg Met Met 370 375 380 Gln Met Val Asn His Ala Asn Leu Ile Lys Leu Ala Gly Ile Ser Ile 385 390 395 400 Gly Ala Asp Gly Asp Tyr Ala Phe Leu Val Tyr Glu Phe Ala Glu Lys 405 410 415 Ala Ser Leu Asp Lys Trp Leu Tyr His Asn His Gln Lys Pro Pro Ser 420 425 430 Ala Leu Leu Pro Ser Ser Ser Cys Thr Val Pro Thr Thr Leu Ser Trp 435 440 445 Gly Gln Arg Leu Ser Ile Ala Leu Asp Val Ala Asn Gly Leu Leu Tyr 450 455 460 Met His Glu His Thr Gln Pro Ser Met Val His Gly Asp Ile Arg Ala 465 470 475 480 Arg Asn Ile Leu Leu Thr Ala Asp Phe Arg Ala Lys Ile Ser Ser Phe 485 490 495 Ser Leu Ala Lys Pro Ala Thr Ala Asp Ala Ala Ala Thr Ser Ser Asp 500 505 510 Val Phe Ala Phe Gly Leu Leu Leu Leu Glu Leu Met Ser Gly Arg Arg 515 520 525 Ala Met Glu Ala Arg Ile Gly Ser Glu Ile Gly Met Leu Trp Arg Glu 530 535 540 Ile Arg Ala Val Leu Glu Ala Gly Asp Lys Arg Glu Ala Lys Leu Arg 545 550 555 560 Lys Trp Met Asp Pro Ala Leu Gly Ser Glu Tyr Gln Met Asp Ala Ala 565 570 575 Leu Ser Leu Ala Gly Met Ala Arg Ala Cys Thr Asp Glu Asp Ala Ala 580 585 590 Arg Arg Pro Asn Met Thr Glu Val Val Phe Ser Leu Ser Met Leu Ala 595 600 605 Gln Pro Leu Ser Val Ala Asp Gly Phe Glu Lys Leu Trp Gln Pro Ser 610 615 620 Ser Glu Asp Asn Ile Arg Ile Ala Gly Ser Val Ala Ala Arg 625 630 635 21 636 PRT 玉米 21 Met Glu Leu Arg His Phe Arg Cys Cys Ala Ser Arg Leu Leu Leu Leu 1 5 10 15 Val Thr Leu Leu Leu Gly Phe Arg Arg Ala Gly Ala Gln Asp Ser Thr 20 25 30 Ser Tyr Thr Val Pro Ala Gln Phe Ala Cys Asp Val Ser Ser Pro Cys 35 40 45 Asp Thr Tyr Val Val Tyr Arg Thr Gln Ser Pro Gly Tyr Leu Asp Leu 50 55 60 Gly Ser Ile Ser Asp Leu Phe Gly Thr Ser Gln Ala Arg Ile Ala Ser 65 70 75 80 Ala Asn Gly Leu Ser Ser Glu Asp Gly Val Leu Gln Pro Gly Gln Pro 85 90 95 Leu Leu Val Pro Val Arg Cys Gly Cys Ala Gly Ala Trp Ser Phe Ala 100 105 110 Asn Val Thr Tyr Pro Ile Arg Gln Gly Asp Thr Phe Tyr Asn Leu Ala 115 120 125 Lys Ala Ser Tyr Glu Asn Leu Thr Glu Tyr His Leu Ile Gln Asn Leu 130 135 140 Asn Pro Gly Ser Glu Pro Thr Ser Leu Gln Ile Gly Gln Glu Val Thr 145 150 155 160 Val Pro Leu Leu Cys Arg Cys Pro Ala Arg Ala Glu Arg Ser Arg Gly 165 170 175 Val Gln Ser Leu Ile Thr Tyr Met Trp Gln Ala Gly Asp Thr Met Ser 180 185 190 Gln Val Ser Lys Leu Met Asn Ala Thr Val Asp Glu Ile Ala Glu Ala 195 200 205 Asn Asn Val Thr Ala Asn Thr Ser Ala Ser Ala Ser Phe Val Gly Gln 210 215 220 Pro Met Leu Ile Pro Val Arg Gln Arg Pro Arg Leu Pro Ala Pro Leu 225 230 235 240 Tyr Ala Ala Ala Ala Ala Asp Gly Lys Ser Arg Ser Arg Arg Arg Ala 245 250 255 Ala Val Ile Gly Ala Ser Val Ser Gly Ser Leu Val Ala Leu Ala Ala 260 265 270 Leu Phe Val Ala Ile Leu Ala Arg Arg Arg Tyr Arg Lys Lys Pro Ser 275 280 285 Met Arg Leu Gly Ser Arg Phe Ala Val Asn Thr Lys Leu Ser Trp Ser 290 295 300 Arg Asn Gln Phe Gly His Asp Gly Ser Asn Ser Phe Ala His Val Met 305 310 315 320 Lys Gly Gly Lys Leu Leu Thr Gly Val Ser Gln Phe Ile Asp Lys Pro 325 330 335 Ile Ile Phe Val Glu Glu Glu Ile Met Glu Ala Thr Met Asn Leu Asp 340 345 350 Glu Arg Cys Lys Ile Gly Ser Thr Tyr Tyr Arg Ala Lys Leu Asp Gly 355 360 365 Glu Val Phe Ala Val Lys Pro Ala Lys Gly Asp Val Ser Ala Glu Leu 370 375 380 Lys Met Met Gln Met Val Asn His Ala Asn Leu Ile Lys Leu Ala Gly 385 390 395 400 Ile Ser Ile Gly Ala Asp Gly Asp Tyr Ala Phe Leu Val Tyr Glu Phe 405 410 415 Ala Glu Lys Gly Ser Leu Asp Lys Trp Leu Tyr Glu Lys Pro Pro Ser 420 425 430 Ala Leu Pro Ser Ser Ser Cys Thr Val Ala Thr Leu Ser Trp Gly Gln 435 440 445 Arg Leu Ser Ile Ala Leu Asp Val Ala Asn Gly Leu Leu Tyr Met His 450 455 460 Glu His Thr Gln Pro Ser Met Val His Asp Asp Ile Arg Ala Arg Asn 465 470 475 480 Ile Leu Leu Thr Ala Asp Phe Arg Ala Lys Ile Ser Gly Phe Ser Leu 485 490 495 Ala Lys Pro Ala Met Val Asp Ala Ala Ala Thr Ser Ser Asp Val Phe 500 505 510 Ala Phe Gly Leu Leu Leu Leu Glu Leu Leu Ser Gly Arg Arg Ala Met 515 520 525 Glu Ala Arg Ile Gly Ser Glu Ile Gly Met Leu Trp Arg Glu Ile Arg 530 535 540 Gly Val Leu Glu Thr Gly Asp Lys Arg Glu Ala Lys Leu Arg Lys Trp 545 550 555 560 Met Asp Pro Ala Leu Gly Ser Glu Tyr His Met Asp Val Ala Leu Ser 565 570 575 Leu Ala Ser Met Ala Arg Ala Cys Thr Glu Glu Asp Ala Ala Arg Arg 580 585 590 Pro Asn Met Thr Glu Val Val Phe Ser Leu Ser Val Leu Ala Gln Pro 595 600 605 Leu Ser Val Ala Asp Gly Phe Glu Lys Leu Trp Gln Pro Ser Ser Glu 610 615 620 Asp Asn Ile Arg Ile Ala Gly Ser Val Ala Ala Arg 625 630 635 22 620 PRT 苹果 22 Met Ala Ile Ser Phe Leu Cys Ser Lys Pro Leu Cys Ile Leu Leu Leu 1 5 10 15 Leu Leu Phe Phe Thr Ala Arg Ile Leu Ala Gln Ser Thr Pro Ser Asn 20 25 30 Ser Ser Thr Ser Phe Ser Cys Ser Val Asp Ala Pro Pro Ser Cys Asp 35 40 45 Thr Tyr Val Ser Tyr Phe Ala Arg Pro Gln Phe Met Ser Leu Glu Asn 50 55 60 Ile Ser His Leu Phe Gly Val Ser Pro Leu Ser Ile Ala Lys Ala Ser 65 70 75 80 Asn Leu Val Ser Glu His Ile Arg Leu Ile Ala Gly Gln Leu Leu Leu 85 90 95 Val Pro Ile Ser Cys Gly Cys Ser Gly Asn Ser Tyr Phe Ser Asn Ile 100 105 110 Thr Tyr Glu Ile Lys Ser Gly Asp Ser Phe Tyr Leu Val Ser Ile Asn 115 120 125 Ser Phe Glu Asn Leu Thr Asp Trp His Glu Val Leu Asn Met Asn Pro 130 135 140 Thr Leu Asp Pro Ser Leu Leu Gln Ile Gly Gln Lys Val Ile Phe Pro 145 150 155 160 Leu Phe Cys Lys Cys Pro Ser Lys Met Tyr Thr Glu Asn Gly Ile Lys 165 170 175 Tyr His Ile Thr Tyr Ile Trp Gln Pro Asn Asp Asp Ile Ser Arg Val 180 185 190 Ser Ser Arg Phe Asn Val Ser Thr Leu Asp Ile Ser Ser Ala Asn Asn 195 200 205 Leu His Asn Asp Ser Ala Ala Val Glu Leu Pro Val Val Ile Pro Val 210 215 220 Ser Arg Leu Pro Ala Leu Val Gln Pro Lys Pro Pro Gln Gly Arg Asn 225 230 235 240 Ile Phe Lys Gln Arg Trp Trp Leu Ile Leu Ile Ile Ile Leu Gly Gly 245 250 255 Val Leu Leu Val Ser Ser Leu Leu Ala Ile Phe Ala Val Tyr Thr Arg 260 265 270 His Gln His Lys Val Lys Lys Ala Leu Asp Gly Pro Gly Ser Ser Leu 275 280 285 Glu Ser Ala Glu Trp Phe Lys Met Lys Glu Gly Lys Ile Asp Glu Asn 290 295 300 Phe Asp Leu Lys Phe Ile Gln Asp Lys Leu Leu Pro Gly Val Ser Ser 305 310 315 320 Tyr Leu Gly Lys Pro Ile Met Tyr Glu Val Lys Thr Ile Met Glu Ala 325 330 335 Thr Met Asn Leu Asn Glu His Cys Arg Ile Gly Gly Ser Val Tyr Arg 340 345 350 Ala Ile Val Asp Gly Gln Val Leu Ala Val Lys Asn Thr Lys Glu Asp 355 360 365 Val Thr Glu Glu Leu Asn Ile Leu Gln Lys Val Asn His Ala Asn Leu 370 375 380 Val Lys Leu Met Gly Val Ser Ser Glu Thr Asp Gly Ser Arg Phe Leu 385 390 395 400 Val Tyr Glu Tyr Ala Ala Asn Gly Ser Leu Asp Lys Trp Leu Tyr Ser 405 410 415 Lys Ser Ser Ala Thr Ser Ser Ser Ala Glu Leu Leu Thr Trp Asn Gln 420 425 430 Arg Leu Ser Ile Ala Leu Asp Ile Ala Asn Gly Leu Gln Tyr Met His 435 440 445 Glu His Thr Gln Arg Ser Ile Val His Met Asp Ile Arg Thr Ser Asn 450 455 460 Ile Leu Leu Asp Ser Lys Phe Lys Ala Lys Ile Ala Asn Phe Ser Met 465 470 475 480 Ala Arg Ala Ala Ala Asn Asp Val Thr Pro Lys Val Asp Val Phe Ala 485 490 495 Phe Gly Val Val Leu Leu Ala Leu Leu Ser Gly Lys Lys Gly Met Glu 500 505 510 Ala Lys Glu Asn Gly Glu Ala Ile Met Leu Trp Lys Asp Val Arg Trp 515 520 525 Val Leu Glu Ala Glu Glu Glu Lys Val Glu Arg Leu Arg Lys Trp Met 530 535 540 Asp Pro Asn Leu Glu Asn Phe Tyr Pro Ile Asp Gly Ala Leu Ser Leu 545 550 555 560 Thr Ala Leu Ala Arg Ala Cys Thr Gln Glu Lys Pro Ser Thr Arg Pro 565 570 575 Ser Met Gly Glu Val Val Phe Asn Leu Ser Val Leu Thr His Ser Ser 580 585 590 Ser Gln Ser Thr Leu Glu Arg Ser Trp Thr Ser Ala Leu Glu Ala Glu 595 600 605 Glu Val Leu Glu Thr Ile Ser Pro Ile Ala Ala Arg 610 615 620 23 624 PRT 野草莓亚种草莓 23 Met Ala Val Ser Phe Leu Cys Ser Ser Met Val Cys Ile Leu Leu Leu 1 5 10 15 Phe Phe Phe Thr Ser Gln Ile Leu Ala Gln Pro Ala Pro Gln Ser Asn 20 25 30 Ser Thr Thr Ser Phe Ser Cys Ala Val Asp Ala Pro Ser Ser Cys Glu 35 40 45 Thr Tyr Val Ala Tyr Phe Val Glu Ser Pro Gly Tyr Met Asn Leu Glu 50 55 60 Asn Ile Ser Asp Leu Phe Gly Val Ser Val Ser Ser Ile Ser Gln Ala 65 70 75 80 Ser Asn Leu Ala Ser Ser Tyr Thr Gly Gln Thr Arg Leu Val Ala Gly 85 90 95 Gln Leu Leu Leu Val Pro Ile Thr Cys Gly Cys Thr Gly Asn Arg Ser 100 105 110 Phe Ala Asn Ile Thr Tyr Ser Ile Lys Arg Gly Asp Ser Tyr Tyr Val 115 120 125 Val Ser Met Tyr Thr Phe Glu Asn Leu Thr Arg Trp Pro Leu Val Val 130 135 140 Glu Met Asn Pro Ala Leu Val Pro Ser Leu Leu Gln Ile Gly Val Lys 145 150 155 160 Val Ile Phe Pro Leu Phe Cys Lys Cys Pro Ser Lys Met Tyr Ser Asp 165 170 175 Leu Gly Ile Lys Tyr Leu Leu Thr Tyr Val Trp Gln Thr Asn Asp Asp 180 185 190 Ile Phe Arg Val Ser Ala Lys Phe Asn Ile Ser Ala Leu Asn Ile Ser 195 200 205 Gly Ala Asn Asn Phe Asp Asn Gly Ser Pro Val Val Gly Gln Pro Val 210 215 220 Leu Ile Pro Leu Thr Lys Leu Pro Ala Leu Ser Gln Pro Leu Pro Pro 225 230 235 240 His Gly Lys His Ile Phe Lys His Arg Leu Met Leu Ile Val Ile Ile 245 250 255 Cys Leu Gly Val Ala Leu Ser Val Ala Ser Leu Leu Ala Ile Phe Leu 260 265 270 Val His Thr His Arg Leu Arg Lys Arg Gln Lys Leu Leu Asn Asp Lys 275 280 285 Ser Leu Ser Leu Glu Ser Ala Glu Trp Phe Arg Met Lys Glu Gly Lys 290 295 300 Ser Glu Glu Lys Ile Glu Met Lys Phe Ile Gln Asp Lys Leu Leu Pro 305 310 315 320 Gly Val Ser Ser Tyr Leu Gly Lys Ala Ile Leu Tyr Asp Val Lys Thr 325 330 335 Ile Met Glu Ala Thr Met Asn Leu Asn Asp His Cys Gly Ile Gly Gly 340 345 350 Ser Val Tyr Arg Ala Val Ile Asp Gly Lys Val Leu Ala Val Lys Lys 355 360 365 Thr Lys Glu Asp Val Thr Glu Glu Leu Asn Ile Leu Gln Lys Val Asn 370 375 380 His Ala Asn Leu Val Lys Leu Met Gly Ile Ser Ser Glu Ile Asp Gly 385 390 395 400 Val Arg Phe Leu Val Tyr Glu Tyr Ala Glu Asn Gly Ser Leu Asp Lys 405 410 415 Trp Leu Tyr His Lys Thr Ser Thr Asn Ser Ser Ser Gly Ala Phe Leu 420 425 430 Thr Trp Ser Gln Arg Leu Ser Ile Ala Leu Asp Val Ala Asn Gly Leu 435 440 445 Gln Tyr Leu His Glu His Thr Gln Pro Ser Ile Val His Met Asp Ile 450 455 460 Arg Thr Ser Asn Ile Leu Leu Asp Ser Lys Tyr Lys Ala Lys Ile Ala 465 470 475 480 Asn Phe Ser Met Ala Arg Thr Ala Ala Asn Ser Val Thr Pro Lys Val 485 490 495 Asp Val Phe Ser Phe Gly Val Ile Leu Leu Ser Leu Leu Ser Gly Lys 500 505 510 Lys Gly Met Glu Thr Thr Asp Asn Gly Glu Val Ile Met Leu Trp Lys 515 520 525 Asp Val Arg Gly Val Leu Glu Ala Glu Glu Lys Lys Gln Glu Lys Leu 530 535 540 Arg Ala Trp Met Asp Pro Thr Leu Glu Ser Phe Tyr Pro Ile Asp Gly 545 550 555 560 Ala Leu Ser Leu Thr Ala Leu Ala Ser Ala Cys Thr Gln Glu Lys Ser 565 570 575 Ser Ala Arg Pro Ser Met Ala Glu Val Val Phe Asn Leu Ser Val Leu 580 585 590 Thr His Ser Ser Ser Glu Ser Thr Leu Glu Arg Ser Trp Asn Ser Ala 595 600 605 Leu Glu Val Glu Glu Val Leu Gln Thr Ile Ser Pro Ile Lys Ala Arg 610 615 620 24 1317 DNA 百脉根 24 ggacatgaga ttgaagctcc aaaattagct cttttttctg atgaatactt aatgctttgt 60 tgtattcact tgattaagtg ctagaaatca tctttgcatg atcatagatt aaatgaattt 120 ccagttggtg tgtggagagc tattttgtta tgctgacatc tgcaatttgc agggcatcta 180 atgattgtca tttcttaaat tattattggt tgtttccgtt tctttaatta tctgttttaa 240 tcttgcaggt catacaaatt aaaatactag ccaccaccca agacatacta aatggggtag 300 tagagggaag ggtaaggtcg ataaggatga ctttttattc tataaaattt aggagaattt 360 gagcttaagt ggcaaggcaa acgacattac tatacgaatt ggctttgtac cagaaacagg 420 gaacaaataa tattttacaa ataagctatt atcatgtcag ctcatttgtt caactttgat 480 ttgattaaaa attaaatgaa gttgaatttg ttgagctgct ttattatata tgccactgga 540 tgtttccgca ttctaagtgc atgtttgaaa acatttctac aattgattac gaaggaaaaa 600 ttaatcatgg agagaagctt atgtgcgtag cttctgtatt tctgaattga ttctatctgt 660 acagtagcat ttagataatg aatgatcttg gttctcgcta agcatcaaac caatctctac 720 ccttttaaaa ttgcaagaat tataagtcat gcattgaccc aaatccttct gtggttatgc 780 cccttaaaaa tccggcaaga catcaagtta gttggtcatt agggttccac cagctagctg 840 acaccttgta caacaactgg ccgtcctaaa gttgggtaag cattacaata ctaaatgcca 900 ttttattata ttttgcgcat ggttatatac ctaagtagga tttgtccaca gtttctttga 960 ttcggaaagg aaaaaatatt tagttgacac tgacagaagc agattttata tacatatatt 1020 atgaaatgac tcctacatga gatacacgaa tctcatcccc atgagttgca gtttgacaga 1080 gtacacactt atcaacttgc tggaatatag gaaagtctaa ccaatgatgt cgatccgtat 1140 tgccttaatt ttggtaaatt tagtattaca tgatcattat tgatatacta aaccacagga 1200 tattttattg acaatgtgaa tgttccatat tttcaacaat gctgattccc tctgataaag 1260 aacaagttcc ttttctcttt ccctgttaac tatcatttgt tccccacttc acaaaca 1317 25 1317 DNA 百脉根 25 ggacatgaga ttgaagctcc aaaattagct cttttttctg atgaatactt aatgctttgt 60 tgtattcact tgattaagtg ctagaaatca tctttgcatg atcatagatt aaatgaattt 120 ccagttggtg tgtggagagc tattttgtta tgctgacatc tgcaatttgc agggcatcta 180 atgattgtca tttcttaaat tattattggt tgtttccgtt tctttaatta tctgttttaa 240 tcttgcaggt catacaaatt aaaatactag ccaccaccca agacatacta aatggggtag 300 tagagggaag ggtaaggtcg ataaggatga ctttttattc tataaaattt aggagaattt 360 gagcttaagt ggcaaggcaa acgacattac tatacgaatt ggctttgtac cagaaacagg 420 gaacaaataa tattttacaa ataagctatt atcatgtcag ctcatttgtt caactttgat 480 ttgattaaaa attaaatgaa gttgaatttg ttgagctgct ttattatata tgccactgga 540 tgtttccgca ttctaagtgc atgtttgaaa acatttctac aattgattac gaaggaaaaa 600 ttaatcatgg agagaagctt atgtgcgtag cttctgtatt tctgaattga ttctatctgt 660 acagtagcat ttagataatg aatgatcttg gttctcgcta agcatcaaac caatctctac 720 ccttttaaaa ttgcaagaat tataagtcat gcattgaccc aaatccttct gtggttatgc 780 cccttaaaaa tccggcaaga catcaagtta gttggtcatt agggttccac cagctagctg 840 acaccttgta caacaactgg ccgtcctaaa gttgggtaag cattacaata ctaaatgcca 900 ttttattata ttttgcgcat ggttatatac ctaagtagga tttgtccaca gtttctttga 960 ttcggaaagg aaaaaatatt tagttgacac tgacagaagc agattttata tacatatatt 1020 atgaaatgac tcctacatga gatacacgaa tctcatcccc atgagttgca gtttgacaga 1080 gtacacactt atcaacttgc tggaatatag gaaagtctaa ccaatgatgt cgatccgtat 1140 tgccttaatt ttggtaaatt tagtattaca tgatcattat tgatatacta aaccacagga 1200 tattttattg acaatgtgaa tgttccatat tttcaacaat gctgattccc tctgataaag 1260 aacaagttcc ttttctcttt ccctgttaac tatcatttgt tccccacttc acaaaca 1317 26 61 PRT 蒺藜苜蓿 26 Cys Thr Lys Asn His Ser Phe Ala Asn Ile Thr Tyr Ser Ile Lys Gln 1 5 10 15 Gly Asp Asn Phe Phe Ile Leu Ser Ile Thr Ser Tyr Gln Asn Leu Thr 20 25 30 Asn Tyr Leu Glu Phe Lys Asn Phe Asn Pro Asn Leu Ser Pro Thr Leu 35 40 45 Leu Pro Leu Asp Thr Lys Val Ser Val Pro Leu Phe Cys 50 55 60 27 61 PRT 百脉根 27 Cys Ala Gly Asn His Ser Ser Ala Asn Thr Ser Tyr Gln Ile Gln Leu 1 5 10 15 Gly Asp Ser Tyr Asp Phe Val Ala Thr Thr Leu Tyr Glu Asn Leu Thr 20 25 30 Asn Trp Asn Ile Val Gln Ala Ser Asn Pro Gly Val Asn Pro Tyr Leu 35 40 45 Leu Pro Glu Arg Val Lys Val Val Phe Pro Leu Phe Cys 50 55 60 28 233 PRT 人工序列 合成构建体 28 Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala Ala His 1 5 10 15 Ser Ala Phe Ala Gln Gly Gly Ala Gly Asn Gly Thr Gly Arg Phe Ala 20 25 30 Cys Val Val Pro Ala Pro Cys Asp Thr Phe Val Leu Tyr Arg Thr Gln 35 40 45 Ser Pro Gly Ser Leu Asp Leu Gly Ala Ile Ser Asp Leu Phe Gly Val 50 55 60 Ser Arg Ala Met Ile Ala Ala Ala Asn Asn Leu Ser Leu Ile Asp Glu 65 70 75 80 Asp Ala Ala Leu Leu Pro Asp Gln Pro Leu Leu Val Pro Val Arg Cys 85 90 95 Gly Cys Thr Gly Asn Arg Ser Phe Val Asn Val Thr Tyr Pro Ile His 100 105 110 Ser Gly Asp Thr Phe Tyr Ala Leu Ala Leu Thr Gly Tyr Glu Asn Leu 115 120 125 Thr Thr Pro Asp Val Ile Gln Glu Leu Asn Pro Gln Ala Val Phe Asn 130 135 140 Lys Leu Asn Val Ser Gln Leu Val Thr Val Pro Leu Phe Cys Arg Cys 145 150 155 160 Pro Thr Pro Ala Glu Arg Ser Ala Gly Val Leu Gln Gln Ile Thr Tyr 165 170 175 Met Trp Arg Pro Val Asp Thr Met Ser Arg Val Ser Lys Leu Met Gly 180 185 190 Ser Asp Ala Ser Ala Ile Ala Ala Ala Asn Asn Val Ser Ala Asp Phe 195 200 205 Thr Ser Thr Thr Met Leu Pro Met Leu Ile Pro Val Ala Arg Pro Pro 210 215 220 Val Leu Pro His His His His His His 225 230 29 207 PRT 人工序列 合成构建体 29 Gln Gly Gly Ala Gly Asn Gly Thr Gly Arg Phe Ala Cys Val Val Pro 1 5 10 15 Ala Pro Cys Asp Thr Phe Val Leu Tyr Arg Thr Gln Ser Pro Gly Ser 20 25 30 Leu Asp Leu Gly Ala Ile Ser Asp Leu Phe Gly Val Ser Arg Ala Met 35 40 45 Ile Ala Ala Ala Asn Asn Leu Ser Leu Ile Asp Glu Asp Ala Ala Leu 50 55 60 Leu Pro Asp Gln Pro Leu Leu Val Pro Val Arg Cys Gly Cys Thr Gly 65 70 75 80 Asn Arg Ser Phe Val Asn Val Thr Tyr Pro Ile His Ser Gly Asp Thr 85 90 95 Phe Tyr Ala Leu Ala Leu Thr Gly Tyr Glu Asn Leu Thr Thr Pro Asp 100 105 110 Val Ile Gln Glu Leu Asn Pro Gln Ala Val Phe Asn Lys Leu Asn Val 115 120 125 Ser Gln Leu Val Thr Val Pro Leu Phe Cys Arg Cys Pro Thr Pro Ala 130 135 140 Glu Arg Ser Ala Gly Val Leu Gln Gln Ile Thr Tyr Met Trp Arg Pro 145 150 155 160 Val Asp Thr Met Ser Arg Val Ser Lys Leu Met Gly Ser Asp Ala Ser 165 170 175 Ala Ile Ala Ala Ala Asn Asn Val Ser Ala Asp Phe Thr Ser Thr Thr 180 185 190 Met Leu Pro Met Leu Ile Pro Val Ala Arg Pro Pro Val Leu Pro 195 200 205 30 622 PRT 百脉根 30 Met Glu His Pro Arg Leu Gly Phe Pro Ile Thr Leu Leu Leu Phe Ser 1 5 10 15 Phe Ile Leu Leu Pro Ser Thr Ser Gln Ser Lys Cys Thr His Gly Cys 20 25 30 Ala Leu Ala Gln Ala Ser Tyr Tyr Leu Leu Asn Gly Ser Asn Leu Thr 35 40 45 Tyr Ile Ser Glu Ile Met Gln Ser Ser Leu Leu Thr Lys Pro Glu Asp 50 55 60 Ile Val Ser Tyr Asn Gln Asp Thr Ile Ala Ser Lys Asp Ser Val Gln 65 70 75 80 Ala Gly Gln Arg Ile Asn Val Pro Phe Pro Cys Asp Cys Ile Glu Gly 85 90 95 Glu Phe Leu Gly His Thr Phe Gln Tyr Asp Val Gln Lys Gly Asp Arg 100 105 110 Tyr Asp Thr Ile Ala Gly Thr Asn Tyr Ala Asn Leu Thr Thr Val Glu 115 120 125 Trp Leu Arg Arg Phe Asn Ser Tyr Pro Pro Asp Asn Ile Pro Asp Thr 130 135 140 Gly Thr Leu Asn Val Thr Val Asn Cys Ser Cys Gly Asp Ser Gly Val 145 150 155 160 Gly Asp Tyr Gly Leu Phe Val Thr Tyr Pro Leu Arg Pro Gly Glu Thr 165 170 175 Leu Gly Ser Val Ala Ser Asn Val Lys Leu Asp Ser Ala Leu Leu Gln 180 185 190 Lys Tyr Asn Pro Asn Val Asn Phe Asn Gln Gly Ser Gly Ile Val Tyr 195 200 205 Ile Pro Ala Lys Asp Gln Asn Gly Ser Tyr Val Leu Leu Gly Ser Ser 210 215 220 Ser Gly Gly Leu Ala Gly Gly Ala Ile Ala Gly Ile Ala Ala Gly Val 225 230 235 240 Ala Val Cys Leu Leu Leu Leu Ala Gly Phe Ile Tyr Val Gly Tyr Phe 245 250 255 Arg Lys Lys Arg Ile Gln Lys Glu Glu Leu Leu Ser Gln Glu Thr Arg 260 265 270 Ala Ile Phe Pro Gln Asp Gly Lys Asp Glu Asn Pro Arg Ser Thr Val 275 280 285 Asn Glu Thr Pro Gly Pro Gly Gly Pro Ala Ala Met Ala Gly Ile Thr 290 295 300 Val Asp Lys Ser Val Glu Phe Ser Tyr Asp Glu Leu Ala Thr Ala Thr 305 310 315 320 Asp Asn Phe Ser Leu Ala Asn Lys Ile Gly Gln Gly Gly Phe Gly Ser 325 330 335 Val Tyr Tyr Ala Glu Leu Arg Gly Glu Arg Ala Ala Ile Lys Lys Met 340 345 350 Asp Met Gln Ala Ser Lys Glu Phe Leu Ala Glu Leu Lys Val Leu Thr 355 360 365 Arg Val His His Leu Asn Leu Val Arg Leu Ile Gly Tyr Ser Ile Glu 370 375 380 Gly Ser Leu Phe Leu Val Tyr Glu Phe Ile Glu Asn Gly Asn Leu Ser 385 390 395 400 Gln His Leu Arg Gly Ser Gly Arg Asp Pro Leu Pro Trp Ala Thr Arg 405 410 415 Val Gln Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu Tyr Ile His Glu 420 425 430 His Thr Val Pro Val Tyr Ile His Arg Asp Ile Lys Ser Ala Asn Ile 435 440 445 Leu Ile Asp Lys Asn Tyr Arg Gly Lys Val Ala Asp Phe Gly Leu Thr 450 455 460 Lys Leu Thr Glu Val Gly Ser Ser Ser Leu Pro Thr Gly Arg Leu Val 465 470 475 480 Gly Thr Phe Gly Tyr Met Pro Pro Glu Tyr Ala Gln Tyr Gly Asp Val 485 490 495 Ser Pro Lys Val Asp Val Tyr Ala Phe Gly Val Val Leu Tyr Glu Leu 500 505 510 Ile Ser Ala Lys Asp Ala Ile Val Lys Thr Ser Glu Ser Ile Thr Asp 515 520 525 Ser Lys Gly Leu Val Ala Leu Phe Glu Gly Val Leu Ser Gln Pro Asp 530 535 540 Pro Thr Glu Asp Leu Arg Lys Leu Val Asp Gln Arg Leu Gly Asp Asn 545 550 555 560 Tyr Pro Val Asp Ser Val Arg Lys Met Ala Gln Leu Ala Lys Ala Cys 565 570 575 Thr Gln Asp Asn Pro Gln Leu Arg Pro Ser Met Arg Ser Ile Val Val 580 585 590 Ala Leu Met Thr Leu Ser Ser Thr Thr Asp Asp Trp Asp Val Gly Ser 595 600 605 Phe Tyr Glu Asn Gln Asn Leu Val Asn Leu Met Ser Gly Arg 610 615 620 31 621 PRT 蒺藜苜蓿 31 Met Glu His Gln Pro Arg Phe Thr Ser Phe Ile Ser Leu Pro Leu Phe 1 5 10 15 Ser Ile Phe Leu Ala Ser Ile Pro Phe Ile Thr Glu Ser Lys Cys Thr 20 25 30 Lys Gly Cys Ser Leu Ala Leu Ala Asn Phe Tyr Val Ser Gln Gly Ser 35 40 45 Asn Leu Thr Tyr Ile Ser Ser Ile Met Arg Ser Asn Ile Gln Thr Arg 50 55 60 Pro Glu Asp Ile Val Glu Tyr Ser Arg Glu Ile Ile Pro Ser Lys Asp 65 70 75 80 Ser Val Gln Ala Gly Gln Arg Leu Asn Val Pro Phe Pro Cys Asp Cys 85 90 95 Ile Asp Gly Gln Phe Leu Gly His Lys Phe Ser Tyr Asp Val Glu Thr 100 105 110 Gly Asp Thr Tyr Glu Thr Val Ala Thr Asn Asn Tyr Ala Asn Leu Thr 115 120 125 Asn Val Glu Trp Leu Arg Arg Phe Asn Thr Tyr Pro Pro Asn Asp Ile 130 135 140 Pro Asp Thr Gly Thr Leu Asn Val Thr Val Asn Cys Ser Cys Gly Asp 145 150 155 160 Ala Asp Val Gly Asn Tyr Ala Leu Phe Val Thr Tyr Pro Leu Arg Pro 165 170 175 Gly Glu Thr Leu Val Ser Val Ala Asn Ser Ser Lys Val Asp Ser Ser 180 185 190 Leu Leu Gln Arg Tyr Asn Pro Gly Val Asn Phe Asn Gln Gly Ser Gly 195 200 205 Ile Val Phe Val Pro Gly Lys Asp Gln Asn Gly Ser Phe Val Phe Leu 210 215 220 Gly Ser Ser Ser Gly Leu Gly Gly Gly Ala Ile Gly Gly Ile Ala Val 225 230 235 240 Gly Ile Val Val Val Leu Leu Leu Val Ala Ala Ala Ile Tyr Phe Gly 245 250 255 Tyr Phe Arg Lys Lys Lys Ile Gln Lys Glu Glu Leu Phe Ser Arg Asp 260 265 270 Ser Thr Ala Leu Phe Ser Gln Asp Gly Lys Asp Glu Asn Ser His Gly 275 280 285 Ala Ala Asn Val Thr Gln Arg Pro Gly Val Met Thr Gly Ile Thr Val 290 295 300 Asp Lys Ser Val Glu Phe Ser Tyr Asp Glu Leu Ala Ala Ala Ser Asp 305 310 315 320 Asn Phe Ser Met Ala Asn Lys Ile Gly Gln Gly Gly Phe Gly Ser Val 325 330 335 Tyr Tyr Ala Glu Leu Arg Gly Glu Lys Ala Ala Ile Lys Lys Met Asp 340 345 350 Met Gln Ala Thr Lys Glu Phe Leu Ala Glu Leu Lys Val Leu Thr Arg 355 360 365 Val His His Leu Asn Leu Val Arg Leu Ile Gly Tyr Ser Ile Glu Gly 370 375 380 Ser Leu Phe Leu Val Tyr Glu Tyr Ile Glu Asn Gly Asn Leu Ser Gln 385 390 395 400 His Leu Arg Gly Ser Gly Arg Asp Pro Leu Pro Trp Ala Thr Arg Val 405 410 415 Gln Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu Tyr Ile His Glu His 420 425 430 Thr Val Pro Val Tyr Ile His Arg Asp Ile Lys Pro Ala Asn Ile Leu 435 440 445 Ile Asp Lys Asn Phe Arg Gly Lys Val Ala Asp Phe Gly Leu Thr Lys 450 455 460 Leu Thr Glu Val Gly Ser Ser Ser Leu Pro Thr Gly Arg Leu Val Gly 465 470 475 480 Thr Phe Gly Tyr Met Pro Pro Glu Tyr Ala Gln Tyr Gly Asp Val Ser 485 490 495 Pro Lys Val Asp Val Tyr Ala Phe Gly Val Val Leu Tyr Glu Leu Ile 500 505 510 Ser Ala Lys Glu Ala Ile Val Lys Ser Ser Glu Ser Val Ala Asp Ser 515 520 525 Lys Gly Leu Val Gly Leu Phe Glu Gly Val Leu Ser Gln Pro Asp Pro 530 535 540 Thr Glu Asp Leu Arg Lys Ile Val Asp Pro Arg Leu Gly Asp Asn Tyr 545 550 555 560 Pro Ala Asp Ser Val Arg Lys Met Ala Gln Leu Ala Lys Ala Cys Thr 565 570 575 Gln Glu Asn Pro Gln Leu Arg Pro Ser Met Arg Ser Ile Val Val Ala 580 585 590 Leu Met Thr Leu Ser Ser Thr Thr Asp Asp Trp Asp Val Gly Ser Phe 595 600 605 Tyr Glu Asn Gln Asn Leu Val Asn Leu Met Ser Gly Arg 610 615 620 32 623 PRT 百脉根 32 Met Lys Leu Lys Thr Gly Leu Leu Leu Phe Phe Ile Leu Leu Leu Gly 1 5 10 15 His Val Cys Phe His Val Glu Ser Asn Cys Leu Lys Gly Cys Asp Leu 20 25 30 Ala Leu Ala Ser Tyr Tyr Ile Leu Pro Gly Val Phe Ile Leu Gln Asn 35 40 45 Ile Thr Thr Phe Met Gln Ser Glu Ile Val Ser Ser Asn Asp Ala Ile 50 55 60 Thr Ser Tyr Asn Lys Asp Lys Ile Leu Asn Asp Ile Asn Ile Gln Ser 65 70 75 80 Phe Gln Arg Leu Asn Ile Pro Phe Pro Cys Asp Cys Ile Gly Gly Glu 85 90 95 Phe Leu Gly His Val Phe Glu Tyr Ser Ala Ser Lys Gly Asp Thr Tyr 100 105 110 Glu Thr Ile Ala Asn Leu Tyr Tyr Ala Asn Leu Thr Thr Val Asp Leu 115 120 125 Leu Lys Arg Phe Asn Ser Tyr Asp Pro Lys Asn Ile Pro Val Asn Ala 130 135 140 Lys Val Asn Val Thr Val Asn Cys Ser Cys Gly Asn Ser Gln Val Ser 145 150 155 160 Lys Asp Tyr Gly Leu Phe Ile Thr Tyr Pro Ile Arg Pro Gly Asp Thr 165 170 175 Leu Gln Asp Ile Ala Asn Gln Ser Ser Leu Asp Ala Gly Leu Ile Gln 180 185 190 Ser Phe Asn Pro Ser Val Asn Phe Ser Lys Asp Ser Gly Ile Ala Phe 195 200 205 Ile Pro Gly Arg Tyr Lys Asn Gly Val Tyr Val Pro Leu Tyr His Arg 210 215 220 Thr Ala Gly Leu Ala Ser Gly Ala Ala Val Gly Ile Ser Ile Ala Gly 225 230 235 240 Thr Phe Val Leu Leu Leu Leu Ala Phe Cys Met Tyr Val Arg Tyr Gln 245 250 255 Lys Lys Glu Glu Glu Lys Ala Lys Leu Pro Thr Asp Ile Ser Met Ala 260 265 270 Leu Ser Thr Gln Asp Gly Asn Ala Ser Ser Ser Ala Glu Tyr Glu Thr 275 280 285 Ser Gly Ser Ser Gly Pro Gly Thr Ala Ser Ala Thr Gly Leu Thr Ser 290 295 300 Ile Met Val Ala Lys Ser Met Glu Phe Ser Tyr Gln Glu Leu Ala Lys 305 310 315 320 Ala Thr Asn Asn Phe Ser Leu Asp Asn Lys Ile Gly Gln Gly Gly Phe 325 330 335 Gly Ala Val Tyr Tyr Ala Glu Leu Arg Gly Lys Lys Thr Ala Ile Lys 340 345 350 Lys Met Asp Val Gln Ala Ser Thr Glu Phe Leu Cys Glu Leu Lys Val 355 360 365 Leu Thr His Val His His Leu Asn Leu Val Arg Leu Ile Gly Tyr Cys 370 375 380 Val Glu Gly Ser Leu Phe Leu Val Tyr Glu His Ile Asp Asn Gly Asn 385 390 395 400 Leu Gly Gln Tyr Leu His Gly Ser Gly Lys Glu Pro Leu Pro Trp Ser 405 410 415 Ser Arg Val Gln Ile Ala Leu Asp Ala Ala Arg Gly Leu Glu Tyr Ile 420 425 430 His Glu His Thr Val Pro Val Tyr Ile His Arg Asp Val Lys Ser Ala 435 440 445 Asn Ile Leu Ile Asp Lys Asn Leu Arg Gly Lys Val Ala Asp Phe Gly 450 455 460 Leu Thr Lys Leu Ile Glu Val Gly Asn Ser Thr Leu Gln Thr Arg Leu 465 470 475 480 Val Gly Thr Phe Gly Tyr Met Pro Pro Glu Tyr Ala Gln Tyr Gly Asp 485 490 495 Ile Ser Pro Lys Ile Asp Val Tyr Ala Phe Gly Val Val Leu Phe Glu 500 505 510 Leu Ile Ser Ala Lys Asn Ala Val Leu Lys Thr Gly Glu Leu Val Ala 515 520 525 Glu Ser Lys Gly Leu Val Ala Leu Phe Glu Glu Ala Leu Asn Lys Ser 530 535 540 Asp Pro Cys Asp Ala Leu Arg Lys Leu Val Asp Pro Arg Leu Gly Glu 545 550 555 560 Asn Tyr Pro Ile Asp Ser Val Leu Lys Ile Ala Gln Leu Gly Arg Ala 565 570 575 Cys Thr Arg Asp Asn Pro Leu Leu Arg Pro Ser Met Arg Ser Leu Val 580 585 590 Val Ala Leu Met Thr Leu Ser Ser Leu Thr Glu Asp Cys Asp Asp Glu 595 600 605 Ser Ser Tyr Glu Ser Gln Thr Leu Ile Asn Leu Leu Ser Val Arg 610 615 620 33 620 PRT 蒺藜苜蓿 33 Met Asn Leu Lys Asn Gly Leu Leu Leu Phe Ile Leu Phe Leu Asp Cys 1 5 10 15 Val Phe Phe Lys Val Glu Ser Lys Cys Val Lys Gly Cys Asp Val Ala 20 25 30 Leu Ala Ser Tyr Tyr Ile Ile Pro Ser Ile Gln Leu Arg Asn Ile Ser 35 40 45 Asn Phe Met Gln Ser Lys Ile Val Leu Thr Asn Ser Phe Asp Val Ile 50 55 60 Met Ser Tyr Asn Arg Asp Val Val Phe Asp Lys Ser Gly Leu Ile Ser 65 70 75 80 Tyr Thr Arg Ile Asn Val Pro Phe Pro Cys Glu Cys Ile Gly Gly Glu 85 90 95 Phe Leu Gly His Val Phe Glu Tyr Thr Thr Lys Glu Gly Asp Asp Tyr 100 105 110 Asp Leu Ile Ala Asn Thr Tyr Tyr Ala Ser Leu Thr Thr Val Glu Leu 115 120 125 Leu Lys Lys Phe Asn Ser Tyr Asp Pro Asn His Ile Pro Val Lys Ala 130 135 140 Lys Ile Asn Val Thr Val Ile Cys Ser Cys Gly Asn Ser Gln Ile Ser 145 150 155 160 Lys Asp Tyr Gly Leu Phe Val Thr Tyr Pro Leu Arg Ser Asp Asp Thr 165 170 175 Leu Ala Lys Ile Ala Thr Lys Ala Gly Leu Asp Glu Gly Leu Ile Gln 180 185 190 Asn Phe Asn Gln Asp Ala Asn Phe Ser Ile Gly Ser Gly Ile Val Phe 195 200 205 Ile Pro Gly Arg Asp Gln Asn Gly His Phe Phe Pro Leu Tyr Ser Arg 210 215 220 Thr Gly Ile Ala Lys Gly Ser Ala Val Gly Ile Ala Met Ala Gly Ile 225 230 235 240 Phe Gly Leu Leu Leu Phe Val Ile Tyr Ile Tyr Ala Lys Tyr Phe Gln 245 250 255 Lys Lys Glu Glu Glu Lys Thr Lys Leu Pro Gln Thr Ser Arg Ala Phe 260 265 270 Ser Thr Gln Asp Ala Ser Gly Ser Ala Glu Tyr Glu Thr Ser Gly Ser 275 280 285 Ser Gly His Ala Thr Gly Ser Ala Ala Gly Leu Thr Gly Ile Met Val 290 295 300 Ala Lys Ser Thr Glu Phe Thr Tyr Gln Glu Leu Ala Lys Ala Thr Asn 305 310 315 320 Asn Phe Ser Leu Asp Asn Lys Ile Gly Gln Gly Gly Phe Gly Ala Val 325 330 335 Tyr Tyr Ala Glu Leu Arg Gly Glu Lys Thr Ala Ile Lys Lys Met Asp 340 345 350 Val Gln Ala Ser Ser Glu Phe Leu Cys Glu Leu Lys Val Leu Thr His 355 360 365 Val His His Leu Asn Leu Val Arg Leu Ile Gly Tyr Cys Val Glu Gly 370 375 380 Ser Leu Phe Leu Val Tyr Glu His Ile Asp Asn Gly Asn Leu Gly Gln 385 390 395 400 Tyr Leu His Gly Ile Gly Thr Glu Pro Leu Pro Trp Ser Ser Arg Val 405 410 415 Gln Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu Tyr Ile His Glu His 420 425 430 Thr Val Pro Val Tyr Ile His Arg Asp Val Lys Ser Ala Asn Ile Leu 435 440 445 Ile Asp Lys Asn Leu Arg Gly Lys Val Ala Asp Phe Gly Leu Thr Lys 450 455 460 Leu Ile Glu Val Gly Asn Ser Thr Leu His Thr Arg Leu Val Gly Thr 465 470 475 480 Phe Gly Tyr Met Pro Pro Glu Tyr Ala Gln Tyr Gly Asp Val Ser Pro 485 490 495 Lys Ile Asp Val Tyr Ala Phe Gly Val Val Leu Tyr Glu Leu Ile Thr 500 505 510 Ala Lys Asn Ala Val Leu Lys Thr Gly Glu Ser Val Ala Glu Ser Lys 515 520 525 Gly Leu Val Gln Leu Phe Glu Glu Ala Leu His Arg Met Asp Pro Leu 530 535 540 Glu Gly Leu Arg Lys Leu Val Asp Pro Arg Leu Lys Glu Asn Tyr Pro 545 550 555 560 Ile Asp Ser Val Leu Lys Met Ala Gln Leu Gly Arg Ala Cys Thr Arg 565 570 575 Asp Asn Pro Leu Leu Arg Pro Ser Met Arg Ser Ile Val Val Ala Leu 580 585 590 Met Thr Leu Ser Ser Pro Thr Glu Asp Cys Asp Asp Asp Ser Ser Tyr 595 600 605 Glu Asn Gln Ser Leu Ile Asn Leu Leu Ser Thr Arg 610 615 620 34 617 PRT 玉米 34 Met Ala Arg Ile Leu Met Arg Leu Leu Leu Leu Ala Ala Ala Ala Ala 1 5 10 15 Val Ala Ala Gly Asp Gly Cys Leu Asn Ser Gly Cys Val Ala Leu Gly 20 25 30 Ser Tyr Leu Val Ala Arg Asn Gln Asn Leu Thr Tyr Ile Ala Ser Leu 35 40 45 Phe Gly Ile Gly Asp Tyr His Ala Leu Ala Arg Tyr Asn Pro Gly Thr 50 55 60 Thr Asn Leu Asp Tyr Ile Gln Ala Gly Gln Ser Val Asn Ile Ser Phe 65 70 75 80 Thr Cys Gly Cys His Thr Phe Pro Asn Ser Asp Ala Thr Tyr Leu Gly 85 90 95 Gly Ser Phe Pro His Lys Val Val Thr Gly Asp Thr Tyr Gly Gly Ile 100 105 110 Ala Gln Asn Tyr Asn Asn Leu Thr Ser Ala Ala Trp Leu Ala Val Thr 115 120 125 Asn Pro Tyr Pro Thr Asn Asn Ile Pro Asp Thr Asn Thr Val Val Asn 130 135 140 Val Thr Val Asn Cys Thr Cys Gly Asp Pro Lys Ile Ser Ser Asp Tyr 145 150 155 160 Gly Phe Phe Leu Thr Tyr Pro Leu Met Gly Gln Thr Leu Ala Ala Val 165 170 175 Ala Ala Asn Tyr Ser Phe Asn Ser Ser Ser Gln Leu Asp Leu Leu Arg 180 185 190 Lys Tyr Asn Pro Gly Met Asp Thr Ala Thr Ser Gly Leu Val Phe Ile 195 200 205 Pro Val Lys Asp Gly Asn Gly Ser Tyr His Pro Leu Lys Pro Pro Gly 210 215 220 Asn Gly Gly Ser Ile Gly Ala Ile Val Gly Gly Val Val Gly Gly Val 225 230 235 240 Ala Ile Leu Val Leu Gly Val Leu Leu Tyr Ile Met Phe Tyr Arg Arg 245 250 255 Lys Lys Ala Asn Lys Ala Ala Leu Leu Pro Ser Ser Glu Asp Ser Thr 260 265 270 Gln Leu Ala Thr Thr Ser Met Asp Lys Ser Ala Leu Ser Thr Ser Gln 275 280 285 Ala Asp Ser Ser Ser Gly Val Pro Gly Ile Thr Val Asp Lys Ser Val 290 295 300 Glu Phe Ser Tyr Glu Glu Leu Phe Asn Ala Thr Glu Gly Phe Ser Met 305 310 315 320 Ser Asn Lys Ile Gly Gln Gly Gly Phe Gly Ala Val Tyr Tyr Ala Glu 325 330 335 Leu Arg Gly Glu Lys Ala Ala Ile Lys Lys Met Asp Met Gln Ala Ser 340 345 350 His Glu Phe Leu Ala Glu Leu Lys Val Leu Thr His Val His His Leu 355 360 365 Asn Leu Val Arg Leu Ile Gly Phe Cys Thr Glu Ser Ser Leu Phe Leu 370 375 380 Val Tyr Glu Phe Ile Glu Asn Gly Asn Leu Ser Gln His Leu Arg Gly 385 390 395 400 Thr Gly Tyr Glu Pro Leu Ser Trp Ala Ala Arg Val Gln Ile Ala Leu 405 410 415 Asp Ser Ala Arg Gly Leu Glu Tyr Ile His Glu His Thr Val Pro Val 420 425 430 Tyr Ile His Arg Asp Ile Lys Ser Ala Asn Ile Leu Ile Asp Lys Asn 435 440 445 Tyr Arg Ala Lys Val Ala Asp Phe Gly Leu Thr Lys Leu Thr Glu Val 450 455 460 Gly Asn Thr Ser Leu Pro Thr Arg Gly Ile Val Gly Thr Phe Gly Tyr 465 470 475 480 Met Pro Pro Glu Tyr Ala Arg Tyr Gly Asp Val Ser Pro Lys Val Asp 485 490 495 Val Tyr Ala Phe Gly Val Val Leu Tyr Glu Leu Ile Ser Ala Lys Asp 500 505 510 Ala Ile Val Arg Ser Thr Glu Ser Ser Ser Asp Ser Lys Gly Leu Val 515 520 525 Tyr Leu Phe Glu Glu Ala Leu Asn Thr Pro Asp Pro Lys Glu Gly Leu 530 535 540 Gln Arg Leu Ile Asp Pro Ala Leu Gly Glu Asp Tyr Pro Ile Asp Ser 545 550 555 560 Ile Leu Lys Met Thr Val Leu Ala Arg Ala Cys Thr Gln Glu Asp Pro 565 570 575 Lys Ala Arg Pro Thr Met Arg Ser Ile Val Val Ala Leu Met Thr Leu 580 585 590 Ser Ser Thr Ser Glu Phe Trp Asp Met Asn Ala Ile Gln Glu Asn Gln 595 600 605 Gly Val Val Asn Leu Met Ser Gly Arg 610 615 35 626 PRT 大麦 35 Met Glu Ala Pro Leu His Ser Leu Leu Leu Leu Leu Leu Leu Ala Ala 1 5 10 15 Ala Ala Gly Pro Lys Thr Ala Ala Ala Val Gly Asp Gly Cys Ser Arg 20 25 30 Gly Cys Asp Leu Ala Leu Ala Ser Tyr Tyr Ile Ala Pro Asn Gln Asn 35 40 45 Val Thr Tyr Ile Ala Ser Leu Phe Gly Phe Ser Glu Tyr Arg Val Leu 50 55 60 Gly Gln Tyr Asn Pro Gly Val Asn Asn Leu Asp Tyr Val Val Ala Gly 65 70 75 80 Asp Arg Leu Asn Val Ser Leu Thr Cys Lys Cys Leu Ala Ser Leu Ser 85 90 95 Ala Pro Ala Ser Thr Phe Leu Ala Ala Ser Ile Pro Tyr Lys Val Ala 100 105 110 Thr Gly Glu Thr Tyr Leu Arg Ile Ala Asp Asn Tyr Asn Asn Leu Thr 115 120 125 Thr Ala Asp Trp Leu Val Ala Thr Asn Thr Tyr Pro Ala Asn Asn Ile 130 135 140 Pro Asp Val Ala Thr Val Asn Ala Thr Val Asn Cys Ser Cys Gly Asp 145 150 155 160 Ala Gly Ile Ser Thr Asp Tyr Gly Leu Phe Leu Thr Tyr Pro Leu Arg 165 170 175 Asp Arg Glu Thr Leu Ala Ser Val Ala Ala Asn His Gly Phe Ser Ser 180 185 190 Pro Glu Lys Met Asp Leu Leu Lys Lys Tyr Asn Pro Gly Met Asp Gly 195 200 205 Val Thr Gly Ser Gly Ile Val Tyr Ile Pro Ala Lys Asp Pro Asn Gly 210 215 220 Ser Tyr Arg Pro Leu Glu Ser Pro Gly Lys Lys Ser Ser Ala Gly Ala 225 230 235 240 Ile Ala Gly Gly Val Val Ala Gly Val Val Ala Leu Val Leu Gly Val 245 250 255 Val Leu Phe Leu Phe Tyr Arg Arg Arg Lys Ala Lys Lys Asp Ala Leu 260 265 270 Leu Pro Ser Ser Glu Glu Ser Thr Arg Leu Ala Ser Ala Ile Ser Met 275 280 285 Gln Lys Val Thr Pro Ser Thr Ser Gln Ala Asp Gly Ala Ser Pro Ala 290 295 300 Ala Gly Ile Thr Val Asp Lys Ser Val Glu Phe Ser Tyr Glu Glu Leu 305 310 315 320 Phe Asn Ala Thr Glu Gly Phe Asn Ile Ile His Lys Ile Gly Gln Gly 325 330 335 Gly Phe Gly Ala Val Tyr Tyr Ala Glu Leu Arg Gly Glu Lys Ala Ala 340 345 350 Ile Lys Lys Met Asp Met Gln Ala Thr Gln Glu Phe Leu Ala Glu Leu 355 360 365 Lys Val Leu Thr His Val His His Leu Asn Leu Val Arg Leu Ile Gly 370 375 380 Tyr Cys Thr Glu Ser Ser Leu Phe Leu Val Tyr Glu Phe Ile Glu Asn 385 390 395 400 Gly Asn Leu Ser Gln His Leu Arg Gly Thr Gly Tyr Glu Pro Leu Ser 405 410 415 Trp Val Glu Arg Val Gln Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu 420 425 430 Tyr Ile His Glu His Thr Val Pro Val Tyr Ile His Arg Asp Ile Lys 435 440 445 Ser Ala Asn Ile Leu Ile Asp Lys Asn Thr Arg Ala Lys Val Ala Asp 450 455 460 Phe Gly Leu Thr Lys Leu Thr Glu Val Gly Gly Gly Thr Ser Leu Gln 465 470 475 480 Thr Arg Val Val Gly Thr Phe Gly Tyr Met Pro Pro Glu Tyr Ala Arg 485 490 495 Tyr Gly Asp Val Ser Pro Lys Val Asp Val Tyr Ala Phe Gly Val Val 500 505 510 Leu Tyr Glu Leu Ile Ser Ala Lys Asp Ala Ile Val Arg Ser Ala Glu 515 520 525 Ser Thr Ser Asp Ser Lys Gly Leu Val Tyr Leu Phe Glu Glu Ala Leu 530 535 540 Ser Ala Pro Asp Pro Lys Glu Gly Ile Arg Arg Leu Met Asp Pro Lys 545 550 555 560 Leu Gly Asp Asp Tyr Pro Ile Asp Ala Ile Leu Lys Met Thr His Leu 565 570 575 Ala Asn Ala Cys Thr Gln Glu Asp Pro Lys Leu Arg Pro Thr Met Arg 580 585 590 Ser Val Val Val Ala Leu Met Thr Leu Ser Ser Thr Ser Glu Phe Trp 595 600 605 Asp Met Asn Ala Leu Tyr Glu Asn Pro Gly Leu Val Asn Leu Met Ser 610 615 620 Gly Arg 625 36 613 PRT 粳稻组 36 Met Phe Ser Leu Pro Ala Leu Leu Ile Gly Ala Cys Ala Phe Ala Ala 1 5 10 15 Ala Ala Val Ala Ala Ser Gly Asp Gly Cys Arg Ala Gly Cys Ser Leu 20 25 30 Ala Ile Ala Ala Tyr Tyr Phe Ser Glu Gly Ser Asn Leu Thr Phe Ile 35 40 45 Ala Thr Ile Phe Ala Ile Gly Gly Gly Gly Tyr Gln Ala Leu Leu Pro 50 55 60 Tyr Asn Pro Ala Ile Thr Asn Pro Asp Tyr Val Val Thr Gly Asp Arg 65 70 75 80 Val Leu Val Pro Phe Pro Cys Ser Cys Leu Gly Leu Pro Ala Ala Pro 85 90 95 Ala Ser Thr Phe Leu Ala Gly Ala Ile Pro Tyr Pro Leu Pro Leu Pro 100 105 110 Arg Gly Gly Gly Asp Thr Tyr Asp Ala Val Ala Ala Asn Tyr Ala Asp 115 120 125 Leu Thr Thr Ala Ala Trp Leu Glu Ala Thr Asn Ala Tyr Pro Pro Gly 130 135 140 Arg Ile Pro Gly Gly Asp Gly Arg Val Asn Val Thr Ile Asn Cys Ser 145 150 155 160 Cys Gly Asp Glu Arg Val Ser Pro Arg Tyr Gly Leu Phe Leu Thr Tyr 165 170 175 Pro Leu Trp Asp Gly Glu Thr Leu Glu Ser Val Ala Ala Gln Tyr Gly 180 185 190 Phe Ser Ser Pro Ala Glu Met Glu Leu Ile Arg Arg Tyr Asn Pro Gly 195 200 205 Met Gly Gly Val Ser Gly Lys Gly Ile Val Phe Ile Pro Val Lys Asp 210 215 220 Pro Asn Gly Ser Tyr His Pro Leu Lys Ser Gly Val Gly Ile Val Leu 225 230 235 240 Leu Phe Cys Gly Met Gly Asn Ser Leu Ser Gly Gly Ala Ile Ala Gly 245 250 255 Ile Val Ile Ala Cys Ile Ala Ile Phe Ile Val Ala Ile Trp Leu Ile 260 265 270 Ile Met Phe Tyr Arg Trp Gln Lys Phe Arg Lys Ala Thr Ser Arg Pro 275 280 285 Ser Pro Glu Glu Thr Ser His Leu Asp Asp Ala Ser Gln Ala Glu Gly 290 295 300 Ile Lys Val Glu Arg Ser Ile Glu Phe Ser Tyr Glu Glu Ile Phe Asn 305 310 315 320 Ala Thr Gln Gly Phe Ser Met Glu His Lys Ile Gly Gln Gly Gly Phe 325 330 335 Gly Ser Val Tyr Tyr Ala Glu Leu Arg Gly Glu Lys Thr Ala Ile Lys 340 345 350 Lys Met Gly Met Gln Ala Thr Gln Glu Phe Leu Ala Glu Leu Lys Val 355 360 365 Leu Thr His Val His His Leu Asn Leu Val Arg Leu Ile Gly Tyr Cys 370 375 380 Val Glu Asn Cys Leu Phe Leu Val Tyr Glu Phe Ile Asp Asn Gly Asn 385 390 395 400 Leu Ser Gln His Leu Gln Arg Thr Gly Tyr Ala Pro Leu Ser Trp Ala 405 410 415 Thr Arg Val Gln Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu Tyr Leu 420 425 430 His Glu His Val Val Pro Val Tyr Val His Arg Asp Ile Lys Ser Ala 435 440 445 Asn Ile Leu Leu Asp Lys Asp Phe Arg Ala Lys Ile Ala Asp Phe Gly 450 455 460 Leu Ala Lys Leu Thr Glu Val Gly Ser Met Ser Gln Ser Leu Ser Thr 465 470 475 480 Arg Val Ala Gly Thr Phe Gly Tyr Met Pro Pro Glu Ala Arg Tyr Gly 485 490 495 Glu Val Ser Pro Lys Val Asp Val Tyr Ala Phe Gly Val Val Leu Tyr 500 505 510 Glu Leu Leu Ser Ala Lys Gln Ala Ile Val Arg Ser Ser Glu Ser Val 515 520 525 Ser Glu Ser Lys Gly Leu Val Phe Leu Phe Glu Glu Ala Leu Ser Ala 530 535 540 Pro Asn Pro Thr Glu Ala Leu Asp Glu Leu Ile Asp Pro Ser Leu Gln 545 550 555 560 Gly Asp Tyr Pro Val Asp Ser Ala Leu Lys Ile Ala Ser Leu Ala Lys 565 570 575 Ser Cys Thr His Glu Glu Pro Gly Met Arg Pro Thr Met Arg Ser Val 580 585 590 Val Val Ala Leu Met Ala Leu Thr Ala Asn Thr Asp Leu Arg Asp Met 595 600 605 Asp Tyr His Pro Phe 610 37 617 PRT 拟南芥 37 Met Lys Leu Lys Ile Ser Leu Ile Ala Pro Ile Leu Leu Leu Phe Ser 1 5 10 15 Phe Phe Phe Ala Val Glu Ser Lys Cys Arg Thr Ser Cys Pro Leu Ala 20 25 30 Leu Ala Ser Tyr Tyr Leu Glu Asn Gly Thr Thr Leu Ser Val Ile Asn 35 40 45 Gln Asn Leu Asn Ser Ser Ile Ala Pro Tyr Asp Gln Ile Asn Phe Asp 50 55 60 Pro Ile Leu Arg Tyr Asn Ser Asn Ile Lys Asp Lys Asp Arg Ile Gln 65 70 75 80 Met Gly Ser Arg Val Leu Val Pro Phe Pro Cys Glu Cys Gln Pro Gly 85 90 95 Asp Phe Leu Gly His Asn Phe Ser Tyr Ser Val Arg Gln Glu Asp Thr 100 105 110 Tyr Glu Arg Val Ala Ile Ser Asn Tyr Ala Asn Leu Thr Thr Met Glu 115 120 125 Ser Leu Gln Ala Arg Asn Pro Phe Pro Ala Thr Asn Ile Pro Leu Ser 130 135 140 Ala Thr Leu Asn Val Leu Val Asn Cys Ser Cys Gly Asp Glu Ser Val 145 150 155 160 Ser Lys Asp Phe Gly Leu Phe Val Thr Tyr Pro Leu Arg Pro Glu Asp 165 170 175 Ser Leu Ser Ser Ile Ala Arg Ser Ser Gly Val Ser Ala Asp Ile Leu 180 185 190 Gln Arg Tyr Asn Pro Gly Val Asn Phe Asn Ser Gly Asn Gly Ile Val 195 200 205 Tyr Val Pro Gly Arg Asp Pro Asn Gly Ala Phe Pro Pro Phe Lys Ser 210 215 220 Ser Lys Gln Asp Gly Val Gly Ala Gly Val Ile Ala Gly Ile Val Ile 225 230 235 240 Gly Val Ile Val Ala Leu Leu Leu Ile Leu Phe Ile Val Tyr Tyr Ala 245 250 255 Tyr Arg Lys Asn Lys Ser Lys Gly Asp Ser Phe Ser Ser Ser Ile Pro 260 265 270 Leu Ser Thr Lys Ala Asp His Ala Ser Ser Thr Ser Leu Gln Ser Gly 275 280 285 Gly Leu Gly Gly Ala Gly Val Ser Pro Gly Ile Ala Ala Ile Ser Val 290 295 300 Asp Lys Ser Val Glu Phe Ser Leu Glu Glu Leu Ala Lys Ala Thr Asp 305 310 315 320 Asn Phe Asn Leu Ser Phe Lys Ile Gly Gln Gly Gly Phe Gly Ala Val 325 330 335 Tyr Tyr Ala Glu Leu Arg Gly Glu Lys Ala Ala Ile Lys Lys Met Asp 340 345 350 Met Glu Ala Ser Lys Gln Phe Leu Ala Glu Leu Lys Val Leu Thr Arg 355 360 365 Val His His Val Asn Leu Val Arg Leu Ile Gly Tyr Cys Val Glu Gly 370 375 380 Ser Leu Phe Leu Val Tyr Glu Tyr Val Glu Asn Gly Asn Leu Gly Gln 385 390 395 400 His Leu His Gly Ser Gly Arg Glu Pro Leu Pro Trp Thr Lys Arg Val 405 410 415 Gln Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu Tyr Ile His Glu His 420 425 430 Thr Val Pro Val Tyr Val His Arg Asp Ile Lys Ser Ala Asn Ile Leu 435 440 445 Ile Asp Gln Lys Phe Arg Ala Lys Val Ala Asp Phe Gly Leu Thr Lys 450 455 460 Leu Thr Glu Val Gly Gly Ser Ala Thr Arg Gly Ala Met Gly Thr Phe 465 470 475 480 Gly Tyr Met Ala Pro Glu Thr Val Tyr Gly Glu Val Ser Ala Lys Val 485 490 495 Asp Val Tyr Ala Phe Gly Val Val Leu Tyr Glu Leu Ile Ser Ala Lys 500 505 510 Gly Ala Val Val Lys Met Thr Glu Ala Val Gly Glu Phe Arg Gly Leu 515 520 525 Val Gly Val Phe Glu Glu Ser Phe Lys Glu Thr Asp Lys Glu Glu Ala 530 535 540 Leu Arg Lys Ile Ile Asp Pro Arg Leu Gly Asp Ser Tyr Pro Phe Asp 545 550 555 560 Ser Val Tyr Lys Met Ala Glu Leu Gly Lys Ala Cys Thr Gln Glu Asn 565 570 575 Ala Gln Leu Arg Pro Ser Met Arg Tyr Ile Val Val Ala Leu Ser Thr 580 585 590 Leu Phe Ser Ser Thr Gly Asn Trp Asp Val Gly Asn Phe Gln Asn Glu 595 600 605 Asp Leu Val Ser Leu Met Ser Gly Arg 610 615 38 620 PRT 百脉根 38 Met Phe Tyr Asp Phe Thr Thr Met Ala Ser Leu Thr His Pro Leu Cys 1 5 10 15 Val Leu Leu Thr Leu Met Ala Ala Ala Ser Phe Ala Ser Val Phe Ser 20 25 30 Leu Glu Val Ser Ser Lys Thr Thr Tyr Met Glu Pro Phe Asn Cys Ser 35 40 45 Thr Lys Ile Arg Thr Cys Asn Ser Leu Leu Tyr His Ile Ser Ile Gly 50 55 60 Leu Lys Val Glu Glu Ile Ala Arg Phe Tyr Ser Val Asn Leu Ser Arg 65 70 75 80 Ile Lys Pro Ile Thr Arg Gly Thr Lys Gln Asp Tyr Leu Val Ser Val 85 90 95 Pro Cys Thr Cys Arg Asn Thr Asn Gly Leu Asn Gly Tyr Phe Tyr His 100 105 110 Thr Ser Tyr Lys Val Lys Val Asn Asp Ser Phe Val Asp Ile Gln Asn 115 120 125 Leu Phe Tyr Ser Gly Gln Ala Trp Pro Val Asn Glu Asp Leu Val Val 130 135 140 Pro Asn Glu Thr Met Thr Ile His Ile Pro Cys Gly Cys Ser Glu Ser 145 150 155 160 Gly Ser Gln Ile Val Val Thr Tyr Thr Val Gln Arg Asn Asp Thr Pro 165 170 175 Leu Ser Ile Ala Leu Leu Leu Asn Ala Thr Val Glu Gly Met Val Ser 180 185 190 Val Asn Ser Val Met Ala Pro Asn Pro Thr Phe Ile Asp Val Gly Trp 195 200 205 Val Leu Tyr Val Pro Lys Glu Leu Asn Pro Ile Ser His Gly Lys Glu 210 215 220 Asn Lys His Lys Leu Glu Lys Ile Ile Gly Ile Leu Ala Gly Val Ile 225 230 235 240 Leu Leu Ser Ile Ile Thr Leu Ile Ile Leu Ile Val Arg Arg Asn Arg 245 250 255 Ser Tyr Glu Thr Cys Lys Asp Asp Pro Arg Ala Ile Ser Lys Arg Ser 260 265 270 Ile Gly Lys Arg Thr Ser Ser Leu Met Asn Arg Asp Phe His Lys Glu 275 280 285 Tyr Met Glu Asp Ala Thr Ser Phe Asp Ser Glu Arg Pro Val Ile Tyr 290 295 300 Thr Leu Glu Glu Ile Glu Gln Ala Thr Asn Asp Phe Asp Glu Thr Arg 305 310 315 320 Arg Ile Gly Val Gly Gly Tyr Gly Thr Val Tyr Phe Gly Val Leu Gly 325 330 335 Glu Lys Glu Val Ala Ile Lys Lys Met Lys Ser Asn Lys Ser Lys Glu 340 345 350 Phe Tyr Ala Glu Leu Lys Ala Leu Cys Lys Ile His His Ile Asn Ile 355 360 365 Val Glu Leu Leu Gly Tyr Ala Ser Gly Asp Asp His Leu Tyr Leu Val 370 375 380 Tyr Glu Tyr Val Pro Asn Gly Ser Leu Ser Glu His Leu His Asp Pro 385 390 395 400 Leu Leu Lys Gly His Gln Pro Leu Ser Trp Cys Ala Arg Ile Gln Ile 405 410 415 Ala Leu Asp Ser Ala Lys Gly Ile Glu Tyr Ile His Asp Tyr Thr Lys 420 425 430 Ala Gln Tyr Val His Arg Asp Ile Lys Thr Ser Asn Ile Leu Leu Asp 435 440 445 Glu Lys Leu Arg Ala Lys Val Ala Asp Phe Gly Leu Ala Lys Leu Val 450 455 460 Glu Arg Thr Asn Asp Glu Glu Phe Ile Ala Thr Arg Leu Val Gly Thr 465 470 475 480 Pro Gly Tyr Leu Pro Pro Glu Ser Leu Lys Glu Leu Gln Val Thr Val 485 490 495 Lys Thr Asp Val Phe Ala Phe Gly Val Val Met Leu Glu Leu Ile Thr 500 505 510 Gly Lys Arg Ala Leu Phe Arg Asp Asn Gln Glu Ala Asn Asn Met Arg 515 520 525 Ser Leu Val Ala Val Val Asn Gln Ile Phe Gln Glu Asp Asn Pro Glu 530 535 540 Thr Ala Leu Glu Val Thr Val Asp Gly Asn Leu Gln Arg Ser Tyr Pro 545 550 555 560 Met Glu Asp Val Tyr Asn Met Ala Glu Leu Ser His Trp Cys Leu Arg 565 570 575 Glu Asn Pro Val Asp Arg Pro Glu Met Ser Glu Ile Val Val Lys Leu 580 585 590 Ser Lys Ile Ile Met Ser Ser Ile Glu Trp Glu Ala Ser Leu Gly Gly 595 600 605 Asp Ser Gln Val Phe Ser Gly Val Phe Asp Gly Arg 610 615 620 39 613 PRT 蒺藜苜蓿 39 Met Ala Ser Leu Ile Gln Leu Leu Ser Ile Phe Leu Pro Leu Leu Ala 1 5 10 15 Ser Ser Leu Pro Thr Ile Phe Ser Ile Glu Val Ser Met Lys Lys Ala 20 25 30 Tyr Met Glu Pro Tyr Lys Cys Ser Thr Lys Met Arg Thr Cys Asn Ala 35 40 45 Ser Leu Tyr His Ile Asn Tyr Asn His Asn Ile Glu Gln Ile Ala Asn 50 55 60 Phe Tyr Ser Ile Asp Pro Ser Gln Ile Lys Pro Ile Ile Arg Ser Thr 65 70 75 80 Lys Gln Asp Tyr Leu Val Lys Val Pro Cys Ser Cys Lys Asn Ile Lys 85 90 95 Asp Leu Ser Gly Tyr Phe Tyr Glu Thr Thr Tyr Lys Val Ser Pro Asn 100 105 110 Glu Thr Ser Val Asp Ile Met Asn Leu Ile Tyr Ser Gly Gln Ala Trp 115 120 125 Gln Val Asn Glu Asp Leu Val Ala Asn Glu Asn Val Thr Ile His Ile 130 135 140 Pro Cys Gly Cys Ser Glu Phe Glu Ser Gln Ile Val Val Thr Tyr Thr 145 150 155 160 Val Gln Gln Ser Asp Thr Pro Thr Ser Ile Ser Leu Leu Leu Asn Ala 165 170 175 Thr Ile Asp Gly Met Val Arg Ile Asn Gln Ile Leu Gly Pro Asn Pro 180 185 190 Thr Phe Ile Asp Ile Gly Trp Val Leu Tyr Val Pro Lys Glu Leu Lys 195 200 205 Gly Ser Pro Leu Tyr His Gly Lys Glu Lys Lys His Lys Trp Val Ile 210 215 220 Ile Ile Gly Ile Leu Val Ser Val Thr Leu Leu Ser Val Ile Thr Leu 225 230 235 240 Ile Ile Phe Ile Leu Arg Arg Asn Lys Ala Tyr Glu Thr Ser Lys Tyr 245 250 255 Asp Pro Lys Thr Val Ser Lys Arg Ser Phe Gly Asn Arg Thr Ile Ser 260 265 270 Leu Arg Asn His Glu Phe His Lys Glu Tyr Met Glu Asp Ala Thr Gln 275 280 285 Phe Asp Ser Glu Arg Pro Val Ile Tyr Asp Phe Glu Glu Ile Glu His 290 295 300 Ala Thr Asn Asn Phe Asp Glu Thr Arg Arg Ile Gly Val Gly Gly Tyr 305 310 315 320 Gly Thr Val Tyr Phe Gly Met Leu Glu Glu Lys Glu Val Ala Val Lys 325 330 335 Lys Met Lys Ser Asn Lys Ser Lys Glu Phe Tyr Ala Glu Leu Lys Ala 340 345 350 Leu Cys Lys Ile His His Ile Asn Ile Val Glu Leu Leu Gly Tyr Ala 355 360 365 Ser Gly Asp Asp His Leu Tyr Leu Val Tyr Glu Tyr Val Pro Asn Gly 370 375 380 Ser Leu Ser Glu His Leu His Asp Pro Leu Leu Lys Gly His Gln Pro 385 390 395 400 Leu Ser Trp Cys Ala Arg Thr Gln Ile Ala Leu Asp Ser Ala Lys Gly 405 410 415 Ile Glu Tyr Ile His Asp Tyr Thr Lys Ala Arg Tyr Val His Arg Asp 420 425 430 Ile Lys Thr Ser Asn Ile Leu Leu Asp Glu Lys Leu Arg Ala Lys Val 435 440 445 Ala Asp Phe Gly Leu Ala Lys Leu Val Glu Arg Thr Asn Asp Glu Glu 450 455 460 Phe Leu Ala Thr Arg Leu Val Gly Thr Pro Gly Tyr Leu Pro Pro Glu 465 470 475 480 Ser Val Lys Glu Leu Gln Val Thr Ile Lys Thr Asp Val Phe Ala Phe 485 490 495 Gly Val Val Ile Ser Glu Leu Ile Thr Gly Lys Arg Ala Leu Phe Arg 500 505 510 Asp Asn Lys Glu Ala Asn Asn Met Lys Ser Leu Ile Ala Val Val Asn 515 520 525 Lys Ile Phe Gln Asp Glu Asp Pro Val Ala Ala Leu Glu Ala Val Val 530 535 540 Asp Gly Asn Leu Leu Arg Asn Tyr Pro Ile Glu Gly Val Tyr Lys Met 545 550 555 560 Ala Glu Leu Ser His Trp Cys Leu Ser Glu Glu Pro Val Asp Arg Pro 565 570 575 Glu Met Lys Glu Ile Val Val Ala Val Ser Lys Ile Val Met Ser Ser 580 585 590 Ile Glu Trp Glu Ala Ser Leu Gly Gly Asp Ser Gln Val Phe Ser Gly 595 600 605 Val Phe Asp Gly Arg 610 40 595 PRT 百脉根 40 Met Ala Val Phe Phe Leu Thr Ser Gly Ser Leu Ser Leu Phe Leu Ala 1 5 10 15 Leu Thr Leu Leu Phe Thr Asn Ile Ala Ala Arg Ser Glu Lys Ile Ser 20 25 30 Gly Pro Asp Phe Ser Cys Pro Val Asp Ser Pro Pro Ser Cys Glu Thr 35 40 45 Tyr Val Thr Tyr Thr Ala Gln Ser Pro Asn Leu Leu Ser Leu Thr Asn 50 55 60 Ile Ser Asp Ile Phe Asp Ile Ser Pro Leu Ser Ile Ala Arg Ala Ser 65 70 75 80 Asn Ile Asp Ala Gly Lys Asp Lys Leu Val Pro Gly Gln Val Leu Leu 85 90 95 Val Pro Val Thr Cys Gly Cys Ala Gly Asn His Ser Ser Ala Asn Thr 100 105 110 Ser Tyr Gln Ile Gln Leu Gly Asp Ser Tyr Asp Phe Val Ala Thr Thr 115 120 125 Leu Tyr Glu Asn Leu Thr Asn Trp Asn Ile Val Gln Ala Ser Asn Pro 130 135 140 Gly Val Asn Pro Tyr Leu Leu Pro Glu Arg Val Lys Val Val Phe Pro 145 150 155 160 Leu Phe Cys Arg Cys Pro Ser Lys Asn Gln Leu Asn Lys Gly Ile Gln 165 170 175 Tyr Leu Ile Thr Tyr Val Trp Lys Pro Asn Asp Asn Val Ser Leu Val 180 185 190 Ser Ala Lys Phe Gly Ala Ser Pro Ala Asp Ile Leu Thr Glu Asn Arg 195 200 205 Tyr Gly Gln Asp Phe Thr Ala Ala Thr Asn Leu Pro Ile Leu Ile Pro 210 215 220 Val Thr Gln Leu Pro Glu Leu Thr Gln Pro Ser Ser Asn Gly Arg Lys 225 230 235 240 Ser Ser Ile His Leu Leu Val Ile Leu Gly Ile Thr Leu Gly Cys Thr 245 250 255 Leu Leu Thr Ala Val Leu Thr Gly Thr Leu Val Tyr Val Tyr Cys Arg 260 265 270 Arg Lys Lys Ala Leu Asn Arg Thr Ala Ser Ser Ala Glu Thr Ala Asp 275 280 285 Lys Leu Leu Ser Gly Val Ser Gly Tyr Val Ser Lys Pro Asn Val Tyr 290 295 300 Glu Ile Asp Glu Ile Met Glu Ala Thr Lys Asp Phe Ser Asp Glu Cys 305 310 315 320 Lys Val Gly Glu Ser Val Tyr Lys Ala Asn Ile Glu Gly Arg Val Val 325 330 335 Ala Val Lys Lys Ile Lys Glu Gly Gly Ala Asn Glu Glu Leu Lys Ile 340 345 350 Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Met Gly Val Ser 355 360 365 Ser Gly Tyr Asp Gly Asn Cys Phe Leu Val Tyr Glu Tyr Ala Glu Asn 370 375 380 Gly Ser Leu Ala Glu Trp Leu Phe Ser Lys Ser Ser Gly Thr Pro Asn 385 390 395 400 Ser Leu Thr Trp Ser Gln Arg Ile Ser Ile Ala Val Asp Val Ala Val 405 410 415 Gly Leu Gln Tyr Met His Glu His Thr Tyr Pro Arg Ile Ile His Arg 420 425 430 Asp Ile Thr Thr Ser Asn Ile Leu Leu Asp Ser Asn Phe Lys Ala Lys 435 440 445 Ile Ala Asn Phe Ala Met Ala Arg Thr Ser Thr Asn Pro Met Met Pro 450 455 460 Lys Ile Asp Val Phe Ala Phe Gly Val Leu Leu Ile Glu Leu Leu Thr 465 470 475 480 Gly Arg Lys Ala Met Thr Thr Lys Glu Asn Gly Glu Val Val Met Leu 485 490 495 Trp Lys Asp Met Trp Glu Ile Phe Asp Ile Glu Glu Asn Arg Glu Glu 500 505 510 Arg Ile Arg Lys Trp Met Asp Pro Asn Leu Glu Ser Phe Tyr His Ile 515 520 525 Asp Asn Ala Leu Ser Leu Ala Ser Leu Ala Val Asn Cys Thr Ala Asp 530 535 540 Lys Ser Leu Ser Arg Pro Ser Met Ala Glu Ile Val Leu Ser Leu Ser 545 550 555 560 Phe Leu Thr Gln Gln Ser Ser Asn Pro Thr Leu Glu Arg Ser Leu Thr 565 570 575 Ser Ser Gly Leu Asp Val Glu Asp Asp Ala His Ile Val Thr Ser Ile 580 585 590 Thr Ala Arg 595 41 632 PRT 蒺藜苜蓿 41 Met Lys Thr Leu Lys Pro His His His Gln Leu Ser Thr Phe Ile Phe 1 5 10 15 Ile Leu Leu Phe Pro Phe Leu Lys Ser Gln Thr Ala Arg Gln Gln Asn 20 25 30 Asn Thr Gly Tyr Thr Cys Pro Asn Asn Asn Asn Asn Asn Asn Asn Thr 35 40 45 Tyr Pro Cys Gln Thr Tyr Val Tyr Tyr Lys Ala Thr Pro Pro Asn Tyr 50 55 60 Leu Asp Leu Ala Thr Ile Ser Asp Leu Phe Gln Leu Ser Arg Leu Met 65 70 75 80 Ile Ser Lys Pro Ser Asn Ile Ser Ser Pro Ser Ser Pro Leu Leu Pro 85 90 95 Asn Gln Pro Leu Leu Ile Pro Leu Thr Cys Ser Cys Asn Phe Ile Asn 100 105 110 Thr Thr Phe Gly Ser Ile Ser Tyr Ser Asn Ile Thr Tyr Thr Ile Lys 115 120 125 Pro Asn Asp Thr Phe Phe Leu Val Ser Thr Ile Asn Phe Gln Asn Leu 130 135 140 Thr Thr Tyr Pro Ser Val Gln Val Val Asn Pro Asn Leu Val Ala Thr 145 150 155 160 Asn Leu Ser Ile Gly Asp Asn Ala Val Phe Pro Ile Phe Cys Lys Cys 165 170 175 Pro Asp Lys Thr Lys Thr Asn Ser Ser Phe Met Ile Ser Tyr Val Val 180 185 190 Gln Pro His Asp Asn Val Ser Ser Ile Ala Ser Met Phe Gly Thr Ser 195 200 205 Glu Lys Ser Ile Val Asp Val Asn Gly Glu Arg Leu Tyr Asp Tyr Asp 210 215 220 Thr Ile Phe Val Pro Val Thr Glu Leu Pro Val Leu Lys Gln Pro Ser 225 230 235 240 Thr Ile Val Pro Ser Pro Ala Pro Arg Gly Asn Ser Asp Asp Gly Asp 245 250 255 Asp Asp Asp Asp Lys Ser Gly Ile Val Lys Gly Leu Ala Ile Gly Leu 260 265 270 Gly Ile Leu Gly Phe Leu Leu Ile Leu Val Ile Val Phe Trp Phe Tyr 275 280 285 Arg Glu Val Leu Phe Lys Lys Glu Lys Lys Gly Lys Gly Leu Tyr Phe 290 295 300 Gly Asp Lys Gly Tyr Lys Gly Asn Asp Glu Lys Lys Lys Lys Met Asp 305 310 315 320 Val Asn Phe Met Ala Asn Val Ser Asp Cys Leu Asp Lys Tyr Arg Val 325 330 335 Phe Gly Phe Asp Glu Leu Val Glu Ala Thr Asp Gly Phe Asp Glu Arg 340 345 350 Phe Leu Ile Gln Gly Ser Val Tyr Lys Gly Glu Ile Asp Gly Gln Val 355 360 365 Tyr Ala Ile Lys Lys Met Lys Trp Asn Ala Tyr Glu Glu Leu Lys Ile 370 375 380 Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Glu Gly Phe Cys 385 390 395 400 Ile Glu Pro Glu Glu Ser Asn Cys Tyr Leu Val Tyr Glu Tyr Val Glu 405 410 415 Asn Gly Ser Leu Tyr Ser Trp Leu His Glu Asp Lys Asn Glu Lys Leu 420 425 430 Asn Trp Val Thr Arg Leu Arg Ile Ala Val Asp Ile Ala Asn Gly Leu 435 440 445 Leu Tyr Ile His Glu His Thr Arg Pro Lys Val Val His Lys Asp Ile 450 455 460 Lys Ser Ser Asn Ile Leu Leu Asp Ser Asn Met Arg Ala Lys Ile Ala 465 470 475 480 Asn Phe Gly Leu Ala Lys Ser Gly Ile Asn Ala Ile Thr Met His Ile 485 490 495 Val Gly Thr Gln Gly Tyr Ile Ser Pro Glu Tyr Leu Ala Asp Gly Ile 500 505 510 Val Ser Thr Lys Met Asp Val Phe Ser Phe Gly Ile Val Leu Leu Glu 515 520 525 Leu Ile Ser Gly Lys Glu Val Ile Asp Glu Glu Gly Asn Val Leu Trp 530 535 540 Ala Ser Ala Ile Lys Thr Phe Glu Val Lys Asn Glu Gln Glu Lys Ala 545 550 555 560 Arg Arg Leu Lys Glu Trp Leu Asp Arg Thr Met Leu Lys Glu Thr Cys 565 570 575 Ser Met Glu Ser Leu Met Gly Val Leu His Val Ala Ile Ala Cys Leu 580 585 590 Asn Arg Asp Pro Ser Lys Arg Pro Ser Ile Ile Asp Ile Val Tyr Ser 595 600 605 Leu Ser Lys Cys Glu Glu Ala Gly Phe Glu Leu Ser Asp Asp Gly Phe 610 615 620 Gly Ser Glu Arg Leu Val Ala Arg 625 630 42 423 PRT 拟南芥 42 Met Lys Asn Pro Glu Lys Pro Leu Leu Leu Phe Leu Ile Leu Ala Ser 1 5 10 15 Ser Leu Ala Ser Met Ala Thr Ala Lys Ser Thr Ile Glu Pro Cys Ser 20 25 30 Ser Lys Asp Thr Cys Asn Ser Leu Leu Gly Tyr Thr Leu Tyr Thr Asp 35 40 45 Leu Lys Val Thr Glu Val Ala Ser Leu Phe Gln Val Asp Pro Val Ser 50 55 60 Met Leu Leu Ser Asn Ser Ile Asp Ile Ser Tyr Pro Asp Val Glu Asn 65 70 75 80 His Val Leu Pro Ala Lys Leu Phe Leu Lys Ile Pro Ile Thr Cys Ser 85 90 95 Cys Val Asp Gly Ile Arg Lys Ser Leu Ser Thr His Tyr Lys Thr Arg 100 105 110 Thr Ser Asp Thr Leu Gly Ser Ile Ala Asp Ser Val Tyr Gly Gly Leu 115 120 125 Val Ser Pro Glu Gln Ile Gln Val Ala Asn Ser Glu Thr Asp Leu Ser 130 135 140 Val Leu Asp Val Gly Thr Lys Leu Val Ile Pro Leu Pro Cys Ala Cys 145 150 155 160 Phe Asn Gly Thr Asp Glu Ser Leu Pro Ala Leu Tyr Leu Ser Tyr Val 165 170 175 Val Arg Gly Ile Asp Thr Met Ala Gly Ile Ala Lys Arg Phe Ser Thr 180 185 190 Ser Val Thr Asp Leu Thr Asn Val Asn Ala Met Gly Ala Pro Asp Ile 195 200 205 Asn Pro Gly Asp Ile Leu Ala Val Pro Leu Leu Ala Cys Ser Ser Asn 210 215 220 Phe Pro Lys Tyr Ala Thr Asp Tyr Gly Leu Ile Ile Pro Asn Gly Ser 225 230 235 240 Tyr Ala Leu Thr Ala Gly His Cys Val Gln Cys Ser Cys Val Leu Gly 245 250 255 Ser Arg Ser Met Tyr Cys Glu Pro Ala Ser Ile Ser Val Ser Cys Ser 260 265 270 Ser Met Arg Cys Arg Asn Ser Asn Phe Met Leu Gly Asn Ile Thr Ser 275 280 285 Gln Gln Ser Ser Ser Gly Cys Lys Leu Thr Thr Cys Ser Tyr Asn Gly 290 295 300 Phe Ala Ser Gly Thr Ile Leu Thr Thr Leu Ser Met Ser Leu Gln Pro 305 310 315 320 Arg Cys Pro Gly Pro Gln Gln Leu Ala Pro Leu Ile Ala Pro Pro Asp 325 330 335 Asn Val Pro Lys Glu Leu Met Tyr Leu Pro Ser Pro Ser Pro Ser Pro 340 345 350 Ser Pro Glu Phe Asp Asp Ile Ala Gly Gly Gly Ser Ser Ile Ala Ala 355 360 365 Val Pro Ala Ala Ser Pro Gly Gly Ala Thr Val Ser Ser Ser Asn Ser 370 375 380 Ile Pro Gly Asn Pro Ala Asn Gly Pro Gly Gly Ser Ile Ser Ile Ala 385 390 395 400 Ser Cys Pro Leu Ser Tyr Tyr Ser Phe Ile Ala Leu Leu Ile Pro Ile 405 410 415 Gly Ser Cys Phe Phe Val Phe 420 43 382 PRT 粳稻组 43 Pro Ala Pro Val Ser Thr Arg Gln Ser Asn Lys His Gln Ala Ser His 1 5 10 15 Ser His Leu Ser Arg Arg Ala Phe Pro Thr Met Ala Ser Leu Thr Ala 20 25 30 Ala Leu Ala Thr Pro Ala Ala Ala Ala Leu Leu Leu Leu Val Leu Leu 35 40 45 Ala Ala Pro Ala Ser Ala Ala Asn Phe Thr Cys Ala Val Ala Ser Gly 50 55 60 Thr Thr Cys Lys Ser Ala Ile Leu Tyr Thr Ser Pro Asn Ala Thr Thr 65 70 75 80 Tyr Gly Asn Leu Val Ala Arg Phe Asn Thr Thr Thr Leu Pro Asp Leu 85 90 95 Leu Gly Ala Asn Gly Leu Pro Asp Gly Thr Leu Ser Ser Ala Pro Val 100 105 110 Ala Ala Asn Ser Thr Val Lys Ile Pro Phe Arg Cys Arg Cys Asn Gly 115 120 125 Asp Val Gly Gln Ser Asp Arg Leu Pro Ile Tyr Val Val Gln Pro Gln 130 135 140 Asp Gly Leu Asp Ala Ile Ala Arg Asn Val Phe Asn Ala Phe Val Thr 145 150 155 160 Tyr Gln Glu Ile Ala Ala Ala Asn Asn Ile Pro Asp Pro Asn Lys Ile 165 170 175 Asn Val Ser Gln Thr Leu Trp Ile Pro Leu Pro Cys Ser Cys Asp Lys 180 185 190 Glu Glu Gly Ser Asn Val Met His Leu Ala Tyr Ser Val Gly Lys Gly 195 200 205 Glu Asn Thr Ser Ala Ile Ala Ala Lys Tyr Gly Val Thr Glu Ser Thr 210 215 220 Leu Leu Thr Arg Asn Lys Ile Asp Asp Pro Thr Lys Leu Gln Met Gly 225 230 235 240 Gln Ile Leu Asp Val Pro Leu Pro Val Cys Arg Ser Ser Ile Ser Asp 245 250 255 Thr Ser Ala Asp His Asn Leu Met Leu Leu Pro Asp Gly Thr Tyr Gly 260 265 270 Phe Thr Ala Gly Asn Cys Ile Arg Cys Ser Cys Ser Ser Thr Thr Tyr 275 280 285 Gln Leu Asn Cys Thr Ala Val Gln Asn Lys Gly Cys Pro Ser Val Pro 290 295 300 Leu Cys Asn Gly Thr Leu Lys Leu Gly Glu Thr Asn Gly Thr Gly Cys 305 310 315 320 Gly Ser Thr Thr Cys Ala Tyr Ser Gly Tyr Ser Asn Ser Ser Ser Leu 325 330 335 Ile Ile Gln Thr Ser Leu Ala Thr Asn Gln Thr Thr Ala Cys Gln Arg 340 345 350 Gly Gly Ser Gly Arg Ser Gln Phe Ala Arg Ser Met Trp Ser Met Ser 355 360 365 Val Ile Ser Phe His Met Val Leu Ile Ile Ile Cys Phe Leu 370 375 380 44 350 PRT 拟南芥 44 Met Glu Thr Ser Cys Phe Thr Leu Leu Gly Leu Leu Val Ser Leu Ser 1 5 10 15 Phe Phe Leu Thr Leu Ser Ala Gln Met Thr Gly Asn Phe Asn Cys Ser 20 25 30 Gly Ser Thr Ser Thr Cys Gln Ser Leu Val Gly Tyr Ser Ser Lys Asn 35 40 45 Ala Thr Thr Leu Arg Asn Ile Gln Thr Leu Phe Ala Val Lys Asn Leu 50 55 60 Arg Ser Ile Leu Gly Ala Asn Asn Leu Pro Leu Asn Thr Ser Arg Asp 65 70 75 80 Gln Arg Val Asn Pro Asn Gln Val Val Arg Val Pro Ile His Cys Ser 85 90 95 Cys Ser Asn Gly Thr Gly Val Ser Asn Arg Asp Ile Glu Tyr Thr Ile 100 105 110 Lys Lys Asp Asp Ile Leu Ser Phe Val Ala Thr Glu Ile Phe Gly Gly 115 120 125 Leu Val Thr Tyr Glu Lys Ile Ser Glu Val Asn Lys Ile Pro Asp Pro 130 135 140 Asn Lys Ile Glu Ile Gly Gln Lys Phe Trp Ile Pro Leu Pro Cys Ser 145 150 155 160 Cys Asp Lys Leu Asn Gly Glu Asp Val Val His Tyr Ala His Val Val 165 170 175 Lys Leu Gly Ser Ser Leu Gly Glu Ile Ala Ala Gln Phe Gly Thr Asp 180 185 190 Asn Thr Thr Leu Ala Gln Leu Asn Gly Ile Ile Gly Asp Ser Gln Leu 195 200 205 Leu Ala Asp Lys Pro Leu Asp Val Pro Leu Lys Ala Cys Ser Ser Ser 210 215 220 Val Arg Lys Asp Ser Leu Asp Ala Pro Leu Leu Leu Ser Asn Asn Ser 225 230 235 240 Tyr Val Phe Thr Ala Asn Asn Cys Val Lys Cys Thr Cys Asp Ala Leu 245 250 255 Lys Asn Trp Thr Leu Ser Cys Gln Ser Ser Ser Glu Ile Lys Pro Ser 260 265 270 Asn Trp Gln Thr Cys Pro Pro Phe Ser Gln Cys Asp Gly Ala Leu Leu 275 280 285 Asn Ala Ser Cys Arg Gln Pro Arg Asp Cys Val Tyr Ala Gly Tyr Ser 290 295 300 Asn Gln Thr Ile Phe Thr Thr Ala Ser Pro Ala Cys Pro Asp Ser Ala 305 310 315 320 Gly Pro Asp Asn Tyr Ala Ser Thr Leu Ser Ser Ser Phe Asn Phe Val 325 330 335 Ile Val Leu Ile Gln Cys Ala Leu Leu Cys Leu Cys Leu Leu 340 345 350 45 632 PRT 蒺藜苜蓿 45 Met Lys Thr Leu Lys Pro His His His Gln Leu Ser Thr Phe Ile Phe 1 5 10 15 Ile Leu Leu Phe Pro Phe Leu Lys Ser Gln Thr Ala Arg Gln Gln Asn 20 25 30 Asn Thr Gly Tyr Thr Cys Pro Asn Asn Asn Asn Asn Asn Asn Asn Thr 35 40 45 Tyr Pro Cys Gln Thr Tyr Val Tyr Tyr Lys Ala Thr Pro Pro Asn Tyr 50 55 60 Leu Asp Leu Ala Thr Ile Ser Asp Leu Phe Gln Leu Ser Arg Leu Met 65 70 75 80 Ile Ser Lys Pro Ser Asn Ile Ser Ser Pro Ser Ser Pro Leu Leu Pro 85 90 95 Asn Gln Pro Leu Leu Ile Pro Leu Thr Cys Ser Cys Asn Phe Ile Asn 100 105 110 Thr Thr Phe Gly Ser Ile Ser Tyr Ser Asn Ile Thr Tyr Thr Ile Lys 115 120 125 Pro Asn Asp Thr Phe Phe Leu Val Ser Thr Ile Asn Phe Gln Asn Leu 130 135 140 Thr Thr Tyr Pro Ser Val Gln Val Val Asn Pro Asn Leu Val Ala Thr 145 150 155 160 Asn Leu Ser Ile Gly Asp Asn Ala Val Phe Pro Ile Phe Cys Lys Cys 165 170 175 Pro Asp Lys Thr Lys Thr Asn Ser Ser Phe Met Ile Ser Tyr Val Val 180 185 190 Gln Pro His Asp Asn Val Ser Ser Ile Ala Ser Met Phe Gly Thr Ser 195 200 205 Glu Lys Ser Ile Val Asp Val Asn Gly Glu Arg Leu Tyr Asp Tyr Asp 210 215 220 Thr Ile Phe Val Pro Val Thr Glu Leu Pro Val Leu Lys Gln Pro Ser 225 230 235 240 Thr Ile Val Pro Ser Pro Ala Pro Arg Gly Asn Ser Asp Asp Gly Asp 245 250 255 Asp Asp Asp Asp Lys Ser Gly Ile Val Lys Gly Leu Ala Ile Gly Leu 260 265 270 Gly Ile Leu Gly Phe Leu Leu Ile Leu Val Ile Val Phe Trp Phe Tyr 275 280 285 Arg Glu Val Leu Phe Lys Lys Glu Lys Lys Gly Lys Gly Leu Tyr Phe 290 295 300 Gly Asp Lys Gly Tyr Lys Gly Asn Asp Glu Lys Lys Lys Lys Met Asp 305 310 315 320 Val Asn Phe Met Ala Asn Val Ser Asp Cys Leu Asp Lys Tyr Arg Val 325 330 335 Phe Gly Phe Asp Glu Leu Val Glu Ala Thr Asp Gly Phe Asp Glu Arg 340 345 350 Phe Leu Ile Gln Gly Ser Val Tyr Lys Gly Glu Ile Asp Gly Gln Val 355 360 365 Tyr Ala Ile Lys Lys Met Lys Trp Asn Ala Tyr Glu Glu Leu Lys Ile 370 375 380 Leu Gln Lys Val Asn His Gly Asn Leu Val Lys Leu Glu Gly Phe Cys 385 390 395 400 Ile Glu Pro Glu Glu Ser Asn Cys Tyr Leu Val Tyr Glu Tyr Val Glu 405 410 415 Asn Gly Ser Leu Tyr Ser Trp Leu His Glu Asp Lys Asn Glu Lys Leu 420 425 430 Asn Trp Val Thr Arg Leu Arg Ile Ala Val Asp Ile Ala Asn Gly Leu 435 440 445 Leu Tyr Ile His Glu His Thr Arg Pro Lys Val Val His Lys Asp Ile 450 455 460 Lys Ser Ser Asn Ile Leu Leu Asp Ser Asn Met Arg Ala Lys Ile Ala 465 470 475 480 Asn Phe Gly Leu Ala Lys Ser Gly Ile Asn Ala Ile Thr Met His Ile 485 490 495 Val Gly Thr Gln Gly Tyr Ile Ser Pro Glu Tyr Leu Ala Asp Gly Ile 500 505 510 Val Ser Thr Lys Met Asp Val Phe Ser Phe Gly Ile Val Leu Leu Glu 515 520 525 Leu Ile Ser Gly Lys Glu Val Ile Asp Glu Glu Gly Asn Val Leu Trp 530 535 540 Ala Ser Ala Ile Lys Thr Phe Glu Val Lys Asn Glu Gln Glu Lys Ala 545 550 555 560 Arg Arg Leu Lys Glu Trp Leu Asp Arg Thr Met Leu Lys Glu Thr Cys 565 570 575 Ser Met Glu Ser Leu Met Gly Val Leu His Val Ala Ile Ala Cys Leu 580 585 590 Asn Arg Asp Pro Ser Lys Arg Pro Ser Ile Ile Asp Ile Val Tyr Ser 595 600 605 Leu Ser Lys Cys Glu Glu Ala Gly Phe Glu Leu Ser Asp Asp Gly Phe 610 615 620 Gly Ser Glu Arg Leu Val Ala Arg 625 630 46 647 PRT 玉米 46 Met Arg Pro Pro Ala Leu Arg Leu Ser Ala Leu Leu Leu Phe Leu Cys 1 5 10 15 Leu Tyr Ala Ala Pro Ala Arg Ser Gln Asn Ala Thr Thr Val Thr Ala 20 25 30 Ala Pro Ala Ser Val Glu Gly Phe Asn Cys Ser Val Asn Arg Thr Tyr 35 40 45 Pro Cys Gln Ala Tyr Ala Leu Tyr Arg Ala Gly Phe Ala Gly Val Pro 50 55 60 Leu Asn Leu Ala Ala Ile Gly Asp Leu Phe Ala Ala Ser Arg Phe Met 65 70 75 80 Val Ala His Ala Asn Asn Leu Ser Thr Ala Ala Ala Pro Ala Thr Gly 85 90 95 Gln Pro Leu Leu Val Pro Leu Gln Cys Gly Cys Pro Ser Gly Ser Pro 100 105 110 Asn Ser Tyr Ala Pro Met Gln Tyr Gln Ile Ala Ser Gly Asp Thr Tyr 115 120 125 Trp Ile Ile Ser Thr Thr Lys Leu Gln Asn Leu Thr Gln Tyr Gln Ala 130 135 140 Val Glu Arg Val Asn Pro Thr Leu Val Pro Thr Asn Leu Asp Val Gly 145 150 155 160 Thr Met Val Thr Phe Pro Ile Phe Cys Gln Cys Pro Ala Ala Ala Asp 165 170 175 Asn Ala Thr Ala Leu Val Thr Tyr Val Met Gln Pro Gly Asp Thr Tyr 180 185 190 Ser Thr Ile Ala Ala Ala Phe Ser Val Asp Ala Gln Ser Leu Val Ser 195 200 205 Leu Asn Gly Pro Glu Pro Arg Thr Gln Gln Phe Ala Glu Ile Leu Val 210 215 220 Pro Leu Arg Arg Gln Val Pro Gly Trp Leu Pro Pro Ile Val Leu Arg 225 230 235 240 Asn Asn Ala Ser Ala Thr Pro Ala Ala Pro Pro Pro Ser Ala Ser Pro 245 250 255 Asn Ala Thr Val Val Arg Asn Asp Arg Asn Gly Val Val Thr Gly Leu 260 265 270 Ala Val Gly Leu Gly Val Val Gly Ala Leu Trp Leu Leu Gln Met Leu 275 280 285 Leu Leu Ala Cys Leu Cys Arg Arg Leu Arg Ala Asn Gly Arg Arg Gly 290 295 300 Asp Ala Val Leu Ser Gly Asp Gly Val Glu Gly Gly Val Phe Ala Lys 305 310 315 320 Gly Ser Ser Ala Ala Ala Ala Gly Gly Gly Glu Arg Phe Leu Val Ser 325 330 335 Asp Met Ser Glu Trp Leu Asp Lys Tyr Arg Val Phe Thr Val Glu Glu 340 345 350 Leu Glu Arg Gly Thr Gly Gly Phe Asp Asp Ala His Leu Val Asn Gly 355 360 365 Ser Val Tyr Lys Ala Asn Ile Asp Gly Leu Val Phe Ala Val Lys Lys 370 375 380 Met Lys Trp Asp Ala Cys Glu Glu Leu Lys Ile Leu Gln Lys Val Asn 385 390 395 400 His Ser Asn Leu Val Lys Leu Glu Gly Phe Cys Ile Asp Ser Ala Thr 405 410 415 Gly Asp Cys Tyr Leu Val Tyr Glu Tyr Val Glu Asn Gly Ser Leu Asp 420 425 430 Leu Trp Leu Leu Asp Arg Asp His Ala Arg Arg Leu Asn Trp Arg Ala 435 440 445 Arg Leu His Ile Ala Leu Asp Leu Ala His Gly Leu Gln Tyr Ile His 450 455 460 Glu His Thr Trp Pro Arg Val Val His Lys Asp Met Lys Ser Ser Asn 465 470 475 480 Val Leu Leu Asp Ala Arg Met Arg Ala Lys Ile Ala Asn Phe Gly Leu 485 490 495 Ala Lys Thr Gly His Asn Ala Ile Thr Thr His Ile Val Gly Thr Gln 500 505 510 Gly Tyr Ile Ala Pro Glu Tyr Leu Ala Asp Gly Leu Val Thr Thr Lys 515 520 525 Ile Asp Val Phe Ala Tyr Gly Val Val Leu Leu Glu Leu Val Ser Gly 530 535 540 Arg Glu Ala Ala Asp Glu Ser Gly Glu Pro Leu Trp Ala Asp Ala Glu 545 550 555 560 Asp Arg Val Phe Arg Gly Arg Asp Glu Arg Leu Glu Ala Arg Val Ala 565 570 575 Ala Trp Met Asp Pro Ala Leu Ala Glu Gln Thr Cys Pro Leu Gly Ser 580 585 590 Val Ala Thr Val Val Ser Val Ala Arg Ala Cys Leu His Lys Asp Pro 595 600 605 Ser Lys Arg Pro Ser Met Val Asp Val Ala Tyr Thr Leu Ser Lys Ala 610 615 620 Asp Glu His Phe Ala Asp Tyr Ser Gly Glu Ser Val Ser Val Asp Gly 625 630 635 640 Ser Gly Glu Ile Ala Ala Arg 645 47 680 PRT 玉米 47 Met Ala Pro Pro Pro Gln Pro Glu Leu Pro Ala Ala Ala Leu Ala Leu 1 5 10 15 Leu Val Leu Leu Leu Ala Ala Ala Val Pro Ala Arg Ala Gln Gln Glu 20 25 30 Tyr Glu Ala Asn Lys Gln Asn Ala Cys Tyr Ala Thr Asn Ala Ser Ser 35 40 45 Val Leu Gly Tyr Thr Cys Asn Ala Thr Ala Ala Ser Thr Pro Ala Cys 50 55 60 Glu Ser Tyr Leu Ile Phe Arg Ser Ser Pro Ser Tyr Tyr Asn Thr Pro 65 70 75 80 Val Ser Ile Ser Tyr Leu Leu Asn Ser Ser Pro Ala Thr Val Ala Ala 85 90 95 Ala Asn Ala Val Pro Thr Val Ser Pro Leu Ala Ala Ser Ser Leu Val 100 105 110 Leu Val Pro Val Pro Cys Ala Cys Thr Pro Gly Gly Tyr Tyr Gln His 115 120 125 Asn Ser Ser Tyr Thr Ile Glu Phe Gln Ser Glu Thr Tyr Phe Ile Ile 130 135 140 Ala Asn Ile Thr Tyr Gln Gly Leu Thr Thr Cys Gln Ala Leu Ile Ala 145 150 155 160 Gln Asn Pro Leu His Asp Ser Arg Gly Leu Val Ala Gly Asn Asn Leu 165 170 175 Thr Val Pro Leu Arg Cys Ala Cys Pro Ser Pro Ala Gln Ala Ala Lys 180 185 190 Gly Phe Arg Tyr Leu Leu Ser Tyr Leu Val Met Trp Gly Asp Gly Val 195 200 205 Pro Ser Ile Ala Ala Arg Phe Arg Val Asp Pro Gln Ala Val Leu Asp 210 215 220 Ala Asn Ser Leu Thr Ala Asp Asp Ile Ile Phe Pro Phe Thr Thr Leu 225 230 235 240 Leu Ile Pro Leu Lys Ala Ala Pro Thr Pro Asp Met Leu Ala Ser Pro 245 250 255 Ala Pro Pro Pro Ser Pro Thr Pro Pro Gln Pro Thr Pro Ala Pro Ser 260 265 270 Gly Gly Ser Gly Ser Gly Lys Trp Val Gly Val Gly Val Gly Leu Gly 275 280 285 Cys Gly Ala Leu Ala Leu Ala Ala Ile Leu Gly Leu Leu Phe Leu Arg 290 295 300 Thr Arg Arg Arg Arg Gly Gln Arg Phe Ala Asp Gly Glu Ser Val Arg 305 310 315 320 Gln Gly Ser Lys Val Val Ile Asp Val Ser Ser Ser Ala Glu Tyr Gly 325 330 335 Ala Leu Ala Ser Gly Lys Gln Thr Ser Asn Thr Thr Thr Ser Thr Thr 340 345 350 Ser Ser Ala Thr Arg Ser Leu Val Ala Ser Asp Val Arg Gly Ala Val 355 360 365 Glu Ser Leu Thr Val Tyr Lys Tyr Ser Glu Leu Glu Lys Ala Thr Ala 370 375 380 Gly Phe Ala Glu Glu Arg Gln Val Pro Gly Thr Ser Val Phe Arg Ala 385 390 395 400 Val Ile Asn Gly Asp Ala Ala Ala Val Lys Leu Val Ala Gly Asp Val 405 410 415 Arg Asp Glu Val Ser Ile Leu Met Arg Val Asn His Ser Cys Leu Val 420 425 430 Arg Leu Ser Gly Leu Cys Val His Arg Gly Asp Thr Tyr Leu Val Phe 435 440 445 Glu Phe Ala Glu Asn Gly Ala Leu Ser Asp Trp Ile His Gly Gly Gly 450 455 460 Gly Ser Thr Leu Arg Trp Arg Gln Arg Val Gln Val Ala Phe Asp Val 465 470 475 480 Ala Asp Gly Leu Asn Tyr Leu His His Tyr Thr Asn Pro Pro Cys Val 485 490 495 His Lys Asn Leu Lys Ser Ser Asn Val Leu Leu Asp Ala Asp Leu Arg 500 505 510 Ala Lys Val Ser Ser Phe Gly Leu Ala Arg Thr Val Ala Ala Ser Asp 515 520 525 Gly Gly Ala Gln Leu Thr Arg His Val Ala Gly Thr Gln Gly Tyr Leu 530 535 540 Ala Pro Glu Tyr Leu Glu Asp Gly Leu Ile Thr Pro Lys Leu Asp Val 545 550 555 560 Phe Ala Phe Gly Val Val Leu Leu Glu Leu Leu Ser Gly Lys Glu Ala 565 570 575 Ala Phe Ala Asp Ala Gly Thr Gly Glu Glu Thr Leu Leu Trp Glu Ala 580 585 590 Ala Glu Glu Ala Leu Val Ala Asp Gly Gly Glu Asp Val Asp Arg Ala 595 600 605 Lys Val Arg Ala Phe Met Asp Pro Arg Leu His Gly Asp Phe Pro Ile 610 615 620 Asp Leu Ala Leu Ala Met Ala Ala Leu Ala Leu Arg Cys Val Ala Thr 625 630 635 640 Glu Pro Arg Ala Arg Pro Ala Met Asp Glu Val Phe Val Ser Leu Thr 645 650 655 Ala Val His Asn Ser Thr Leu Asp Trp Asp Pro Ser Asp Tyr Gly Thr 660 665 670 Ser Gly Ser Ser Met Val Gly Arg 675 680 48 684 PRT 玉米 48 Met Met Ala Ser Pro Pro Gln Thr Glu Leu Gln Ala Val Ala Leu Ala 1 5 10 15 Leu Leu Val Leu Leu Leu Ala Gly Ala Ala Pro Ala Arg Ala Gln Gln 20 25 30 Glu Tyr Glu Ala Asn Lys Gln Asn Ala Cys Tyr Ala Thr Asn Ala Ser 35 40 45 Ser Val Leu Gly Tyr Thr Cys Asn Ala Thr Thr Ala Ser Thr Pro Ala 50 55 60 Cys Asp Ser Tyr Leu Ile Phe Arg Ser Ser Pro Thr Tyr Tyr Asn Thr 65 70 75 80 Pro Val Ser Ile Ser Tyr Leu Leu Asn Ser Ser Val Ser Ala Thr Ala 85 90 95 Ala Ala Asn Ala Val Pro Ser Val Ser Pro Leu Ala Pro Ser Ser Leu 100 105 110 Val Leu Val Pro Val Pro Cys Ala Cys Thr Pro Gly Gly Tyr Tyr Gln 115 120 125 His Asn Ser Ser Tyr Thr Ile Gln Phe Arg Gly Glu Thr Tyr Phe Ile 130 135 140 Ile Ala Asn Ile Thr Tyr Gln Gly Leu Thr Thr Cys Gln Ala Leu Ile 145 150 155 160 Ala His Asn Pro Leu His Asp Ser Arg Gly Leu Val Ala Gly Asn Asn 165 170 175 Leu Thr Val Pro Leu Arg Cys Ala Cys Pro Ser Pro Ala Gln Ala Ala 180 185 190 Lys Gly Phe Lys Tyr Leu Leu Ser Tyr Leu Ile Met Trp Gly Asp Asp 195 200 205 Val Thr Ser Ile Ala Ala Arg Phe Arg Ala Asp Pro Gln Ala Val Leu 210 215 220 Asp Ala Asn Ser Leu Thr Ala Asp Asp Ile Ile Phe Pro Phe Thr Thr 225 230 235 240 Leu Leu Ile Pro Leu Lys Thr Ala Pro Thr Leu Asp Met Leu Ala Ser 245 250 255 Thr Ala Pro Pro Pro Ala Pro Thr Pro Pro Gln Pro Ala Pro Ala Pro 260 265 270 Ser Gly Arg Ser Gly Ser Gly Lys Leu Val Gly Phe Gly Val Gly Leu 275 280 285 Gly Cys Gly Ala Leu Ala Leu Ala Gly Ile Leu Gly Leu Leu Phe Leu 290 295 300 Arg Ala Arg Arg Arg Gln Arg Leu Pro Val Gly Glu Ser Val Arg Gln 305 310 315 320 Gly Ser Lys Val Val Ile Asp Val Ser Ser Ser Ala Asp Tyr Gly Ala 325 330 335 Leu Ala Ser Gly Lys Lys Ile Thr Asn Thr Thr Thr Ser Ser Met Ser 340 345 350 Ser Ala Ala Trp Ser Leu Val Ala Ser Asp Val Arg Gly Ala Val Glu 355 360 365 Ser Leu Thr Val Tyr Lys Tyr Ser Glu Leu Glu Lys Ala Thr Ala Gly 370 375 380 Phe Ala Glu Glu His Gln Val Pro Gly Thr Ser Val Tyr Arg Ala Val 385 390 395 400 Ile Asn Gly Asp Ala Ala Ala Val Lys Arg Leu Ala Gly Asp Val Ser 405 410 415 Gly Glu Val Gly Ile Leu Met Arg Val Asn His Ser Cys Leu Val Arg 420 425 430 Leu Ser Gly Leu Cys Val His Arg Gly Asp Thr Tyr Leu Val Phe Glu 435 440 445 Phe Ala Glu Asn Gly Ala Leu Ser Asp Trp Ile His Gly Gly Ser Gly 450 455 460 Ser Cys Ser Gly Ser Asn Thr Leu Arg Trp Arg Gln Arg Val Gln Val 465 470 475 480 Ala Phe Asp Ile Ala Asp Gly Leu Asn Tyr Leu His His Tyr Thr Asn 485 490 495 Pro Pro Cys Val His Lys Asn Leu Lys Ser Ser Asn Val Leu Leu Asp 500 505 510 Ala Asp Leu Arg Ala Lys Val Ser Gly Phe Gly Leu Ala Arg Ala Val 515 520 525 Thr Ala Ala His Gly Gly Ala Gln Leu Thr Gly His Val Val Gly Thr 530 535 540 Gln Gly Tyr Leu Ala Pro Glu Tyr Leu Glu Asp Gly Leu Ile Thr Pro 545 550 555 560 Lys Leu Asp Val Phe Ala Phe Gly Val Val Leu Leu Glu Leu Leu Ser 565 570 575 Gly Lys Glu Ala Gly Phe Ala Asp Ala Gly Thr Gly Glu Glu Ile Leu 580 585 590 Leu Cys Glu Ser Ala Glu Glu Ala Leu Val Ala Asp Gly Gly Glu Asp 595 600 605 Met Asp Arg Ala Lys Val Arg Ala Phe Met Asp Pro Arg Leu His Gly 610 615 620 Asp Phe Pro Met Asp Leu Ala Leu Ser Met Ala Ala Leu Ala Leu Arg 625 630 635 640 Cys Val Ala Met Glu Pro Arg Ala Arg Pro Ala Met Asp Glu Val Phe 645 650 655 Ile Ser Leu Ser Ala Val Tyr Asn Ser Thr Met Asp Cys Asp Pro Ser 660 665 670 Asp Tyr Gly Thr Ser Gly Ser Ser Met Ile Gly Arg 675 680 49 723 PRT 玉米 49 Met Asn Phe Ser Thr Trp Lys Thr Lys Glu Val Gln Val Arg Ser Phe 1 5 10 15 Thr Thr Arg Lys Pro Thr Pro Gln Ala Ala Cys Ala Met Ala Ser Phe 20 25 30 His Asp Leu Thr Ala Thr Ala Val Leu Leu Leu Leu Phe Ser Ile Leu 35 40 45 Ser Gly Gly Leu Ala Pro Leu Gln Val Gln Ala Gln Gln Pro Tyr Gly 50 55 60 Ser Gln Ile Ala Asp Cys Thr Asn Gln His Asn Ser Ser Ser Leu Leu 65 70 75 80 Gly Tyr Phe Cys Gly Ala Ala Gly Ser Ala Pro Ser Cys Pro Thr Phe 85 90 95 Leu Thr Phe Thr Ala Arg Ala Gln Tyr Ser Ser Leu Ala Thr Ile Gly 100 105 110 Ala Leu Leu Gly Ala Asp Pro Ala Ser Val Leu Ala Pro Asn Glu Ala 115 120 125 Thr Gly Ala Asp Ala Pro Leu Pro Ala Gly Thr Arg Val Leu Val Pro 130 135 140 Ala Thr Cys Ala Cys Thr Ala Thr Pro Gly Gly Arg Phe Tyr Gln Arg 145 150 155 160 Asn Ala Thr Tyr Val Ala Val Ala Gly Asp Thr Leu Leu Ile Ile Ala 165 170 175 Asn Asn Thr Phe Gln Gly Leu Thr Ser Cys Gln Ala Leu Glu Ala Gln 180 185 190 Ala Leu Arg Gly Ala Pro Pro Gln Ser Leu Asp Val Gly Gln Ser Leu 195 200 205 Pro Val Pro Leu Arg Cys Ala Cys Pro Ser Ala Ala Gln Ala Ala Ala 210 215 220 Gly Ala Arg Tyr Leu Val Ser Tyr Leu Val Asp Val Phe Asp Asp Leu 225 230 235 240 Thr Thr Val Ala Ala Arg Phe Gly Val Asp Met Gly Thr Val Ala Ala 245 250 255 Ser Asn Gln Leu Gln Pro Pro Phe Thr Ile Asp Pro Tyr Thr Thr Leu 260 265 270 Leu Ile Pro Val Ser Ala Gln Pro Asn Val Ser Arg Ile Gln Thr Pro 275 280 285 Pro Ser Pro Pro Pro Pro Pro Pro Val Val Ala Arg Ala Pro Ala Pro 290 295 300 Gly Lys Lys Ser Ser Asn His Val Gly Val Tyr Ile Gly Val Ala Val 305 310 315 320 Ala Val Val Val Val Ala Ala Ile Val Ser Ala Gly Ala Phe Leu Ala 325 330 335 Val Arg Ala Arg Arg Arg Arg Ala Gly Ala Val Leu Ala Thr Gly Glu 340 345 350 Val Ala Lys Lys Glu Ser Lys Ala Gly Asn Asp Arg Ala Ala Thr Ser 355 360 365 Ser Gly Phe Thr Gly Gly Glu Phe Ser Leu Ser Thr Ser Glu Ala Phe 370 375 380 Ser Ser Ile Ser Val Thr Asp Ile Lys Ser Ser Leu Lys Val Tyr Thr 385 390 395 400 Tyr Ala Glu Leu Lys Ala Ala Thr Asp Asp Phe Ser Pro Glu His Arg 405 410 415 Ile Gly Gly Ser Val Tyr Arg Ala Ala Phe Asn Gly Asp Ala Ala Ala 420 425 430 Val Glu Val Val Asp Arg Asn Val Ser Thr Glu Val Glu Ile Met Arg 435 440 445 Lys Ile Asn His Leu Asn Leu Ile Arg Leu Ile Gly Leu Cys His His 450 455 460 Arg Gly Arg Trp Tyr Leu Val Thr Glu Tyr Ala Glu His Gly Ala Leu 465 470 475 480 Arg Asp Arg Leu Leu Ala Ser Ala Thr Gly Thr Ala Ala Pro Leu Thr 485 490 495 Trp Ala Gln Arg Val His Ile Ala Leu Asp Val Ala Glu Gly Leu Arg 500 505 510 Tyr Leu His Glu Tyr Ala Arg Pro Ala Trp Val His Met Asp Val Ser 515 520 525 Ser Gly Ser Val Leu Leu Ala Gly Asp Gly Pro Arg Ala Lys Leu Arg 530 535 540 Gly Phe Gly Ala Ala Arg Ala Ile Thr Gly Ala Thr Ala Gly Val Asp 545 550 555 560 Gly Glu Glu Gly Ala Glu Glu Ala Leu Phe Thr Met Thr Ser Arg Ile 565 570 575 Ala Gly Thr Arg Gly Tyr Ile Ala Pro Glu Tyr Leu Glu His Gly Val 580 585 590 Val Ser Pro Lys Ala Asp Val Tyr Ser Leu Gly Val Val Leu Leu Glu 595 600 605 Leu Val Thr Gly Arg Asp Ala Glu Glu Leu Val Gly Asp Gly Val Gly 610 615 620 Asp Pro Phe Val Ala Leu Arg Glu Leu Ala Glu Glu Leu Asp Gly Gly 625 630 635 640 Gly Asp Ala Val Leu Gln Arg Leu Glu Glu Leu Val Asp Pro Ala Leu 645 650 655 Pro Ala Gly Ser Cys Pro Gln Asp Ala Val Val Met Val Val Arg Leu 660 665 670 Ile Glu Arg Cys Val Arg Gln Asp Pro Ala Arg Arg Pro Thr Thr Gly 675 680 685 Glu Val Ala Gln Arg Leu Leu Lys Leu Ser Gly Val Ser Val Val Ser 690 695 700 Trp Arg Asn Ser Pro Glu Ser Pro Arg Ser Ser Gly Ser Gly Lys Gly 705 710 715 720 Leu Met Tyr 50 587 PRT 玉米 50 Met Thr Lys His Gly Leu Leu Phe Phe Leu Ala Ile Ala Leu Leu Leu 1 5 10 15 His Arg His Tyr Thr Ser Ala Ser Ser Thr Gly Gln Pro Thr Arg Ser 20 25 30 Ser Ser Thr Ala Ser Arg Ser Thr Ala Glu Trp Gln Pro Leu His Cys 35 40 45 Ser Pro Val Ser Ser Cys Gly Ser Phe Leu Tyr Val Thr Pro Gly Gly 50 55 60 Arg Asn Leu Ser Glu Ile Ala Ser Val Phe Asn Gly Asn Ala Ser Leu 65 70 75 80 Ile Gln Pro Val Lys Arg Leu Ser Gly Ser Glu Asp Leu Leu Met Ala 85 90 95 Val Ala Cys Glu Cys Gln Ala Ile Ser Asn Thr Thr Thr Ala Ala Ala 100 105 110 Phe Leu His Asp Thr Gln Tyr Lys Val Glu Pro Asp Ala Ile Pro Asp 115 120 125 Asp Val Lys Ser Asn Thr Phe Ser Gly Leu Ala Met Asp Val Gly Asp 130 135 140 Gly Phe Pro Leu Thr Pro Gly Ala Thr Val Thr Val Arg Leu Pro Cys 145 150 155 160 Gly Cys Ser Ser Ser Thr Ala Ser Lys Gly Val Leu Ser Tyr Ser Val 165 170 175 Gln Glu Glu Asp Thr Leu Ser Thr Ile Ala Ser Leu Phe Ser Ser Ser 180 185 190 Pro Glu Ala Ile Leu Asn Leu Asn Pro Ser Val Lys Asn Pro Asp Phe 195 200 205 Ile Lys Pro Gly Trp Ile Leu Phe Val Pro Met Gly Val Ala Gly Ser 210 215 220 Ser Lys Lys Lys Arg Val Gly Ser Thr Thr Ile Thr Ile Ala Ala Ser 225 230 235 240 Val Ser Ala Ile Ile Leu Ser Val Cys Val Leu Thr Val Ile Leu Arg 245 250 255 Leu Arg Arg Arg Pro Ser Gln Gln Asn Ala Glu Ala Pro Glu Ile Lys 260 265 270 Met Glu Arg Ala Pro Asn Ile Asp Pro Phe Gln Thr Glu Arg Pro Val 275 280 285 Ile Phe Ser Leu Lys Val Val Gly Asp Ala Thr Ala Asn Phe Asp Glu 290 295 300 Lys Arg Lys Ile Gly Glu Gly Gly Tyr Gly Ser Val Tyr Leu Gly Phe 305 310 315 320 Ile Gly Thr His Glu Ile Ala Val Lys Lys Met Arg Ala Ser Lys Ser 325 330 335 Lys Glu Phe Phe Ala Glu Leu Lys Ala Leu Cys Lys Val His His Ile 340 345 350 Asn Val Val Glu Leu Ile Gly Tyr Ala Ala Gly Asp Asp His Leu Tyr 355 360 365 Leu Val Tyr Glu Tyr Val Gln Asn Gly Ser Leu Ser Glu His Leu His 370 375 380 Asp Pro Leu Leu Lys Gly His Gln Pro Leu Ser Trp Thr Ala Arg Thr 385 390 395 400 Gln Ile Ala Leu Asp Ala Ala Arg Gly Ile Glu Tyr Ile His Asp His 405 410 415 Thr Lys Ala Cys Tyr Val Ala Asp Phe Gly Leu Val Lys Leu Val Glu 420 425 430 Arg Ser Asp Glu Glu Glu Trp Val Ala Thr Arg Leu Val Gly Thr Pro 435 440 445 Gly Tyr Leu Pro Pro Glu Ser Val Leu Glu Leu His Met Thr Thr Lys 450 455 460 Ser Asp Val Tyr Ala Phe Gly Val Val Leu Ala Glu Leu Ile Thr Gly 465 470 475 480 Leu Arg Ala Leu Ile Arg Asp Asn Lys Glu Val Asn Lys Thr Lys Ser 485 490 495 Ile Ile Ser Ile Met Arg Lys Ala Phe Asp Ser Glu Asp Leu Glu Arg 500 505 510 Ser Leu Glu Thr Ile Ile Asp Pro Asn Leu Lys Asp Ser Tyr Pro Ile 515 520 525 Glu Glu Val Cys Lys Met Ala Asn Val Ser Met Trp Cys Leu Ser Glu 530 535 540 Asp Pro Leu Asn Arg Pro Glu Met Arg Asp Thr Met Pro Ala Leu Cys 545 550 555 560 Gln Ile His Leu Ala Ser Ile Glu Trp Glu Ala Ser Leu Gly Gly Asp 565 570 575 Gly Glu Val Phe Ser Gly Val Ser Tyr Gly Arg 580 585 51 629 PRT 玉米 51 Met Leu Tyr Pro His Ser Ala Gln Gln Thr Arg Thr Ala Trp Thr Thr 1 5 10 15 Arg Ser Arg Ser Arg Met Asp Thr Ala Cys His His Tyr Thr Ser Ala 20 25 30 Ser Phe Thr Asp Gln Pro Ser Ser Ile Ala Ser Ala Ala Glu Trp Gln 35 40 45 Pro Leu Thr Cys Asn Ala Ala Val Ser Asn Asn Pro Ser Cys Gly Ser 50 55 60 Phe Leu Tyr Val Thr Pro Arg Gly Arg Thr Leu Ser Glu Val Val Ser 65 70 75 80 Val Phe Asn Gly Asn Ala Ser Leu Ile Gln Pro Ile Lys Arg Leu Ser 85 90 95 Gly Ser Glu Asp Leu Leu Val Gly Val Ala Cys Lys Cys Glu Ala Ile 100 105 110 Asn Asp Thr Met Thr Ala Phe Phe His Asp Thr Gln Tyr Glu Val Glu 115 120 125 Pro Gly Asp Thr Pro Asp Asn Val Lys Ser Asn Asn Phe Ser Gly Leu 130 135 140 Ala Met Asn Val Gly Asp Gly Arg Thr Leu Ile Ala Gly Thr Thr Ile 145 150 155 160 Ala Val His Leu Pro Cys Gly Cys Ser Ser Thr Ala Pro Glu Gly Val 165 170 175 Leu Ser Tyr Ser Val Gln Glu Glu Asp Thr Leu Ser Thr Ile Ala Ser 180 185 190 Leu Phe Ser Ser Arg Gln Gln Asp Ile Leu Asn Leu Asn Pro Ile Leu 195 200 205 Arg Asn Ala Asp Phe Ile Arg Thr Gly Trp Ile Leu Phe Ile Pro Met 210 215 220 Gly Val Ala Gly Ser Ser Lys Lys Gly Ile Gly Ser Met Arg Ile Ile 225 230 235 240 Ile Ala Ala Ser Val Ser Ala Ala Val Leu Leu Phe Cys Val Leu Ala 245 250 255 Val Ile Leu Arg Arg Arg Arg Arg Ser Ser Gln His Asn Val Glu Ala 260 265 270 Pro Glu Ile Lys Met Glu Arg Ala Pro Ser Asn Thr Ser Ile Ala Ala 275 280 285 Leu Glu Ser Arg Phe Phe Pro Thr Met Arg Thr Asn Asp Thr Asp Pro 290 295 300 Phe Gln Thr Glu Arg Pro Val Ile Phe Ser Leu Lys Gln Val Gly Asp 305 310 315 320 Ala Thr Ala Asp Phe Ser Glu Lys Arg Lys Ile Gly Glu Gly Gly Tyr 325 330 335 Gly Ser Val Tyr Leu Gly Phe Ile Gly Ala His Glu Ile Ala Ile Lys 340 345 350 Lys Met Lys Ala Ser Lys Ser Lys Glu Phe Phe Ala Glu Leu Lys Ala 355 360 365 Leu Cys Lys Val His His Ile Asn Val Val Glu Leu Ile Gly Tyr Ala 370 375 380 Ala Gly Asp Asp His Leu Tyr Leu Val Tyr Glu Tyr Val Gln Asn Gly 385 390 395 400 Ser Leu Thr Asp His Leu His Asp Pro Leu Leu Lys Gly His Gln Pro 405 410 415 Leu Ser Trp Thr Ala Arg Thr Gln Ile Ala Leu Asp Ala Ala Arg Gly 420 425 430 Ile Glu Tyr Ile His Asp His Thr Lys Ala Cys Tyr Val His Arg Asp 435 440 445 Ile Lys Thr Ser Asn Ile Leu Leu Asp Asn Gly Leu Arg Ala Lys Val 450 455 460 Ala Asp Phe Gly Leu Val Lys Leu Val Glu Arg Ser Asp Glu Glu Glu 465 470 475 480 Phe Val Ala Thr Arg Leu Val Gly Thr Pro Gly Tyr Leu Pro Pro Glu 485 490 495 Ser Val Leu Glu Leu His Met Thr Thr Lys Ser Asp Val Tyr Ala Phe 500 505 510 Gly Val Val Leu Ala Glu Leu Ile Thr Gly Leu Arg Ala Leu Ile Arg 515 520 525 Asp Asn Lys Glu Val Asn Lys Thr Lys Ser Ile Thr Ser Ile Met Arg 530 535 540 Glu Val Phe Lys Ser Glu Asp Leu Glu Arg Ser Leu Glu Thr Ile Ile 545 550 555 560 Asp Pro Asn Leu Lys Asp Ser Tyr Pro Ile Glu Glu Val Cys Lys Met 565 570 575 Ala Asn Val Ser Met Trp Cys Leu Ser Glu Asp Pro Leu Asn Arg Pro 580 585 590 Glu Thr Arg Asp Ile Met Ser Thr Leu Gly Gln Ile His Leu Ala Ser 595 600 605 Ile Glu Trp Glu Ala Ser Leu Cys Gly Asp Gly Glu Val Phe Ser Gly 610 615 620 Val Ser Tyr Gly Arg 625 52 612 PRT 大麦 52 Met Ala Arg His Ser Leu Phe Phe Phe Phe Leu Ala Leu Ser Leu Gln 1 5 10 15 His Leu His Ile Ser Val Gly Leu Pro Gly Arg Ser Leu Ala Thr Ala 20 25 30 Thr Glu Gln Trp Gln Pro Met Gln Cys Asp Ala Ala Ser Leu Asn Ala 35 40 45 Ser Cys Ser Ser Tyr Leu Tyr Val Thr Pro Gln Gly Arg Ser Leu Ser 50 55 60 Glu Ile Ala Ser Leu Phe Asn Gly Ser Ala Ser Arg Thr Gln Pro Ile 65 70 75 80 Lys Arg Leu Ser Gly Ser Glu Asp Leu Leu Val Pro Val Pro Cys Met 85 90 95 Cys Asp Ala Ile Asn Asp Asn Met Ser Gly Leu Phe His Asp Thr Ala 100 105 110 Tyr Lys Val Asn Leu Asn Asp Thr Ala Asp Asn Ile Asn Ser Ile Phe 115 120 125 Ser Gly Leu Ala Trp Asn Ile Thr Ala Thr Ala Asn Thr Thr Ile Thr 130 135 140 Val His Leu Leu Cys Gly Cys Ser Ser Thr Ala Pro Glu Gly Val Ile 145 150 155 160 Ser Tyr Met Val Gln Ala Arg Asp Thr Leu Ser Asn Ile Ala Thr Leu 165 170 175 Phe Arg Ser Gly Ser Ser Glu Ile Leu Ser Leu Asn Ala Gly Val Thr 180 185 190 Asp Pro Asp Phe Leu Gln Pro Gly Trp Ile Leu Phe Ile Pro Met Gly 195 200 205 Val Ala Ser Ser Ser Lys Arg Lys Phe Gly Gly Leu Pro Ile Ile Ile 210 215 220 Ala Val Ser Ile Ser Ala Ala Ile Met Leu Leu Cys Thr Leu Thr Ile 225 230 235 240 Val Leu Arg Leu Arg Arg Arg Ser Leu Val Pro Asn Ala Glu Val Pro 245 250 255 Lys Lys Glu Met Glu Arg Val Pro Ser Asn Thr Ser Ile Ala Ile Leu 260 265 270 Glu Ser Arg Tyr Phe Pro Ser Lys Arg Ile Asp Asp Ile Asp Pro Phe 275 280 285 Gln Thr Glu Arg Pro Val Ile Phe Ser Leu Lys Ala Val Gly Glu Ala 290 295 300 Thr Ala Asn Phe Asp Glu Lys Arg Lys Ile Gly Glu Gly Gly Tyr Gly 305 310 315 320 Met Val Tyr Leu Gly Phe Ile Gly Thr His Glu Ile Ala Val Lys Met 325 330 335 Met Lys Asp Ser Lys Ser Lys Glu Phe Phe Ala Glu Leu Lys Val Leu 340 345 350 Cys Lys Val His His Ile Asn Val Val Glu Leu Ile Gly Tyr Ala Ser 355 360 365 Gly Glu Asp His Leu Tyr Leu Val Tyr Glu Tyr Val Gln Asn Gly Ser 370 375 380 Leu Ser Glu His Leu His Asp Pro Leu Leu Lys Gly His Gln Pro Leu 385 390 395 400 Ser Trp Thr Ala Arg Thr Gln Ile Ala Thr Asp Ala Ala Arg Gly Ile 405 410 415 Glu Tyr Ile His Asp His Thr Lys Ala Cys Tyr Val His Arg Asp Ile 420 425 430 Lys Thr Ser Asn Ile Leu Leu Asp Asp Gly Leu Arg Ala Lys Val Ala 435 440 445 Asp Phe Gly Leu Val Lys Leu Val Glu Arg Ser Asp Glu Glu Asp Cys 450 455 460 Leu Ala Thr Arg Leu Val Gly Thr Pro Gly Tyr Leu Pro Pro Glu Ser 465 470 475 480 Val Arg Glu Leu His Met Thr Thr Lys Ser Asp Val Tyr Ala Phe Gly 485 490 495 Val Val Leu Ala Glu Leu Ile Thr Gly Leu Arg Ala Leu Val Arg Asp 500 505 510 Asn Lys Glu Ala Asn Lys Thr Lys Ser Leu Ile Ser Thr Met Arg Lys 515 520 525 Ala Phe Lys Ser Glu Asp Val Glu Ser Ser Leu Glu Asn Ile Ile Asp 530 535 540 Pro Ser Leu Lys Asp Asn Tyr Pro Ile Glu Glu Val Cys Lys Leu Ala 545 550 555 560 Asn Ile Ser Met Trp Cys Leu Ser Glu Asp Pro Leu Asp Arg Pro Glu 565 570 575 Met Arg Glu Ile Met Pro Met Leu Ser Arg Ile His Leu Thr Ser Ile 580 585 590 Glu Trp Glu Ala Ser Leu Gly Gly Asp His Glu Val Phe Ser Gly Val 595 600 605 Phe Asn Gly Arg 610 53 673 PRT 玉米 53 Met Pro Pro His Arg Leu Leu Pro Leu Leu Leu Leu Leu Leu Pro Leu 1 5 10 15 Gly Val Ser Gly Ala Ala Ala Ala Gly Gly Asn Ala Thr Ser Ala Pro 20 25 30 Leu Pro Cys Ser Glu Leu Ser Arg Val Cys Thr Ala Phe Ile Ala Phe 35 40 45 Pro Thr Ala Gly Ala Gly Pro Ala Asn Ala Thr Val Leu Glu Ser Met 50 55 60 Phe Asp Ala Ala Pro Gly Asp Leu Thr Ala Asp Ala Ala Ala Ser Pro 65 70 75 80 Arg Tyr Ala Phe Val Arg Lys Asn Cys Ser Cys Leu Pro Ser Arg Thr 85 90 95 Tyr Leu Ala Asn Thr Thr Tyr Thr Ile Pro Ser Ser Ala Thr Thr Ser 100 105 110 Phe Pro Asn Thr Thr Ala Ala Asp Val Ala Ala Ala Ala Tyr Ser Gly 115 120 125 Leu Ala Val Pro Pro Pro Gly Gly Ala Ala Gln Arg Pro Pro Arg Pro 130 135 140 Gly Ala Val Val Ala Leu His Leu Leu Cys Gly Cys Ser Ser Gly Pro 145 150 155 160 Trp Asn Tyr Leu Leu Thr Tyr Val Gly Val Glu Gly Asp Thr Val Glu 165 170 175 Ser Leu Ser Ser Arg Phe Gly Ala Ser Met Asp Ala Ile Glu Thr Ala 180 185 190 Asn Ala Met Ala Gly Pro Asp Pro Ile Thr Ala Gly Lys Val Tyr Tyr 195 200 205 Ile Pro Leu Asn Ser Val Pro Gly Gln Ala Tyr Val Thr Leu Pro Ala 210 215 220 Pro Pro Ala Pro Ala Pro Ala Pro Thr Asp Tyr Thr Leu Ser Gly Thr 225 230 235 240 Pro Asp Tyr His Ser Ser Lys Phe Pro Tyr Gly Trp Val Ile Gly Ser 245 250 255 Met Gly Val Ala Leu Ala Leu Ile Val Ile Ala Val Leu Ala Leu Val 260 265 270 Leu Trp Lys Phe Phe Gly Tyr Lys Pro Gln Asp Arg Asn Gly Gln Arg 275 280 285 Lys Ser Pro Asp Arg His Lys Phe Gln Leu Leu Lys Ser Gly Ser Phe 290 295 300 Cys Tyr Gly Ser Gly Arg Tyr Leu Cys Cys Gln Phe Gly Asn Ala Lys 305 310 315 320 Pro Thr Arg Ala Asp Gly Gly Glu His His Ile Asn Val Pro Lys Gly 325 330 335 Val Ala Ala Asp Val Phe Asp Arg Glu Lys Pro Ile Val Phe Thr His 340 345 350 Glu Glu Ile Leu Ile Ser Thr Asp Ser Phe Ser Asp Ala Asn Leu Leu 355 360 365 Gly His Gly Thr Tyr Gly Ser Val Tyr Tyr Gly Val Leu Arg Glu Gln 370 375 380 Glu Val Ala Ile Lys Arg Met Met Ala Thr Lys Thr Lys Glu Phe Ile 385 390 395 400 Val Glu Met Lys Val Leu Cys Lys Val His His Ala Ser Leu Val Glu 405 410 415 Leu Ile Gly Tyr Ala Ala Gly Lys Asp Glu Leu Phe Leu Val Tyr Glu 420 425 430 Tyr Ser Gln Asn Gly Ser Leu Lys Asn His Leu His Asp Pro Glu Arg 435 440 445 Lys Gly Cys Ser Ser Leu Ser Trp Ile Phe Arg Val Gln Ile Ala Leu 450 455 460 Asp Ala Ala Arg Gly Leu Glu Tyr Ile His Glu His Thr Lys Asp His 465 470 475 480 Tyr Val His Arg Asp Ile Lys Ser Ser Asn Ile Leu Leu Asp Gly Ser 485 490 495 Phe Arg Ala Lys Ile Ser Asp Phe Gly Leu Ala Lys Leu Val Val Lys 500 505 510 Ser Asn Asp Ala Glu Ala Ser Val Thr Lys Val Val Gly Thr Phe Gly 515 520 525 Tyr Leu Ala Pro Glu Tyr Leu Arg Asp Gly Leu Ala Thr Thr Lys Ser 530 535 540 Asp Val Tyr Ala Phe Gly Val Val Leu Phe Glu Leu Ile Ser Gly Lys 545 550 555 560 Glu Ala Ile Thr Arg Ala Glu Gly Met Gly Ala Ser Ser Asn Ser Glu 565 570 575 Arg Cys Ser Leu Ala Ser Val Met Leu Ala Ala Val Arg Lys Cys Pro 580 585 590 Asn Ser Thr Tyr Met Gly Asn Leu Lys Asp Cys Ile Asp His Asn Leu 595 600 605 Arg Asp Leu Tyr Pro Tyr Asp Cys Ala Tyr Lys Met Ala Met Leu Ala 610 615 620 Lys Gln Cys Val Asp Glu Asp Pro Val Leu Arg Pro Asp Met Lys Gln 625 630 635 640 Val Val Ile Thr Leu Ser Gln Ile Leu Leu Ser Ser Ile Glu Trp Glu 645 650 655 Ala Thr Gln Ala Gly Asn Ser Gln Val Phe Ser Gly Leu Val Ala Gly 660 665 670 Arg 54 722 PRT 大麦 54 Met Pro Pro Arg Arg Arg Leu Leu Leu Leu Leu Leu Ala Leu Ala Cys 1 5 10 15 Ser Gly Gly Gly Ala Ala Val Asp Thr Ala Pro Gly Asn Gly Thr Ser 20 25 30 Ser Pro Leu Ala Cys Ser Glu Leu Ser Arg Val Cys Thr Ala Phe Leu 35 40 45 Ala Phe Pro Ala Ala Gly Asn Ala Ser Val Leu Gln Ser Met Phe Asp 50 55 60 Ala Ser Pro Gly Asp Leu Thr Ser Asp Pro Ala Ala Ser Pro Gly Tyr 65 70 75 80 Ala Phe Val Arg Lys Asn Cys Ser Cys Leu Ala Ser Arg Thr Tyr Leu 85 90 95 Ala Asn Thr Thr Tyr Thr Ile Pro Ser Thr Val Pro Leu Asn Ala Thr 100 105 110 Ala Ala Gln Val Ala Ala Ala Ala Tyr Gly Gly Leu Ala Val Pro Pro 115 120 125 Pro Gly Gly Ala Leu Gln Arg Pro Pro Arg Pro Gly Ala Val Val Ala 130 135 140 Leu His Leu Ile Cys Gly Cys Ser Ser Gly Pro Trp Asn Tyr Leu Leu 145 150 155 160 Ser Tyr Val Gly Ser Asp Gly Asp Thr Val Glu Ser Leu Ser Ser Arg 165 170 175 Phe Gly Ala Ser Met Asp Ala Ile Glu Ala Ala Asn Gly Met Pro Gly 180 185 190 Pro Asp Pro Ile Thr Thr Gly Lys Val Tyr Tyr Ile Pro Leu Asn Ser 195 200 205 Val Pro Gly Gln Pro Tyr Val Ala Met Ser Ser Ala Pro Val Pro Ala 210 215 220 Pro Ala Pro Thr Gln Asn Thr Leu Ser Glu Ile Ser Asp His His Ser 225 230 235 240 Ala Lys Phe Pro Tyr Gly Trp Val Ile Gly Gly Met Gly Val Ala Leu 245 250 255 Ala Leu Ile Ala Ile Ala Leu Leu Ala Leu Leu Met Cys Lys Ser Phe 260 265 270 Gln Tyr Asn His Gln Gly Ser Asn Asn Gln Gly Lys Ser Pro Asp Gln 275 280 285 Pro Met Pro His Asn Phe Gln Leu Leu Lys Ser Gly Ser Phe Cys Tyr 290 295 300 Gly Ser Gly Arg Tyr Phe Cys Cys Gln Phe Gly Asn Ala Lys Gln Ser 305 310 315 320 Arg Lys Gly Gly Glu Asp His His Ile Asn Val Pro Lys Gly Met Val 325 330 335 Val Asp Val Phe Asp Arg Glu Lys Pro Ile Val Phe Thr Tyr Glu Glu 340 345 350 Ile Leu Ala Ser Thr Asp Leu Phe Ser Asp Ala Asn Leu Leu Gly His 355 360 365 Gly Thr Tyr Gly Ser Val Tyr Tyr Gly Val Leu Arg Asp Gln Glu Val 370 375 380 Ala Ile Lys Arg Met Thr Ser Thr Asn Thr Lys Glu Phe Ile Val Glu 385 390 395 400 Met Lys Val Leu Cys Lys Val His His Ala Ser Leu Val Glu Leu Ile 405 410 415 Gly Tyr Ala Ala Ser Lys Asp Glu Leu Phe Leu Val Tyr Glu Tyr Ser 420 425 430 Gln Lys Gly Ser Leu Arg Asn His Leu His Asp Pro Gln Ser Lys Gly 435 440 445 Tyr Thr Ser Leu Ser Trp Ile Tyr Arg Val Gln Ile Ala Leu Asp Ala 450 455 460 Ala Arg Gly Leu Glu Tyr Ile His Glu His Thr Lys Asp His Tyr Val 465 470 475 480 His Arg Asp Ile Lys Ser Ser Asn Ile Leu Leu Asp Gly Ser Phe Arg 485 490 495 Ala Lys Ile Ser Asp Phe Gly Leu Ala Lys Leu Gly Leu Arg Ser Asn 500 505 510 Asp Ala Glu Ala Ser Val Thr Lys Val Val Gly Thr Phe Gly Tyr Leu 515 520 525 Ala Pro Glu Tyr Leu Arg Asp Gly Leu Ala Thr Ala Lys Cys Asp Val 530 535 540 Tyr Ala Phe Gly Val Val Leu Phe Glu Leu Ile Ser Gly Lys Glu Ala 545 550 555 560 Ile Thr Lys Ala Asp Ala Val Gly Ala Ser Ser Asn Ser Glu Arg Arg 565 570 575 Ser Leu Ala Ser Val Val Ser Phe Leu Thr Cys Thr Gln Ala Val Ile 580 585 590 Gln Ser Thr Ala Cys Val Phe Ala Val Ile Ser Leu Pro Lys Val Tyr 595 600 605 Ile Gly Ile Ser Ser Thr Ser Phe Tyr Thr Ser Asn Leu Lys Asp Leu 610 615 620 Ser Arg Phe Gly Leu Thr Gly Gln Met Leu Thr Ala Leu Arg Asn Cys 625 630 635 640 His Asp Pro Thr Cys Val Gly Ser Leu Lys Asp Cys Ile Asp Pro Asn 645 650 655 Leu Met Asp Leu Tyr Pro His Asp Cys Ile Tyr Gln Met Ala Met Leu 660 665 670 Ala Lys Gln Cys Ala Asp Glu Asp Pro Val Leu Arg Pro Asp Met Lys 675 680 685 Gln Ala Val Ile Thr Leu Ser Gln Ile Leu Leu Ser Ser Ile Glu Trp 690 695 700 Glu Ala Thr Leu Gly Gly Asn Ser Gln Val Phe Ser Gly Leu Val Ala 705 710 715 720 Gly Arg 55 613 PRT 大麦 55 Met Pro Pro Pro Pro Ala Tyr His His Leu Leu Leu Leu Leu Leu Leu 1 5 10 15 Leu Leu Arg Leu His Gly Ala Ile Ala Ser Ala Thr Gly Phe Thr Cys 20 25 30 Thr Lys Pro Ser Thr Cys Gln Ser Ala Val Ile Gly Tyr Val Val Pro 35 40 45 Asn Thr Ile Thr Tyr Lys Glu Leu Ile Ser Gln Phe Ser Pro Thr Thr 50 55 60 Leu His Asp Val Val Ala Ala Asn Gln Leu Pro Phe Asn Thr Ala Thr 65 70 75 80 Lys Gln Val Ile Pro Pro Lys Thr Thr Leu Thr Ile Pro Phe Arg Cys 85 90 95 Arg Cys Thr Gly Asn Gly Ile Gly Gln Ser Gly Leu Tyr Ile Ala Gln 100 105 110 Asn Lys Leu Asp Asp Gly Leu Ala Thr Tyr Gln Pro Glu Ile Val Ser 115 120 125 Ser Lys Ser Ile Ala Asp Asn Ala Ala Asn Trp Lys Gly Trp Ile Pro 130 135 140 Leu Pro Cys Ser Cys Asp Gly Ala Asp Val Thr His Phe Pro Tyr Ile 145 150 155 160 Val Arg Ser Gly Asp Ser Ala Leu Ala Ile Ala Ala Lys Tyr Gly Val 165 170 175 Leu Leu Ser Val Leu Leu Glu Ile Asn Asn Ile Thr Asn His Ala Ser 180 185 190 Leu Tyr Gln Gly Gln Val Leu Asp Ile Pro Leu Gln Gly Lys Val Gly 195 200 205 Glu Glu Leu Ser Ser Met Gly Arg Trp Ser Arg Val Tyr Tyr Ile Ser 210 215 220 Gly Tyr Arg Lys Arg Arg Leu Gly Trp Phe Asn Ser Ala Ala Ala Glu 225 230 235 240 Gln Ser Ala His Ala Ala Ala Glu Ala Val Ala Ala Thr Lys Glu Ala 245 250 255 Ala Glu Asn Ser His Tyr Ser Pro Glu Ala Ala Asp Thr Val Val Lys 260 265 270 Arg Val Leu Val Leu Leu Leu Ile Ile Ile Ile Leu Ser Leu Leu Tyr 275 280 285 Phe Thr Phe Tyr Tyr Trp Lys Ser Ala Cys Glu Ser Leu Ser Ser Arg 290 295 300 Thr Asn Gly Val Ile Gln Phe Tyr Tyr Ser Asp Leu Ala Arg Ala Thr 305 310 315 320 His Arg Phe Ser Lys Glu Ser Lys Ile Gly Glu Gly Gln Tyr Gly Thr 325 330 335 Val Tyr Lys Ala Thr Ile Lys Gly His Glu Met Ala Val Lys Lys Leu 340 345 350 Lys Ala Glu Gly Glu Thr Lys Glu Leu His Arg Glu Leu Gln Thr Ile 355 360 365 Ser Asn Thr Lys His Thr Asn Leu Val Ser Leu Lys Gly Trp Cys Gly 370 375 380 Arg Leu Arg Leu Ile Asp Gly Lys Ser Cys Trp Lys Arg Gln Ile Lys 385 390 395 400 Val Glu Leu Leu Leu Val Phe Glu Trp Ile Pro Asn Gly Asn Leu Ala 405 410 415 Asp His Leu His Asn Arg Glu Gln Val Leu Ser Trp Glu Lys Arg Tyr 420 425 430 Lys Ile Val Lys Gly Ile Gly Ser Ala Leu Arg Tyr Leu His His Glu 435 440 445 Cys Lys Pro Ser Ile Leu His Arg Asp Ile Lys Pro Asp Asn Ile Leu 450 455 460 Leu Asp Tyr His Phe Asn Ala Lys Leu Ala Asp Phe Gly Leu Ser Met 465 470 475 480 Ile Thr Asp Gln Asn Gly Ala Thr Val Phe Thr Ile Ala Ile Gly Pro 485 490 495 Arg Arg Tyr Met Asp Pro Gln Leu Met Lys Glu Gly Glu Phe Arg Phe 500 505 510 Asn His Lys Ser Asp Ile Tyr Ser Phe Gly Ile Val Leu Leu Glu Ile 515 520 525 Ala Cys Thr Gly Lys Ser Arg Glu Asn Ile Leu His Ile Leu Gly Gly 530 535 540 Gly Ser Gly Gln His Val Gln Val Asp Gly Leu Ala Asp His Arg Leu 545 550 555 560 Ser Ile Phe Asp Arg Thr Glu Met Ala Arg Val Val Val Leu Gly Leu 565 570 575 Gln Cys Ser His Pro Asp Glu Arg Gln Arg Pro Ser Met Tyr Met Ala 580 585 590 Met Arg Phe Leu Glu Glu Gly Ile Glu Leu Pro Ile Ala Ser His Asn 595 600 605 Arg Arg Glu Arg Leu 610 56 416 PRT 拟南芥 56 Met Lys Ile Pro Glu Lys Pro Ile Phe Leu Ile Phe Val Ser Leu Ile 1 5 10 15 Leu Ala Ser Ser Leu Thr Phe Thr Ala Thr Ala Lys Ser Thr Ile Glu 20 25 30 Pro Cys Ser Ser Asn Asp Thr Cys Asn Ala Leu Leu Gly Tyr Thr Leu 35 40 45 Tyr Thr Asp Leu Lys Val Ser Glu Val Ala Ser Leu Phe Gln Val Asp 50 55 60 Pro Ile Ser Ile Leu Leu Ala Asn Ala Ile Asp Ile Ser Tyr Pro Asp 65 70 75 80 Val Glu Asn His Ile Leu Pro Ser Lys Leu Phe Leu Lys Ile Pro Ile 85 90 95 Thr Cys Ser Cys Val Asp Gly Ile Arg Lys Ser Val Ser Thr His Tyr 100 105 110 Lys Thr Arg Pro Ser Asp Asn Leu Gly Ser Ile Ala Asp Ser Val Tyr 115 120 125 Gly Gly Leu Val Ser Ala Glu Gln Ile Gln Glu Ala Asn Ser Val Asn 130 135 140 Asp Pro Ser Leu Leu Asp Val Gly Thr Ser Leu Val Ile Pro Leu Pro 145 150 155 160 Cys Ala Cys Phe Asn Gly Thr Asp Asn Ser Leu Pro Ala Val Tyr Leu 165 170 175 Ser Tyr Val Val Lys Glu Ile Asp Thr Leu Val Gly Ile Ala Arg Arg 180 185 190 Tyr Ser Thr Thr Ile Thr Asp Leu Met Asn Val Asn Ala Met Gly Ala 195 200 205 Pro Asp Val Ser Ser Gly Asp Ile Leu Ala Val Pro Leu Ser Ala Cys 210 215 220 Ala Ser Lys Phe Pro Arg Tyr Ala Ser Asp Phe Gly Leu Ile Val Pro 225 230 235 240 Asn Gly Ser Tyr Ala Leu Ala Ala Gly His Cys Val Gln Cys Ser Cys 245 250 255 Ala Leu Gly Ser Arg Asn Leu Tyr Cys Glu Pro Ala Ser Leu Ala Val 260 265 270 Ser Cys Ser Ser Met Gln Cys Arg Asn Ser Asn Leu Met Leu Gly Asn 275 280 285 Ile Thr Val Gln Gln Thr Ser Ala Gly Cys Asn Val Thr Thr Cys Asp 290 295 300 Tyr Asn Gly Ile Ala Asn Gly Thr Ile Leu Thr Met Leu Thr Arg Ser 305 310 315 320 Leu Gln Pro Arg Cys Pro Gly Pro Gln Gln Phe Ala Pro Leu Leu Ala 325 330 335 Pro Pro Asp Thr Val Pro Arg Asp Val Met Tyr Ala Pro Ala Pro Ser 340 345 350 Pro Asp Phe Asp Gly Pro Gly Ser Ile Ala Ser Ser Pro Arg Ser Ser 355 360 365 Met Leu Pro Gly Gly Gly Ile Leu Pro Gly Asn Pro Ala Asn Gly Pro 370 375 380 Ala Gly Ser Ile Ser Thr Ala Ser Ala Ser Ser Val Ser Tyr Phe Phe 385 390 395 400 Ile Thr Phe Leu Ile Ser Ile Ala Ser Phe Ser Leu Ala Leu Ser Ser 405 410 415 57 415 PRT 百脉根 57 Met Arg Met Gln Gln Gln Gln Ser Thr Leu Leu Phe Leu Val Val Leu 1 5 10 15 Leu Phe His Ser Leu Thr Thr Thr Thr Cys Lys Ser Thr Ile Glu Pro 20 25 30 Cys Thr Asn Ser Asp Ser Cys Asn Ala Leu Leu Gly Tyr Thr Leu Tyr 35 40 45 Thr Asp Leu Lys Val Ser Glu Val Ala Ser Leu Phe Gly Ile Asp Pro 50 55 60 Ile Ser Leu Leu Thr Ala Asn Ala Ile Asp Ile Ser Tyr Pro Asp Ala 65 70 75 80 Glu His His Ile Leu Pro Pro Lys Leu Phe Leu Lys Ile Pro Ile Ser 85 90 95 Cys Ser Cys Val Asp Gly Ile Arg Lys Ser Val Ser Thr Ser Tyr Lys 100 105 110 Thr Arg Pro Ser Asp Thr Leu Ser Ser Ile Ala Asp Ser Val Tyr Gly 115 120 125 Gly Leu Val Ser Ala Asp Gln Leu Thr Asp Pro Ser Val Leu Asp Val 130 135 140 Gly Gln Ser Leu Val Val Pro Leu Pro Cys Thr Cys Phe Asn Gly Ser 145 150 155 160 Asp Asn Ser Leu Pro Ala Ile Tyr Leu Ser Tyr Val Val Gln Pro Val 165 170 175 Asp Ser Leu Ala Ala Ile Ala Ala Arg Tyr Leu Thr Thr Leu Thr Asp 180 185 190 Leu Met Asn Val Asn Ala Met Gly Ser Thr Ala Ile Ser Asp Gly Asp 195 200 205 Ile Leu Ala Val Pro Ile Pro Ala Cys Ala Ser Asn Phe Pro Lys Ser 210 215 220 Ala Ser Asp Phe Gly Leu Leu Val Pro Asn Gly Ser Tyr Ala Ile Thr 225 230 235 240 Ala Gly His Cys Val Gln Cys Ser Cys Gly Pro Arg Asn Leu Asn Leu 245 250 255 Tyr Cys Met Pro Thr Ser Leu Ser Ala Ser Cys Ser Ser Met Gln Cys 260 265 270 Lys Asn Ser Asn Leu Met Leu Gly Asn Val Thr Ala Gln Gln Ser Ser 275 280 285 Ala Gly Cys Asn Val Ser Ser Cys Ser Tyr Asp Gly Leu Val Asn Gly 290 295 300 Thr Ile Ala Thr Thr Leu Ser Ala Ser Leu Gln Pro Arg Cys Pro Gly 305 310 315 320 Leu Gln Glu Phe Pro Pro Leu Val Ala Pro Pro Thr Ser Val Glu Lys 325 330 335 Asp Pro Thr Phe Ala Ser Gly Pro Ala Pro Ser Pro Ser Pro Gln Ser 340 345 350 His Gly Ser Gly Leu Pro Ser Pro Lys Ser Ser Gly Met Pro Gly Leu 355 360 365 Pro Gly Phe Ser Pro Ala Asn Gly Pro Val Ser Gly Ile Ser Ser Gly 370 375 380 Ala Ser Ala Ala Cys Ser Leu Val Lys Pro Ser Pro Thr Leu Thr Ser 385 390 395 400 Ala Leu Val Leu Leu Leu Ala Met Leu Val Ile Pro Val Ala Leu 405 410 415 58 394 PRT 百脉根 58 Met Arg Asn His Leu Gln Phe Leu Trp Arg His Ile Leu Val Phe Leu 1 5 10 15 Leu Leu Ser Val Ser Tyr Gln Val Glu Ala Lys Ser Thr Ile Glu Pro 20 25 30 Cys Ser Ser Gly Phe Pro Cys Pro Ser Leu Leu Ser Tyr Ile Leu Pro 35 40 45 Trp Asp Ser Lys Leu Ser Glu Ile Ala Thr Arg Phe Ser Val Asn Val 50 55 60 Ser Asn Ile Leu Ala Ala Asn Ser Val Phe Pro Ile Thr Pro Ser Ser 65 70 75 80 Gly His Gln Ile Leu Ser Ala Lys Ser Ile Val Lys Ile Pro Phe Ser 85 90 95 Cys Pro Cys Val Asp Gly Ile Arg Arg Ser Ile Ser Thr Ile Tyr Asn 100 105 110 Val Glu Ala Ser Asp Thr Leu Ala Ser Ile Ser Glu Gly Tyr Gly Gly 115 120 125 Leu Val Ser Ala Glu Gln Ile Lys Thr Met Asn Ser Ile Asn Glu Thr 130 135 140 Asn Pro Leu Thr Tyr Gly Ser Ser Ile Val Ile Pro Leu Pro Cys Lys 145 150 155 160 Cys Leu Asn Asn Val Asn Asn Gly Asp Thr Thr Val Tyr Met Ser Tyr 165 170 175 Val Val Gln Lys Gly Gln Ser Leu Gly Ser Ile Ala Thr Met Tyr Gly 180 185 190 Thr Thr Val Ser Asp Leu Glu Ser Val Asn Gly Leu Gly Gln Asn Ala 195 200 205 Val Asp Pro Gly Asp Ile Leu Ser Val Pro Val Ala Ala Cys Ser Ser 210 215 220 Ala Thr Leu Asn Trp Tyr Ser Glu Asn Leu Ile Val Pro Asn Gly Ser 225 230 235 240 Tyr Ile Leu Thr Ala Ser Asn Cys Ile Gln Cys Thr Cys Thr Pro Arg 245 250 255 Asp Leu Lys Met Glu Cys Leu Pro Ser Gly Met Asp Val Pro Cys Tyr 260 265 270 Asn Leu His Cys Lys Gly Ser Asn Leu Ile Ile Gly Asn Glu Tyr Val 275 280 285 Glu His Ser Gln Thr Ser Cys Asn Val Ser Gln Cys Val Tyr Arg Gly 290 295 300 His Arg Gly Gly Lys Ile Leu Ser Ser Ile Ile Asn Ser Ser Tyr Leu 305 310 315 320 Gln Cys Pro Asp Asn Gln Ser Tyr Ser Gly Pro Ser Arg Trp Pro Ser 325 330 335 Leu Thr Pro Tyr Ala Ala Glu Tyr Pro Phe Asp Ile Ser Pro Ser Pro 340 345 350 Ser Ser Pro Pro Leu Pro Val Ser Glu Ala Ala Leu Arg Thr Arg Ala 355 360 365 Ser Gly Gly Trp Gln Gly Gln Ser Leu Ile Asn Val Met Gln Leu Phe 370 375 380 Leu Ile Lys Leu Ile Leu Tyr Phe Ile Met 385 390 59 370 PRT 百脉根 59 Met Gly Thr Val Trp Leu Ser Lys Leu Val Ala Thr Thr Met Leu Val 1 5 10 15 Ala Val Leu Gly Leu Leu Ala Glu Ala Gln Ile Glu Ala Lys Phe Lys 20 25 30 Cys Ile Ser Glu Asn Ala Pro Cys His Ala Leu Ala Asp Tyr Ser His 35 40 45 Pro Asn Gly Thr Thr Leu Arg Arg Ile Gln Thr Leu Phe Thr Val Lys 50 55 60 Tyr Leu Pro Asp Ile Leu Gly Ala Asn Asn Leu Pro Ala Asn Thr Thr 65 70 75 80 Arg Val Ala Pro Asp Gln Val Ile Lys Val Pro Phe Pro Cys Arg Cys 85 90 95 Ser Asn Gly Thr Gly Leu Ser Asn Lys Val Pro Arg Tyr Lys Ile Lys 100 105 110 Lys Gly Asp Thr Leu Tyr Asp Ile Ala Thr Thr Val Phe Ala Gly Leu 115 120 125 Val Lys Tyr Pro Gln Ile Gln Val Ala Asn Glu Ile Pro Asp Ala Asn 130 135 140 Asn Ile Thr Ala Gly Asp Thr Ile Trp Ile Pro Leu Pro Cys Ser Cys 145 150 155 160 Asp Ala Val Ala Gly Ser Ser Val Val His Tyr Ala His Leu Val Gln 165 170 175 Asp Gly Ser Ser Val Glu Ser Ile Ala Gln Glu Tyr Gly Ser Thr Gln 180 185 190 Gln Ile Leu Leu Ser Leu Asn Gly Ile Ser Asp Pro Lys Leu Leu Gln 195 200 205 Ala Arg Gln Leu Leu Asp Val Pro Leu Gln Ala Cys Ser Ser Ser Val 210 215 220 Lys Asn Asp Ser Pro Asp Tyr Pro Leu Leu Val Pro Asn Ala Thr Tyr 225 230 235 240 Val Tyr Thr Ala Lys Glu Cys Val Lys Cys Lys Cys Asp Ser Ser Asn 245 250 255 Asn Phe Arg Leu Gln Cys Glu Pro Ser Gln His Lys Pro Ile Asn Asp 260 265 270 Trp Ser Val Cys Pro Ser Met Glu Cys Ser Lys Asn Val Leu Ile Gly 275 280 285 Asn Thr Thr Ser Thr Asp Ser Cys Asn Arg Thr Ile Cys Asp Tyr Ala 290 295 300 Gly Tyr Ser Asn Ser Lys Ile Ser Thr Ile Leu Ala Thr Gln Asn Thr 305 310 315 320 Cys Ala Val Pro Pro Ser Gly Ser Gly Thr Ser Ser Gly Ser Gly Ser 325 330 335 Gly Asp Ser Gly Ser Gly Ala Ser Arg Ser Asn Leu His Gly Trp Val 340 345 350 Trp Ser Ser Pro Leu Ile Val Ile His Phe Leu Leu Phe Val Val Phe 355 360 365 Leu Leu 370 |
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